Method of cultivating detoxicated ginger seedling

A cultivation method and a technology of virus-free seedlings are applied in the field of production of virus-free ginger seedlings to achieve a good effect of popularization and application

Inactive Publication Date: 2007-03-21
徐坤 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about the method of ginger virus-free seedling cultivation at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Obtaining the regenerated plant of ginger isolated shoot tip culture: wash the 'Laiwu Dajiang' ginger piece with clean water, then soak it in 500 times 50% carbendazim solution for 30 minutes, and bury it in the wet sand washed with clean water. After germination in a room with a temperature of 22-28°C for 25 days, ginger buds with a length of about 1.2 cm were taken, sterilized with 70% ethanol for 30 minutes, then sterilized with 0.1% mercury liter for 4 minutes, rinsed with sterile water for 5 times, and then placed in Under the three-dimensional dissecting microscope, peel off the ginger buds until only 1 or 2 leaf primordia remain, and inoculate a single bud on the MS medium supplemented with KT 2.0mg / L, NAA 0.25mg / L, sucrose 25g / L, and pH 6.5. 100 bottles. The culture conditions are temperature 26±2°C, light 12h / d, light intensity 4000lx. After culturing for 35 days, 86 bottles of in vitro regenerated plants with a plant height of 5-6 cm were obtained, and the...

Embodiment 2

[0029]1. Primary culture of ginger isolated shoot apex: take about 1cm sprouts from ginger rhizomes, sterilize them with 0.1% mercuric chloride, peel them off under a stereoscopic dissecting microscope until only 1-2 leaf primordia remain, and inoculate them with additional KT 2.5mg / L, NAA 0.30mg / L, sucrose 25g / L, pH 6.5 MS medium, the culture conditions are temperature 20~28℃, light 12h / d, light intensity 2000lx, culture for 35d, the plant height 5~ 7cm test-tube seedlings of regenerated plants in vitro.

[0030] 3. Ginger test-tube plantlet virus detection: take the test-tube plantlet of the regenerated plant of ginger isolated shoot tip, cut off the stem and leaf, put it in a sterile mortar, add an appropriate amount of buffer solution (PBS 0.05M, pH 7.0, containing 0.1% mercaptoethanol ) ground, inoculated on Nicotiana qilutinosa cultivated in sterilized soil by conventional methods, placed in an insect-proof net room or greenhouse for cultivation, observed and recorded a...

Embodiment 3

[0034] 1. Acquisition of virus-free ginger test-tube seedlings: Take the sprouts germinated from the rhizomes of general ginger, peel off until only 1-2 leaf primordia remain, and inoculate them with additional KT 2.0mg / L, NAA 0.30mg / L, sucrose 25g / L, On MS medium with pH 6.5, the culture conditions are temperature 22-28°C, light 12h / d, and light intensity 4000lx. Cultivate for 35 days to obtain test-tube plantlets of regenerated plants from isolated shoot tips of ginger, cut the stems and leaves of test-tube plantlets for virus detection, divide the base of non-virus-infected cluster test-tube plantlets into single buds, and re-transfer to new culture bottles for subculture , to obtain detoxified ginger test-tube plantlets;

[0035] 2. Rapid propagation of virus-free ginger test-tube seedlings: cut the stems and leaves of the virus-free ginger test-tube seedlings, divide the base of the test-tube seedlings into single buds, and re-inoculate them with new additional KT 2.5mg / L...

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Abstract

The present invention belongs to the field of method of cultivating detoxicated ginger seedling in agricultural technology. The present invention features that the process of cultivating detoxicated ginger seedling includes the following steps: inoculating young ginger bud after being stripped to 1-2 leaf primordiums to MS culture medium with KT in 2.0-2.5mg / L, NAA in 0.25-0.30mg / L, cane sugar in 25g / L and pH 6.5 to culture for 30-35 days so as to obtain regenerated test tube plantlet; cutting stem and leaf for viral detection and re-inoculating the no-virus test tube plantlet to MS culture medium with KT in 2.5-3.0mg / L and NAA in 0.30-0.40mg / L to culture for other 40-50 days so as to obtain detoxicated test tube plantlet; and transferring the detoxicated test tube plantlet to rooting MS culture medium with NAA 0.50-1.0mg / L to culture for other 40-50 days so as to obtain detoxicated ginger seedling with complete roots, stem and leaves.

Description

1. Technical field [0001] The technical invention relates to a production technical method related to the virus-free ginger seedlings, which is specially used for the cultivation and rapid propagation of the virus-free ginger seedlings, and belongs to the field of agricultural high-tech. 2. Background technology [0002] Ginger (Zingiber officiale Roscoe) is one of the main foreign exchange-earning vegetables for export in my country. It is a vegetatively propagated crop with rhizomes as product organs and reproductive organs. Because its growth and development have not had sexual generations, viruses are easy to accumulate in the body, resulting in growth vigor. Recession, species degradation, reduced stress resistance, aggravated pests and diseases, and reduced yield and quality have seriously restricted the competitiveness of ginger products in the international market. Therefore, cultivating virus-free ginger seedlings and recovering ginger seedlings is one of the importa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00
Inventor 徐坤郑永强
Owner 徐坤
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