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Scale tissue culture quickly reproducing method of saffron crocus seedball (sprout)

A technology of tissue culture rapid propagation and saffron, which is applied in the field of biochemical engineering, can solve problems such as the shortage of saffron resources, and achieve the effect of solving the shortage of resources

Inactive Publication Date: 2007-05-02
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adopting the saffron corm bred by the present invention can not only reduce or eliminate disease infection, but also can solve the long-term contradiction of relying on imported bulbs and flower production and bulb reproduction, lay the foundation for large-scale planting of saffron, and fundamentally solve the shortage of saffron resources. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Add 2,4-dichlorophenoxyacetic acid 2mg / L, N 6 - Benzyl adenine 0.25mg / L, thiadizuron 0.5mg / L, then add 30g / L sucrose, adjust the pH to 5.9 with acid or alkali, add 7g / L agar and heat to dissolve, then pack in the Erlenmeyer flask 121 After sterilizing at ℃, cool to room temperature as basic medium;

[0019] 2. The saffron corm is sterilized by 70% ethanol for 0.5 minutes, then sterilized with 2% active chlorine sodium hypochlorite solution for 10 minutes, washed three times with sterile water, and cut into 1cm 2 as explants on the above medium at 23°C and cultured in the dark for 4 weeks, a light yellow callus can be induced;

[0020] 3. The pale yellow callus was added with 0.25 mg / L of 2,4-dichlorophenoxyacetic acid and N 6 -Benzyl adenine 2mg / L MS basic medium 23 ℃ dark culture for 3-6 weeks can be partially transformed into white tumor-like callus, the white tumor-like callus is picked out, after adding naphthaleneacetic acid 2mg / L, N 6 -Benzyladenine 2mg / L, t...

Embodiment 2

[0024] 1. Add naphthaleneacetic acid 1mg / L, N 6 - 2 mg / L benzyl adenine and 0.5 mg / L thiadizuron, then add 30 g / L sucrose, adjust the pH to 5.9 with acid or alkali, add 7 g / L agar and heat to dissolve, then pack in a Erlenmeyer flask at 121°C After disinfection, cool to room temperature as basic medium;

[0025] 2. The saffron corm is sterilized by 70% ethanol for 0.5 minutes, then sterilized with 2% active chlorine sodium hypochlorite solution for 10 minutes, washed three times with sterile water, and cut into 1cm 2 As explants, the cut pieces were cultured on the above medium at 23°C, 2000lux, 12h / d light conditions for 4 weeks, and white tumor-like tissues could be induced;

[0026] 3. The tumor-like tissue was cultured and proliferated in the dark at 23°C on 1 / 2 MS basic medium supplemented with 0.5 mg / L naphthaleneacetic acid, 1 mg / L kinetin, 0.5 mg / L thiadizuron and 300 mg / L acid hydrolyzed casein;

[0027] 4. The white tumor-like material was cultured in the dark at 2...

Embodiment 3

[0030] 1. The white nodular tissue that obtains as embodiment two, add 0.2mg / L of naphthaleneacetic acid, zeatin 2mg / L, thiadizuron 0.5mg / L and 300mg / L on the B5 culture medium of acid hydrolyzed casein 21 ℃ Proliferation in dark culture;

[0031] 2. The proliferating tumorous tissue is transferred to a periodic immersion reactor and an atomization reactor, the inoculation amount is 50g / L, and the medium is changed to indole-3-acetic acid 0.5mg / L, N 6 - 1 / 2MS medium of benzyl adenine 0.5mg / L, 300mg / L acid hydrolyzed casein, 18℃ light 12h / d, 2000lux culture, periodic immersion reactor immersion cycle 5min / 2h, ventilation 0.1vvm, mist The atomization cycle of the chemical reactor is 10min / 2h, the ventilation rate is 0.1vvm, and corms are formed in 6-8 weeks, and the induction rate is 22% and 28%. Step 5, the step 4 method of example two are cultivated to produce more bulbs;

[0032] 3. After the bulbs or root seedlings are hardened under natural conditions indoors for a week, ...

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Abstract

A large-scale tissue culture method for fast reproducing the seedling or seed bulb of saffron includes such steps as inducing different kinds of calli, and culturing them in relative bioreactors in large scale to obtain a great deal of the seedlings or seed bulbs of saffron.

Description

technical field [0001] The invention belongs to the field of biochemical engineering, and relates to a method for large-scale tissue culture and rapid propagation of saffron bulbs (seedlings). Background technique [0002] Saffron (Crocus sativus L), also known as saffron and saffron, is a perennial herb of the genus Crocus (Crocus L.) in the family Iridaceae. [0003] Saffron is native to Spain, Greece and other southern European countries, as well as Iran, India and other places, and is mainly used as natural edible spices and seasonings as well as cosmetic and beauty products. Taking its stigma as medicine has the effects of invigorating blood circulation, removing blood stasis, rejuvenating, analgesic, invigorating the stomach, and stimulating the menstrual flow. It has a long history of medicinal use at home and abroad. In recent years, with the research on the active components of saffron, it has been found that the components of saffron, crocetin, saffron aldehyde, c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 赵兵陈文浩王玉春王晓东
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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