Method for producing 1,3-propylene of using glycerol anaerobic fermentation

An anaerobic fermentation and propylene glycol technology, applied in 1 field, can solve the problems of low fermentation conversion rate, achieve the effects of reducing production cost, shortening fermentation time, and improving substrate conversion rate

Active Publication Date: 2007-05-02
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0008] Aiming at the low conversion rate of glycerol anaerobic fermentation by a single bacterium, the present invention provides an improved method for synthesizing 1,3-propanediol by glycerol fermentation, that is, adding an appropriate amount of organic intermediate metabolism in the fermentation medium or anaerobic fermentation process Molecules, strengthen the subsequent metabolic process of pyruvate in the oxidative metabolic branch, so that the subsequent metabolic flow of

Method used

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Embodiment 1

[0023] The bacterial strain used in the embodiment of the present invention is Klebsiella pneumoniae (Klebsiella pneumoniae), comes from the patent strain of Sinopec Fushun Petrochemical Research Institute, and the bacterial strain is in China General Microorganism Culture Collection Center (CGMCC), and the bacterial strain preservation number : 0798.

[0024] There are two types of medium: seed medium and fermentation medium:

[0025] Seed medium composition: (1L)

[0026] K2 HPO 4 : 34g KH 2 PO 4 : 13g

[0027] (NH) 4 SO 4 : 6g MgSO 4 ·7H 2 O: 0.2g

[0028] CaCl 2 ·H 2 O: 0.02g CaCO 3 : 2.0g

[0029] Yeast extract: 1.0g Glycerin: 60g

[0030] Fe 2+ Solution: 2.0mL Trace element solution I: 1.0mL

[0031] where Fe 2+ Solution: (FeSO 4 5g / L HCl (37%) 4.0mL)

[0032] Composition of trace element solution I (mg / L):

[0033] MgSO 4 4H 2 O: 100mg / L ZnCl 2 : 70mg / L

[0034] Na 2 MoO 4 2H 2 O: 35mg / LH 3 BO 3 : 60mg / L

[0035] CoCl 2 ·6H 2 O: 200mg / L ...

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Abstract

This invention provides a promoting cell synthesizing trimethylene glycol method by adding organic intermediary metabolism molecule, and it belongs to trimethylene glycol biosynthesize technology field. Aim at the problem of low transformation efficiency of single cell using glycerine anaerobic fermentation in present technology, this invention realizes ideal flux distribution of substrate and product during the process of trimethylene glycol fermentation, which makes high effect trimethylene glycol fermentation into reality. The invention breaks through the bottleneck of low trimethylene glycol transformation efficiency using glycerine biosynthesis, which improves the transformation efficiency of substrate, shortens fermenting time and cuts down the cost.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for bioconverting a fermentable glycerin carbon source into 1,3-propanediol by using a single microorganism. Background technique [0002] As we all know, 1,3-propanediol is an important chemical raw material and pharmaceutical intermediate, and it is a monomer with potential application value in the production of polyester fiber and in the production of polyurethane and cyclic compounds. [0003] At present, the industry mainly adopts chemical synthesis to produce 1,3-propanediol. It is known that there are many kinds of chemical ways to produce 1,3-propanediol, but there are many by-products in the chemical method, the separation and purification of the product are difficult, and the production cost is correspondingly high. In contrast, the production of 1,3-propanediol by biotransformation has attracted more and more attention because of its characteristics of u...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/22
Inventor 王领民金平佟明友
Owner CHINA PETROLEUM & CHEM CORP
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