Process for extracting beta-mannanase utilizing Erwinia

Abstract

This invention involves the technology of extraction of beta-mannan enzyme from Erwinia bacilli in the industry of biological agents. The technical program includes original species activation, primary seed cultivation, secondary seed cultivation, fermentation, proteinase inhibitor, crude separation, refining of concentrated efficient bacilli and mannan-liquid, formulas, vacuum packaging technology and condensed efficient liquid agent and mannan enzyme products. This invention uses centralized preparation agent program to reduce technical difficulty of bio-degumming and biological pulping process, and can reduce the production cost by extracting cell pellets and secretions from the same broth. The efficient concentrated liquid agent, produced by this invention technical programs, is suitable for bio-degumming of crudefiber plants, biological pulping of grasses and biological glycosylated processing of herbaceous fiber raw materials biopulping alcohol; mannan enzymes are widely used in food, medicine, fodder, paper, textiles, petroleum mining industry.

Description

1. Technical field [0001] The invention relates to a process for simultaneously preparing bacterial agents and enzyme preparations from the same fermented liquid through diversion. Bacteria agents are mainly used for the extraction and refining of herbal fibers, including hemp biodegumming in the textile industry, biopulping in the paper industry, and biosaccharification of herbal fiber raw materials in the alcohol production industry; β-mannanase is used in food, medicine, and feed , papermaking, textile printing and dyeing, petroleum exploration and biotechnology research have been widely used. 2. Background technology [0002] (1) Development status and trends at home and abroad [0003] 1. The development of herbal fiber resources is a global development trend [0004] With the shortage of oil and forest resources and the improvement of the quality of human life, the domestic and foreign textile and paper industries are developing an upsurge in the development and util...

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Problems solved by technology

[0029] The research on the breeding of mannanase high-yielding strains at home and abroad started relatively late and achieved relatively little results. Therefore, although the market prospect of mannanase is very good, the industrial development is relatively slow due to the relatively high processing costs.

At the same time, compared with textile and papermaking industries, hemp degumming and grass pulping are only a preliminary process of extracting fibers from herbal fiber raw materials. If the production cost of high-efficiency bacterial agents is too high, it will inevitably lead to a lack of market price for the final product. Competitiveness in terms of

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