Anti-plasmodium compositions and methods of use

a technology of compositions and anti-plasmodium, applied in the field of microbiology and immunology, can solve problems such as coma, rapid death, and convulsions, and achieve the effects of preventing coma, rapid death, and preventing coma

Inactive Publication Date: 2002-09-12
HEALTH & HUMAN SERVICES DEPT OF THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

0010] The present invention provides compositions and methods for detecting, diagnosing, preventing and treating Plasmodium falciparum and Plasmodium falciparum related infections. In particular, the compositions include a family of merozoite ligands that are erythrocyte binding proteins (EBPs). The EBPs of the invention are paralogues of EBA-175, an EBP in P. falciparum. The invention further comprises antibodies specific to each paralogue, peptides of the paralogues, and peptides of the antibodies that specifically inhibit binding of P. falciparum erythrocyte binding proteins to erythrocytes.

Problems solved by technology

Additionally, when the central nervous system is involved, symptoms include delirium, convulsions, paralysis, coma, and even rapid death.

Method used

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  • Anti-plasmodium compositions and methods of use
  • Anti-plasmodium compositions and methods of use
  • Anti-plasmodium compositions and methods of use

Examples

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example 1

Materials and Methods for Detecting P. falciparum EBPs

[0098] Genomic Database

[0099] The sequence data for P. falciparum chromosome 13 was obtained from The Sanger Centre website at http: / / www.sanger.ac.uk / Projects / P_falciparu- m / . Sequencing of P. falciparum chromosome 13 was accomplished as part of the Malaria Genome Project with support by The Wellcome Trust.

[0100] Parasites

[0101] Plasmodium falciparum 3D7 strain (clone of NF54, Amsterdam Airport, human challenge strain) and FVO strain (Aotus adapted) were maintained as previously reported (Vemes et al., 1984). When appropriate, schizonts were purified on Percoll density gradient. The 3D7 parasites were metabolically labeled with TRAN.sup.35S-LABEL.TM. (ICN Radiochemicals, Irvine, Calif.) as previously described (Sim et al., 1994b). Essentially 2.times.10.sup.8 parasites in 10 ml RPMI-1640 culture media deficient in methionine and cysteine were incubated with 1 mCi TRAN.sup.35S-LABEL.TM. for 4 hours for parasite-cell pellets and 1...

example 2

Expression of EBP2 in Parasitized Erythrocytes

[0115] To examine whether ebp2 was transcribed a mRNA transcript was detected with RT-PCR. Parasite mRNA isolated from 3D7 schizont-infected erythrocytes was used for first strand synthesis with random primers or with a poly dT primer and then PCR amplified with an ebp2 primer pair or eba-175 primer pair as a control. Appropriate size DNA fragments were detected on an ethidium bromide stained agarose gel for ebp2 and eba-175. Using genomic 3D7 DNA, ebp2 RII was amplified by PCR and cloned into a naked DNA vaccine plasmid (Hartikka et al., 1996). Both the forward and reverse strands of the DNA fragment were sequenced. A single point nucleotide change at position 1654 (A to G) encoded an amino acid substitution from Asn to Asp. This single amino acid difference is the result of a PCR-introduced artifact.

[0116] Recombinant EBP2 RII and EBA-175 RII derived from supernatants by transient transfection of UM449 cells were immunoblotted with spe...

example 3

EBP2 RII Specific Antibodies Recognize a 130 kDa P. falciparum Protein

[0119] To determine the molecular mass of EBP2 [.sup.35S]-metabolically labeled P. falciparum 3D7 strain schizont-infected erythrocyte lysates were incubated with EBP2 RII specific antibodies coupled to Protein G-sepharose. [.sup.35S]-labeled P. falciparum schizont-infected erythrocyte lysate were immunoprecipitated and human erythrocyte bound [.sup.35S]-labeled EBP2 were detected from [.sup.35S]-labeled parasite culture supernatants. EBA-175 specific polyclonal rabbit IgG was included as a control. Results were obtained with EBP2 specific polyclonal sera or control and EBA-175 specific polyclonal IgG and control. [.sup.35S]-labeled EBP2 and EBA-175 were immunoprecipitated from lysates of erythrocytes with bound EBPs. [.sup.35S]-labeled EBP2 and EBA-175 were eluted off human erythrocytes with 1M NaCl and then immunoprecipitated with EBP2 or EBA-175 specific antibodies. Mouse and rabbit adjuvant controls and molecu...

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Abstract

Compositions that inhibit the binding of Plasmodium falciparum to erythrocytes include a family of erythrocyte binding proteins (EBPs). The EBPs are paralogues of the P. falciparum binding protein EBA-175. The present invention includes peptides of the paralogues that prevent the binding of P. falciparum. Antibodies specific for each paralogue that also prevent the binding of P. falciparum are also included. Methods of the invention utilize the paralogues, antibodies thereof and peptide compositions for the diagnosis, prevention, and treatment of P. falciparum diseases such as malaria, as well as methods for the detection of P. falciparum in biological samples and culture media.

Description

FIELD OF THE INVENTION[0001] The present invention relates to the fields of microbiology and immunology and more specifically relates to compositions and methods for the detection, diagnosis, prevention and treatment of malaria. In particular, the invention pertains to a family of paralogues of EBA-175, antibodies specific to each paralogue, peptides of the paralogues and peptides of the antibodies that inhibit the binding of Plasmodium falciparum erythrocyte binding protein antigens to erythrocytes.BACKGROUND OF THE INVENTION[0002] Although endemic malaria has disappeared from the United States, malaria continues to be one of the most important infectious diseases in the world as it kills millions of people each year in countries throughout Africa, Asia and Latin America. The characteristic presentation of malaria is chills followed by a fever ranging from 104-107.degree. F., followed by profuse sweating. Other manifestations of malaria include anemia, decreased blood flow to vital...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00A61P33/02A61P33/06C07K14/445C12N15/12
CPCA61K39/00Y02A50/412C07K14/445A61P33/02A61P33/06Y02A50/30
Inventor NARUM, DAVID L.SIM, KIM L.
Owner HEALTH & HUMAN SERVICES DEPT OF THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC
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