Biocontrol for plants with bacillus subtilis, pseudomonas putida, and sporobolomyces roseus

a technology of sporobolomyces roseus and plants, applied in the field of biocontrol of plants with bacillus subtilis, pseudomonas putida, and sporobolomyces roseus, can solve the problems of difficult measurement accuracy, lack of suitable techniques for assaying soil samples, and large population size of many soil microorganisms, etc., to achieve the effect of enhancing the growth of treated plants

Inactive Publication Date: 2005-11-24
BERGSTROM GARY C +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0018] The biocontrol agents of the present invention are highly useful in agriculture to protect plants from a variety of plant bacterial, fungal, and viral diseases. In addition, these agents can enhance the growth of treated plants. Significantly, these effects are achieved without being hazardous to animals or humans.

Problems solved by technology

However, most biocontrol agents have multiple pathogen and crop uses.
One barrier to acquiring an understanding of soil microbial systems has been the lack of suitable techniques for assaying soil samples.
Population sizes of many soil microbes, especially fungi, are difficult to measure accurately for several reasons.
In addition, many microbes are not easily cultured on standard media, although they may play significant roles in disease suppression, as with the mycoparsite Sporidesmium sclerotivorum for control of lettuce drop (Adams et al., “Economical Biological Control of Sclerotinia Lettuce Drop by Sporidesmium sclerotivorum,” Phytopathology, 80:1120-24 (1990)).
All of these shortcomings are compounded by the difficulty of sampling, particularly sampling of microsites.
Suitable systems also need to be developed for production, formulation and delivery of biocontrol agents, because these processes can greatly affect efficacy of the biocontrol agent.

Method used

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  • Biocontrol for plants with bacillus subtilis, pseudomonas putida, and sporobolomyces roseus
  • Biocontrol for plants with bacillus subtilis, pseudomonas putida, and sporobolomyces roseus
  • Biocontrol for plants with bacillus subtilis, pseudomonas putida, and sporobolomyces roseus

Examples

Experimental program
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Effect test

example 1

Characterization of the Bacillus Subtilis Isolate TrigoCor 1448 (ATCC Accession No. 202152)

[0038] The bacterial isolate deposited as ATCC Accession No. 202152 (also referred to as “TrigoCor 1448”), was originally identified as Paenibacillus macerans (based on GC-FAME analyses and a database search provided by Microbe Inotech Laboratories, St. Louis, Mo.). However, in February 2001, TrigoCor 1448 was reclassified as Bacillus subtilis, based on a Basic Local Alignment Search Tool (“BLAST”) search of sequences of the 16S ribosomal RNA (rRNA) gene. A 500 base pair segment of the 16S rRNA of TrigoCor 1448 was sequenced by Microbe Inotech Laboratories (St. Louis, Mo.) and has been identified as Bacillus subtilis with 99 percent confidence, based on the sequence similarity with entries in the NCBI's GeneBank nucleotide database. Based on this analysis, 529 out of 532 base pairs of the 16S rRNA gene of TrigoCor 1448 matched those of Bacillus subtilis strain TB11 (used for production of a b...

example 2

Ribotyping Analysis of Bacillus Subtilis Isolate TrigoCor 1448

[0039] TrigoCor 1448 was further characterized based on its ribotype. Ribotyping is a diagnostic method used to identify and classify bacteria based on differences in their rRNA genes. This method generates a precise and highly reproducible rRNA “fingerprint” that can be used to classify bacteria and distinguish them beyond the species level. In general, this method involves extracting DNA from a sample bacterial colony of interest and then restricting the DNA into discrete-sized fragments. Thereafter, the DNA is transferred to a membrane and probed with a region of the rRNA operon to reveal the pattern of rRNA genes for the sample. The pattern, also referred to as a ribotype or riboprint pattern, is then recorded, digitized, and stored in a database. The sample's riboprint pattern may then be compared against those of other bacteria. Variations that exist among bacteria in both the position and intensity of the resultin...

example 3

In-Vitro Assays for Antibiosis of F. Graminearum by Bioprotectants

[0044] In paired treatments, the radial growth (mm) of F. graminearum in the presence or absence of Bacillus subtilis isolated TrigoCor 1448 (ATCC Accession No. 202152), Pseudomonas putida (ATCC Accession No. 202153), or Sporobolomyces roseus (ATCC Accession No. 74456) was measured as a means of ascertaining the antibiosis of the pathogen by candidate bioprotectants. Each bacterial or yeast isolate was transferred onto ¼ strength potato dextrose agar (i.e. PDA) in a circular pattern by means of a small sterile glass funnel. After 2 days of incubation at ambient temperature, an agar disk containing mycelia of the pathogen was transferred into the center of the ring-shaped colony of the bioprotectant or, in the control treatment, onto an uninoculated media plate. The radial growth of the pathogen was measured after 5 days of incubation. There was a minimum of four replicates per treatment.

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Abstract

The present invention is directed to isolated Bacillus subtilis, Pseudomonas putida, and Sporobolomyces roseus which are useful as a biocontrol agent. These organisms are useful in a method of imparting to plants protection against plant pathogens by applying them to plants, plant seeds, or soil surrounding plants under conditions effective to impart disease protection to the plants or plants produced from the plant seeds. The biocontrol agents are also useful in a method of enhancing plant growth which involves applying them to plants, plants seeds, or soil surrounding plants under conditions effective to enhance growth in the plants or plants produced from the plant seeds.

Description

[0001] This application claims benefit of U.S. Provisional Patent Application Ser. No. 60 / 053,310, filed Jul. 22, 1997, and is a continuation-in-part of U.S. patent application Ser. No. 09 / 118,656, filed Jul. 17, 1998.[0002] The present invention was made with support under USDA Hatch Project No. NYC153-472. The U.S. Government may have certain rights.FIELD OF THE INVENTION [0003] The present invention relates to biocontrol for plants with Bacillus subtilis, Pseudomonas putida, and Sporobolomyces roseus. BACKGROUND OF THE INVENTION [0004] There are approximately 40 biocontrol products commercially available for the control of plant diseases worldwide. Biocontrol products are available to control many diverse pathogens, as recently reviewed by Fravel, et al., “Availability and Application of Biocontrol Products,”Biological and Culture Tests for Control of Plant Diseases, 11:1-7 (1996). At least 27 genera of fingi, 3 genera of bacteria, and 4 genera of nematodes are targeted for contr...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01N63/22A01N63/27A01N63/32A61K35/68
CPCA01N63/00A01N63/04C12R1/125C12R1/40C12R1/645Y10S435/839Y10S435/832A01N2300/00A61K39/07C12N1/20A61K2039/58C12N1/14A01N63/27A01N63/22A01N63/32C12R2001/125C12N1/205C12R2001/40C12R2001/645C12N1/145
Inventor BERGSTROM, GARY C.CORIO DA LUZ, WILMAR
Owner BERGSTROM GARY C
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