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Apparatus for monitoring bioluminescence of biological samples

a bioluminescence and apparatus technology, applied in the field of apparatus for monitoring the bioluminescence of biological samples, can solve the problems of difficult comparison and investigation of data every experience, the inability to conduct functional analysis of all genes, and the need to destroy a cell in these methods

Inactive Publication Date: 2006-03-16
NATIONAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014] It is, therefore, an object of the invention to provide an apparatus for monitoring a bioluminescence of an organism sample which can monitor the luminescence in a high reliability while growing the organism samples under the uniform conditions.
[0023] In the other preferable embodiment of the invention, said sensor precisely determines a relative position between a photoelectron amplifier tube as a bioluminescence detector in the monitoring part and said plate.
[0029] The apparatus for monitoring the luminescence of the organism sample according to the invention develops an advantageous effect that the organism samples can be monitored within a wide temperature range because the monitoring part is separated from a part being weak to heat.
[0030] Further, the apparatus for monitoring the luminescence of the organism sample according to the invention develops an advantageous effect that the bioluminescence of the plant can be monitored uniformly. That is, according to the invention, the reliable monitored result can be provided because the condition for culturing the organism sample on the plate can be set uniformly.
[0031] Also, the apparatus for monitoring the luminescence of the organism sample according to the invention develops an advantageous effect that the apparatus can be miniaturized as compared with the conventional apparatus.

Problems solved by technology

In these methods, however, it is impossible to conduct functional analysis of all genes because the detection sensitivity is low.
Also, it is required to destroy a cell in these methods because an experiment is carried out by using mRNA or protein as a material.
In the above method using the scintillation counter, however, it has been confirmed that an amount of light irradiated to each plate and an amount of light irradiated into each well within the plate are very non-uniform and the cultivation becomes non-uniform and hence a large scattering is caused in the monitored result.
Further, it has been confirmed that the comparison and investigation of data every experience are not easy because the monitoring number per unit time changes as the number of the plates to be tested changes.
Up to now, however, there is not developed an apparatus utilizing the merits of the real-time method to the utmost to cope with the large-scale monitoring.
Although the scintillation counter is generally used in the monitoring of the luminescence of organism samples, it is difficult to monitor plant-based organism samples under uniform growth cultivating conditions (especially, the light condition) because the light is required for the growth.
In the method of combining the sample cultivating and transferring apparatus and the scintillation counter, a large space is required for setting the apparatus and the cost becomes high.

Method used

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  • Apparatus for monitoring bioluminescence of biological samples
  • Apparatus for monitoring bioluminescence of biological samples
  • Apparatus for monitoring bioluminescence of biological samples

Examples

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example 2

[0074] Next, there is examined an influence of a temperature on the monitoring sensitivity. As a standard light source is used CXS-2011 made by Aloka Co., Ltd. After the standard light source is placed in the well of the plate, a counting value of the well is monitored under temperature environments of 15, 20, 25, 30, 35, 40, 45 and 50° C.

[0075] The results are as follows. In order to clarify what temperature range can be used in the apparatus for monitoring the luminescence according to the invention, the luminescence is monitored under various temperature conditions about the main body of the apparatus (the culture part, the convey part and the monitoring part) (FIG. 12). When the monitoring temperature is raised from 15° C. to 50° C. every 5° C., the counting value of the background increases from 3 cps to 90 cps. On the other hand, the counting value of the standard light source is constant at 2.0×105 cps. As seen from this result, the monitoring of the luminescence in the appa...

example 3

[0076] The monitoring of a bioluminescence reporter strain of Arabidopsis is carried out below. As a sample for monitoring the bioluminescence is used a bioluminescence reporter strain CAB2::LUC of Arabidopsis. (Millar et al., 1992, Plant Cell Vol. 4, p 1075-1087). This stain is a stain wherein a luminescence reporter gene connected a region for encoding luciferase gene LUC of a firefly down the stream of the promoter region of CAB2 gene of Arabidopsis encoring a chlorophyll a / b binding protein is transfused to a genome of a wild type strain C24, and shows a bioluminescence rhythms with period lengths of about 24 hours under constant light condition by being controlled with a circadian clock. Also, the strain shows a decrease of an amount of the bioluminescence and an elongation of the period of the rhythms of the bioluminescence when a light intensity irradiated during culture is decreased. (Millar et al., 1992, Plant Cell Vol. 4, p 1075-1087; Somers et al., 1998, Development Vol. ...

example 4

[0081] The monitoring of the luminescence is carried out by using a luminescence reporter strain of thermophilic blue-green bacterium.

[0082] A psbA1 luminescence reporter strain of thermosynechococcus elongates is used. This strain is a strain wherein a luminescence reporter gene connected to the luciferase gene operon (X1 luxAB) from Xenorhabdus luminescence belonging to thermoduric bacteria at the downstream of the promoter sequence of psbA1 gene of T. elongates is gene-transfused to the genome of the wild type. The rhythm of the bioluminescence can be observed by adding a luminous substrate to psbA1 luminescence reporter strain because the transcription activity of psbA1 gene varies in a daily periodicity and shows a circadian rhythm. A colony is formed by cultivating a cell of psbA1 luminescence reporter strain on BG-11 solid medium at 52° C. under a continuous irradiation of a white light of 50 μmol m−2 s−1. In each well of the plate is poured 20 μl of BG-11 liquid medium, and...

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Abstract

An apparatus of monitoring the bioluminescence of organism samples comprises a culture part including a plurality of plates for putting plural organism samples and capable of cultivating said plural organism samples, a monitoring part for monitoring bioluminescences of said plural organism samples, a convey means for conveying said plural plates to said monitoring part, a control part for controlling a movement of said convey means and controlling a monitoring condition of the bioluminescence of said organism sample, and a computer for record and analysis, wherein said monitoring part, control part and computer are separated from each other.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] This invention relates to an apparatus for monitoring a bioluminescence of a biological sample, and especially relates to an apparatus for monitoring a bioluminescence of a biological sample wherein a monitoring part and a control part are separated from each other. [0003] 2. Description of Related Art [0004] In various organisms, all genome sequences are successively determined, and the importance on a cyclopaedically functional analysis of a genome based on genome information is increasing. As an effective method of the genome functional analysis are used a DNA array method and a proteomix method. In these methods, however, it is impossible to conduct functional analysis of all genes because the detection sensitivity is low. Also, it is required to destroy a cell in these methods because an experiment is carried out by using mRNA or protein as a material. [0005] A method of monitoring a bioluminescence in real tim...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00C12M1/38C12M3/00G01N21/13G01N21/25G01N21/76G01N35/00G01N35/02G01N35/04
CPCG01N21/13G01N21/253C12M41/36G01N35/028G01N2035/0422G01N21/763C12M23/12
Inventor ISHIURA, MASAHIROOKAMOTO, KAZUHISAONAI, KIYOSHIFURUSAWA, TAKAYOSHI
Owner NATIONAL UNIVERSITY
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