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AMPK activating agent

a technology of activating agent and ampk, which is applied in the direction of biocide, drug composition, animal husbandry, etc., can solve the problems of increasing the number of people, grave social problems, and diabetes associated with obesity

Inactive Publication Date: 2007-03-08
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, population with obesity has increased, and so-called lifestyle-related diseases such as diabetes associated with obesity have become grave social problems.
As a result, so-called lifestyle-related diseases such as diabetes have been growing in number with an increase in population with obesity and become grave social problems.
However, it is quite difficult to perform routine exercise in real life.
However, most of their mechanisms of action on the improvement of obesity remain unknown.
Moreover, their safety is not sufficiently confirmed owing to poor history of use in foods.
Thus, the fact is that their usefulness is not sufficiently established.
However, easily available raw materials with rich history of use in foods, high safety, and excellent workability were very few until now.
In recent years, reduced endurance attributed to lack of exercise has been a problem along with the rapid aging of the population, the sophistication of transportation systems, and the progression of information / communication technology.
However, the fact is that many persons cannot make enough time for exercise due to busyness or can not do exercise due to physical problems even if they want to do so.
However, its physiological activity has hardly been reported so far.
Moreover, the influence of nootkatone on exercise capacity including endurance or on fatigue has never been known so far.

Method used

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  • AMPK activating agent

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0032] A muscle cell line (C2C12) was used to evaluate the AMPK-activating action of nootkatone according to the procedures below with AMPKα and AMPKβ phosphorylation as indexes.

[0033] Cells of the murine muscle cell line (C2C12) were seeded onto a 25 cm2 flask and cultured in DMEM (+10% FBS, +antimicrobial agent) at 37° C. for 1 to 2 days. When the cells became confluent, the culture solution was removed, and the cells were washed with PBS (−) and further cultured for 7 to 8 days in fresh DMEM (2% (V / V) horse serum), which was in turn replaced by a fresh culture solution every 2 to 3 days. The culture solution was then removed, and the cells were washed with PBS (−) and further cultured in fresh DMEM (−FBS) for 1 day. After the removal of the culture solution, the cells were cultured in DMEM (−FBS) containing a predetermined concentration of nootkatone (obtained from Wako Pure Chemical Industries, Ltd.) for 60 minutes. After the culture solution was subsequently removed and the ce...

example 2

Effect of Nootkatone

[0037] The antiobesity, antidiabetic, and antilifestyle-related disease effects of nootkatone were evaluated as described below.

[0038] Seven-week-old C57BL / 6 male mice were divided into 3 groups each containing 10 mice and raised with each diet of the composition described in Table 2.

TABLE 2(%)Group 1Group 2Group 3Plant oil52525Lard055Sucrose01313Casein202020Potato starch66.528.528Vitamin111Mineral3.53.53.5Cellulose444Nootkatone000.5

[0039] Twenty-two weeks later, their body weights were measured, while blood was collected from the mice under ether anesthesia and under non-fasting conditions to measure serum glucose, cholesterol, triglyceride, insulin, and leptin levels. The amount of visceral fat (epididymal fat, retroperitoneal fat, and perirenal fat) was also measured. The result is shown in Table 3.

TABLE 3Group 1Group 2Group 3Body weight (g)33.240.330.4Epididymal fat (g)0.891.910.47Retroperitoneal fat (g)0.270.550.15Perirenal fat (g)0.150.380.07

[0040] A ...

example 3

Lipid Metabolism-Activating Effect

[0046] Seven-week-old Balb / c male mice were divided into 2 groups, to which a physiological saline (control) or nootkatone at 200 mg / kg of body weight was then orally administered for 10 consecutive days. Then, their livers and skeletal muscles (gastrocnemius muscle+soleus muscle) were collected. The livers and skeletal muscles were respectively homogenized in a buffer (250 mM sucrose, 1 mM EDTA in 10 mM HEPES (pH 7.2)), and the insoluble tissue residues were removed by centrifugation to obtain supernatants. The obtained supernatants were measured for protein levels. The protein levels were adjusted among samples to constant levels, and the samples were used in the measurement of lipid metabolism activity (β-oxidation activity). A 100-μg aliquot of the supernatant protein was reacted at 37° C. for 20 minutes with 0.1 μCi [14C]-palmitic acid in a buffer (50 mM Tris-HCl (pH 8.0), 40 mM NaCl, 2 mM KCl, 2 mM MgCl2, 1 mM DTT, 5 mM ATP, 0.2 mM L-carnitin...

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Abstract

The present invention relates to provision of AMPK activating agent, endurance enhancing agent, and antifatigue agent, which have rich history of use in foods, high safety, readily available, and high workability, a method of activating AMPK, and a method of preventing, treating and / or ameliorating a life-style-related disease. The present invention provides AMPK activating agent, endurance enhancing agent, and antifatigue agent, each of which contains nootkatone as an active agent. The present invention provides a method of activating AMPK characterized by administering nootkatone in an effective amount, and a method of preventing, treating and / or ameliorating a life-style-related disease characterized by administering nootkatone in an effective amount.

Description

BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The present invention relates to an AMPK (AMP-activated protein kinase) activating agent. [0003] 2. Background of the Invention [0004] In recent years, population with obesity has increased, and so-called lifestyle-related diseases such as diabetes associated with obesity have become grave social problems. Obesity is generated as a result of energy excess in which energy intakes exceed energy consumption. Thus, the bases for prevention and improvement of obesity and diabetes developed therewith are, for example, a decrease in energy intakes by decreasing meal size or an increase in energy consumption by exercise. [0005] However, in the modern society, lipid intakes have increased due to the westernization of eating habits, while the amount of exercise and energy consumption have decreased due to the proliferation of automobiles, and soon. As a result, so-called lifestyle-related diseases such as diabetes have been g...

Claims

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Application Information

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IPC IPC(8): A61K31/122
CPCA61K31/122A61P43/00
Inventor MURASE, TAKATOSHIMINEGISHI, YOSHIHIKO
Owner KAO CORP
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