Modulating plant oil levels

a technology of plant oil and level, applied in the field of moduleating plant oil levels, can solve the problems of inability to detect the introduction of nucleic acid in daughter cells, and consuming too much saturated fa

Active Publication Date: 2009-12-31
CERES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0013]This document provides methods and materials related to plants having modulated (e.g., increased or decreased) levels of oil. For example, this document provides transgenic plants and plant cells having increased levels of oil, nucleic acids used to generate transgenic plants and plant cells having increased levels of oil, and methods for making plants and plant cells having increased levels of oil. Such plants and plant cells can be grown to produce, for example, seeds having increased oil content. Increasing the oil content of seeds can increase the nutritional value of the seeds and the yield of oil obtained from the seeds, which may benefit both food consumers and producers.

Problems solved by technology

Eating too much saturated fat is one of the major risk factors for heart disease.
Transiently transformed cells typically lose all or some portion of the introduced nucleic acid construct with each cell division such that the introduced nucleic acid cannot be detected in daughter cells after a sufficient number of cell divisions.

Method used

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Examples

Experimental program
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example 1

Transgenic Plants

[0312]The following symbols are used in the Examples: T1: first generation transformant; T2: second generation, progeny of self-pollinated T1 plants; T3: third generation, progeny of self-pollinated T2 plants; T4: fourth generation, progeny of self-pollinated T3 plants. Independent transformations are referred to as events.

[0313]The following is a list of nucleic acids that were isolated from Arabidopsis thaliana plants. Ceres Clone 25429 (At3g44590; SEQ ID NO:93) is a cDNA clone that is predicted to encode a 113 amino acid (SEQ ID NO:94) ribosomal polypeptide. Ceres Clone 41573 (At5g15140; SEQ ID NO:80) is a cDNA clone that is predicted to encode a 298 amino acid (SEQ ID NO:81) aldose 1-epimerase polypeptide. Ceres Clone 5750 (At2g27960; SEQ ID NO:110) is a cDNA clone that is predicted to encode an 87 amino acid (SEQ ID NO:111) cyclin-dependent kinase regulatory subunit polypeptide. Ceres Clone 121021 (SEQ ID NO:151) is a DNA clone that is predicted to encode a 199...

example 2

Analysis of Oil Content in Transgenic Arabidopsis Seeds

[0318]An analytical method based on Fourier transform near-infrared (FT-NIR) spectroscopy was developed, validated, and used to perform a high-throughput screen of transgenic seed lines for alterations in seed oil content. To calibrate the FT-NIR spectroscopy method, a sub-population of transgenic seed lines was randomly selected and analyzed for oil content using a direct primary method. Fatty acid methyl ester (FAME) analysis by gas chromatography-mass spectroscopy (GC-MS) was used as the direct primary method to determine the total fatty acid content for each seed line and produce the FT-NIR spectroscopy calibration curves for oil.

[0319]To analyze seed oil content using GC-MS, seed tissue was homogenized in liquid nitrogen using a mortar and pestle to create a powder. The tissue was weighed, and 5.0±0.25 mg were transferred into a 2 mL Eppendorf tube. The exact weight of each sample was recorded. One mL of 2.5% H2SO4 (v / v in ...

example 3

Analysis of Protein Content in Transgenic Arabidopsis Seeds

[0326]An analytical method based on Fourier transform near-infrared (FT-NIR) spectroscopy was developed, validated, and used to perform a high-throughput screen of transgenic seed lines for alterations in seed protein content. To calibrate the FT-NIR spectroscopy method, total nitrogen elemental analysis was used as a primary method to analyze a sub-population of randomly selected transgenic seed lines. The overall percentage of nitrogen in each sample was determined. Percent nitrogen values were multiplied by a conversion factor to obtain percent total protein values. A conversion factor of 5.30 was selected based on data for cotton, sunflower, safflower, and sesame seed (Rhee, K. C., Determination of Total Nitrogen In Handbook of Food Analytical Chemistry—Water, Proteins, Enzymes, Lipids, and Carbohydrates (R. Wrolstad et al., ed.), John Wiley and Sons, Inc., p. 105, (2005)). The same seed lines were then analyzed by FT-NI...

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Abstract

Methods and materials for modulating (e.g., increasing or decreasing) oil levels in plants are disclosed. For example, nucleic acids encoding oil-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased oil levels and plant products produced from plants having increased oil levels.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This Application claims priority under 35 U.S.C. 119(e) to U.S. Provisional Application No. 60 / 762,422, filed Jan. 26, 2006, and U.S. Provisional Application No. 60 / 797,077, filed May 1, 2006, incorporated herein by reference in their entirety.BACKGROUND[0002]1. Technical Field[0003]This document relates to methods and materials involved in modulating (e.g., increasing or decreasing) oil levels in plants. For example, this document provides plants having increased oil levels as well as materials and methods for making plants and plant products having increased oil levels.[0004]2. Incorporation-By-Reference & Texts[0005]The material on the accompanying diskette is hereby incorporated by reference into this application. The accompanying compact discs contain one identical file, 11696-204WO1-Sequence.txt, which was created on Jan. 26, 2007. The file named 11696-204WO1-Sequence.txt is 1128 KB. The file can be accessed using Microsoft Word on ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23L1/212A23L1/36A23K1/00C12N5/10C07H21/04C11B1/00C12N15/82A01H5/00A23L7/10A23L19/00A23L25/00
CPCC12N15/8247C07K14/415
Inventor BOBZIN, STEVEN CRAIGMUMENTHALER, DANIELJANKOWSKI, BORISRARANG, JOEL CRUZ
Owner CERES INC
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