Laser-based vaccine adjuvants
a vaccine and laser technology, applied in the field of laser-based vaccine adjuvants, can solve the problems of few chemical or biological adjuvants approved for human use in the west, difficult to develop adjuvants with sufficient efficacy and safety, and aluminum salts
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example 1
Secretion of Hsp70 in Response to the Cu Laser Treatment In Vitro
[0139]Fibroblasts cells derived from mouse embryo were cultured in the Petri dish using the Eagle or No. 199 medium for 24 h. Cell culture was exposed to the light beam of the Cu laser. The levels of HSP70 have been determined before and after irradiation using antibodies to Hsp70 (Russian Patent No. 2242764 7, Aug. 2003. After a 1 minute exposure, the separated supernatant solution displayed 350% increase of the extracellular HSP70, from 3.2 to 11.2 ng / L. The culture cells remained viable after laser irradiation as established by staining with the toluidine blue 0.2%
example 2
Effects of the Cu Laser Irradiation on the Skin In Vivo
[0140]Healthy white mice (CBA, Rappolovo, 50 animals) were treated using Cu laser by exposing a section of an ear to the laser light with a diameter of 5 mm over a period of 1-3 min. After the experiments, the animals were sacrificed at different time points. Immunohistochemical staining using antibodies to HSP70 (Russian Patent No. 2242764, 7, Aug. 2003) of the ventricular epidermal sheets of the ear (FIG. 6) provided a qualitative estimate of the increased levels of the secreted HSP70 as compared to the tissue from untreated ear from the same animal. These data were supported by the results of immunoblotting analysis of the processed ventricular epidermal sheets tissues though these results did not differentiate between secreted and intracellular HSP70. Immunoblotting for HSP70 showed that the combination of the two different wavelengths of light increased the quantity and duration of HSP70 secretion over single wavelengths, a...
example 3
Enhanced Antibody Production in Response to Vaccine Administered with Cu Laser
[0141]In a group of 125 healthy white mice (CBA, Rappolovo), 50 animals received 50 μL of a commercial H3N2 influenza vaccine (VAXIGRIP®, Aventis Pasteur) by subcutaneous injection to the left ear and served as a control group. In an experimental group, 50 mice were administered the same vaccine together with laser treatment (Cu laser at 578 / 511 nm, 1-3 ns pulses at 10 MHz) of the left ear. Of the 50 animals treated with laser, a subgroup of 25 animals was a subject of a one minute exposure whereas another subgroup of 25 mice was a subject of a two-minute exposure. An additional experimental set of 25 mice received the vaccine and exogenous bovine HSP70 (10 μg per injection, dissolved in 50 μL saline solution). The animals were sacrificed and antibody titers were measured 28 days after vaccination following a standard literature protocol [“The immune drugs for grippe prophylactic and diagnostic effectivene...
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