Compositions for use in the treatment of chronic obstructive pulmonary diseases and asthma
a technology for obstructive pulmonary disease and compositions, applied in immunological disorders, instruments, antibody medical ingredients, etc., can solve the problems of limited airflow to and from the lungs, shortness of breath, and inability to reverse the limitation of airflow, so as to prevent the development of chronic obstructive pulmonary disease
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example 1
Anti-Collagen V Antibodies are Increased in COPD Patients
[0182]Plasma was obtained from normal volunteers (non smoking adults, age 18-55), and volunteers with documented emphysema. Levels of anti-colV antibodies in COPD patients and control healthy subjects were detected by the flow cytometry bead assay as described in WO 2007 / 120947.
[0183]Briefly, 1) Streptavidin-coated beads (5 μm, binding capacity 10-20 μg / 1×107 beads (Polyscience, Warrington, Pa.)) were washed two times with sterile PBS. Beads (1×107) were suspended in 100 μl of PBS with 40 μg of human Type V collagen and incubated for 60 minutes at 4° C. 2) A positive control was generated by following the same procedures in 1 above, using 20 μm of rabbit antibody to human collagen V antibody (bioten) (Abeam, Cambridge, Mass.). 3) For each assay, 1×106 conjugated beads were washed two times in PBS, and incubated in 100 μl PBS plus 50 μl serum. After incubating for 30-minutes at room temperature, the beads were washed three time...
example 2
Anti-Collagen V Antibodies are Increased in Asthma Patients
[0185]Plasma was obtained from normal volunteers (non smoking adults, age 18-55), and volunteers with documented chronic asthma. Levels of anti-colV antibodies in the asthma patients and control volunteer subjects were measured using the bead assay as outlined in Example 1.
[0186]As shown in FIG. 2, elevated levels of anti-type V collagen antibodies were found in 8 of 20 asthmatics.
example 3
Intravenous Collagen V Prevents Ovalbumin-Induced Airway Hyper-Responsiveness in Mice
[0187]This Example demonstrates that intravenous administration of collagen V prevents ovalbumin-induced airway hyper-responsiveness in this well-established murine asthma model.
[0188]Balb / c mice were injected via tail vein with 100 μg col (V), alone, or col(V) mixed in complete Freund's adjuvant, (CFA), or PBS, or CFA, alone. Seven days later mice received an IP injection of ovalbumin in alum and this was repeated seven days later. Seven days after the last ova / alum injection, mice in each group were challenged with increasing doses of aerosolized ova followed by measurements of airways resistance (PenH).
[0189]The results showed that col(V), alone, abrogated ova-induced airway hyperresponsiveness (see FIG. 3). Further experiments were conducted which confirm these results. These results are summarized below in Table 2.
TABLE 2Col(V) alone abrogates ova-induced airway hyperresponsivenessPBSCol(V)2-WA...
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