Functional nanostructured chitosan coatings for medical instruments and devices
a technology of chitosan and nanostructure, applied in the field of surface of medical instruments and devices, can solve the problems of lack of uniform coating, limited limited conventional coating techniques in terms of morphology or nanostructure of the resulting chitosan, so as to facilitate the function of the surface being coated, facilitate the application of the article being coated, and high uniform
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example 1
Electrodepositing A Chitosan Coating Containing 5-Florouracil Onto A Stainless Steel Surface
[0023]To demonstrate electrodeposition of chitosan coatings incorporating 5-florouracil (5-FU) onto a stainless steel surface according to the present invention, the chitosan and 5-florouracil containing electrolyte is prepared by dissolving 8 mg of chitosan in 45 ml PC:EG (1:4 v / v). An appropriate amount of H2SO4 is added to adjust pH of the electrolyte to pH 4-4.5. The electrolyte is then subject to ultrasonication for 2 hours for proper dissolution. Supporting electrolyte LiClO4 at 5.5 mM or 55 mM, respectively along with 65 mg 5-florouracil are added to the acidic electrolyte. In this example, the ratio of chitosan to 5-FU is 1:8. Stainless steel surface to be coated with the chitosan and 5-FU and the prepared acidic electrolyte are set up according to FIG. 1 and undergo electrochemical deposition at −5V for 40 mins.
[0024]FIGS. 2A-D show that a 3-dimensional flower-like morphology and an ...
example 2
Electrodepositing A Chitosan Coat With Calcium Carbonate (CaCO3) Onto Titanium Surface
[0026]The same conditions and procedures as illustrated in Example 1 are applied to electrodeposit chitosan coatings with CaCO3 onto a titanium surface. Instead of florouracil, 40 mg of CaCO3 and 55 mM LiClO4 are added to the electrolyte solution. The ratio of chitosan to CaCO3 is 1:5. The morphology of chitosan coating having CaCO3 incorporated therein prepared according to the present method is observed and MC3T3-E1 cell viability on bare titanium versus coated titanium is tested. The results show that not only does the addition of CaCO3 to the chitosan can significantly promote cell adhesion and proliferation as compared to bare titanium surface and the chitosan coating alone (FIG. 4B), the CaCO3 incorporated chitosan coating is also observed to form a nanoflake morphology on the titanium surface under the SEM (FIG. 4A). The results in this Example further demonstrate that the present method of ...
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