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Methods for producing high levels of carboxylic acids by lignocellulosic biomass processing

a technology of lignocellulosic biomass and carboxylic acids, which is applied in the direction of biofuels, microorganisms, biochemical equipment and processes, etc., can solve the problems of complex nutritional requirements, waste, and vast poorly exploited cellulosic biomass resources, and achieve high levels of acids

Inactive Publication Date: 2014-12-11
DIREVO INDAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach reduces the number of processing steps, increases efficiency, and makes the production of carboxylic acids more economically feasible by utilizing a broader range of microorganisms to convert recalcitrant biomass into biofuels and other commercially desirable materials, with the microorganisms showing high yield and low product inhibition, and ability to operate at elevated temperatures.

Problems solved by technology

However, the fastidious lactic acid bacteria have complex nutritional requirements and the use of corn as the feedstock competes directly with the food and feed uses.
Thereofre, the industry of producing fermentation products such as lactic acid is facing the challenge of redirecting the production process from fermentation of relatively easily convertible but expensive starchy materials, to the complex but inexpensive lignocellulosic biomass such as plant biomass.
Cellulosic biomass is a vast poorly exploited resource, and in some cases a waste problem.
Each processing step can make the overall process more costly and, therefore, decrease the economic feasibility of producing biofuel or carbon-based chemicals from cellulosic biological material.

Method used

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  • Methods for producing high levels of carboxylic acids by lignocellulosic biomass processing
  • Methods for producing high levels of carboxylic acids by lignocellulosic biomass processing
  • Methods for producing high levels of carboxylic acids by lignocellulosic biomass processing

Examples

Experimental program
Comparison scheme
Effect test

example 1

Isolation and Cultivation

[0141]All procedures for enrichment and isolation of the strains listed in table 1 employed anaerobic technique for strictly anaerobic bacteria (Hungate 1969). The strains were enriched from environmental samples at temperatures higher than 70° C. with crystalline cellulose and beech wood as substrate. Isolation was performed by picking colonies grown on solid agar medium at 72° C. in Hungate roll tubes (Hungate 1969).

[0142]The cells are cultured under strictly anaerobic conditions applying the following medium:

Basic mediumNH4Cl1.0gNaCl0.5gMgSO4 × 7 H2O0.3gCaCl2 × 2 H2O0.05gNaHCO30.5gK2HPO41.5gKH2PO43.0gYeast extract (bacto, BD)0.5gCellobiose5.0gVitamins (see below)1.0mlTrace elements (see below)0.5mlResazurin1.0mgNa2S × 9 H2O0.75gDistilled water1000.0mlTrace elements stock solutionNiCl2 × 6H2O2gFeSO4 × 7H2O1gNH4Fe(III) citrate, brown, 21.5% Fe10gMnSO4 × H2O5gCoCl2 × 6H2O1gZnSO4 × 7H2O1gCuSO4 × 5H2O0.1gH3BO30.1gNa2MoO4 × 2H2O0.1gNa2SeO3 × 5H2O0.2gNa2WoO4 × 2...

example 2

HPLC

[0146]Sugars and fermentation products were quantified by HPLC-RI using a Via Hitachi LaChrom Elite (Hitachi corp.) fitted with an Rezex ROA Organic Acid H+ (Phenomenex). The analytes were separated isocratically with 2.5 mM H2S04 and at 65° C.

example 3

Phylogenetic Analysis of 16S rDNA Genes

[0147]Genomic DNA was isolated from cultures grown as described above and 16SrDNA amplified by PCR using 27F (AGAGTTTGATCMTGGCTCAG; SEQ ID No. 8) as forward and 1492R (GGTTACCTTGTTACGACTT; SEQ ID No. 9) as reverse primer. The resulting products were sequenced and the sequences analyzed using the Sequencher 4.10.1 software (Gene Codes Corporation). The NCBI database was used for BLAST procedures.

[0148]Sequencing of 16S rDNA from all strains listed in table 1 revealed all these had (at least) one copy of a 16S rDNA operon which was most closely related to Caldicellulosiruptor saccharolyticus (Strain Tp8T =DSM8903) in the available public databases. Alignment was carried out using ClustalW (Chenna et al. 2003) and the phylogenetic tree was constructed using software MEGA4 (Kumar et al. 2001). The tree for all strains listed in table 1 is displayed in FIG. 1.

[0149]The 16S rDNA sequences of all strains listed in table 1 have 99% percent identity to ...

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Abstract

The technology relates, in certain aspects, to the use of novel extreme thermophile microorganisms, which are able to convert lignocellulosic biomass to carboxylic acids, in particular to lactic acid and / or acetic acid, salts or esters thereof.

Description

FIELD OF THE DISCLOSURE[0001]The present disclosure relates to novel methods for converting lignocellulosic biomass material for the production of carboxylic acids like lactic acid.BACKGROUND[0002]Many carboxylic acids are produced industrially on a large scale. They are also pervasive in nature. Carboxylic acids are used in the production of polymers, pharmaceuticals, solvents, and food additives. Industrially important carboxylic acids include acetic acid (component of vinegar, precursor to solvents and coatings), acrylic and methacrylic acids (precursors to polymers, adhesives), adipic acid (polymers), citric acid (beverages), ethylenediaminetetraacetic acid (chelating agent), fatty acids (coatings), maleic acid (polymers), propionic acid (food preservative), terephthalic acid (polymers).[0003]Lactic acid is widely used in food, pharmaceutical and textile industries. It is also used as a source of lactic acid polymers which are being used as biodegradable plastics. The physical p...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12P7/40C12P7/54C12P7/56
CPCC12P7/40C12P2201/00C12P7/54C12P7/56C12N1/20C12P3/00C12P7/065C12N1/205C12R2001/01Y02E50/10C12P7/10C12P7/62
Inventor CURVERS, SIMONSVETLICHNYI, VITALY
Owner DIREVO INDAL BIOTECH