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Large ring lactone compound containing meta-phenol ring

A technology of resorcinol ring and macrolide, applied in the field of macrolide compounds, can solve the problem of long adult period, strong drug resistance, no pine wood nematode and no drug or method for P. research reports, etc.

Inactive Publication Date: 2008-05-21
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is only suitable for protecting precious trees, and the effect is not ideal, and it is highly toxic to humans and animals
[0005] At present, the method of controlling pine wood nematode disease is mainly based on spraying insecticides to control Monochamus alternatus, but due to the long adult period and strong drug resistance of Monochamus alternatus, the control effect is not ideal
So far, no effective and low-toxic special pesticides for killing pine wood nematodes that can be practically applied have been studied, and there are no research reports on agents or methods for both treating pine wood nematodes and Monochamus alternatus

Method used

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  • Large ring lactone compound containing meta-phenol ring
  • Large ring lactone compound containing meta-phenol ring
  • Large ring lactone compound containing meta-phenol ring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the separation and preparation of compound 1-3

[0018] a. Seed culture of the fungus Caryospora carllicarpa ZD2:

[0019] Improved PDA medium: 200g of potatoes, 20g of glucose, 1.0g of yeast extract, 6.0g of glycerin, 17g of agar, 1 piece of medical compound vitamin B and C, 1000mL of water, make a plate, pick strains and insert them into the plate, 25 Cultivate for 15 days;

[0020] b. freeze-dry the mycelium of the above-mentioned cultivated C.carllicarpa ZD2, and then use an optional organic solvent (such as methanol, ethanol, propanol, ethyl acetate, tetrahydrofuran, acetonitrile) to extract 4 times by cold immersion, and The concentrated crude extract is absorbed on a solid absorbent material (e.g. crushed natural materials such as kaolin, clay, talc, quartz, etc.; or crushed synthetic materials such as particulate silica gel, alumina, etc.) and chromatographed on a silica gel column Separation, dry loading, gradient elution with petroleum ether / et...

Embodiment 2

[0024] The test for detecting the anti-nematode activity of the compound of the present invention is carried out by conventional liquid immersion method.

[0025] 1. Test drug:

[0026] Dissolve the test sample in a small amount of organic reagent DMSO, and then add a certain concentration of Tween aqueous solution to prepare sample test solutions with the concentrations shown in Table 3. In this solution, the final concentration of DMSO is <3%, and the final concentration of Tween is <5‰. In addition, the same concentration of DMSO was dissolved in an aqueous solution containing 0.5% (V / V) Tween-20 as a control.

[0027] 2. Cultivation and preparation of pine xylophilus

[0028] The cultivation method of pine wood nematode B.xylophilus: insert Botrytis cinerea into a PDA plate, culture at 25°C until the plate is full, insert a medium with B.xylophilus, and culture at 25°C Until the mycelium disappears, the nematodes cover the plate.

[0029] When in use, pick out the medi...

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Abstract

A kind of 3 meta-phenol loop inlaid macrolide compounds for effectively killing pine nematode is prepared from caryospora carllicarpa ZD2 (CGMCC No.0983) through solid fermenting, extracting and separation. It can be used to prepare nemacide.

Description

Technical field: [0001] The invention relates to a class of macrocyclic lactone compound with m-phenol ring integrated, which belongs to the technical field of biological pesticides. Background technique: [0002] The pine wood nematode disease of pine trees is a devastating disease caused by pine wood nematode (Bursaphelenchus xylophilus (Steiner&Buhrer) Nickle) parasitizing in the pine tree species (Pinus sp.) and feeding on nutrients, which leads to the rapid death of trees. It is an important international disease. The quarantine object is listed as the first of forest diseases and insect pests, and is called "smokeless forest fire". Since the disease was discovered in 1905 and reported in 1934, it has been widely distributed in Japan, the United States, Korea, Canada, Mexico, Greece, Portugal and China. Japan's annual cost for the prevention and control of this disease accounts for 93.6% of the cost of forest pest control, accounting for 20% of the total forestry inves...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D313/00C12P7/40A01N43/22A01P5/00
Inventor 董锦艳张克勤周薇
Owner YUNNAN UNIV