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Bacterium for degrading monocron pesticide residue and produced bacterium formulation

A technology for pesticide residue degradation and monocrotophos, which is applied in the field of high biotechnology, can solve the problems of limited use and narrow degradation spectrum of bacterial strains, and achieve the effects of low production and use costs, good removal effect, and protection of the ecological environment

Inactive Publication Date: 2008-06-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
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Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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Examples

Experimental program
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Embodiment Construction

[0015] Examples of the present invention are described below.

[0016] Activate the original seed of the microbial monocrotophos pesticide residue-degrading bacteria m-1 on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, NH 4 NO 3 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH value 7.2-7.5. After the feeding is completed, 121 ℃ high pressure damp heat sterilization, after cooling to 33 ℃, inoculate 500 liters of seeds with the above cultured shake flask strains according to the inoculation amount of 10% tank, cultivated to the logarithmic growth phase, the stirring spee...

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Abstract

The invention provides a degradation bacillus which can dispel the long-lasting phosphorus pesticide residue and its producing bacillus. The strain is Gram's stain reaction hysteroptosis m-1 in the field of Paracoccus.sp.; the main biology characteristic is G-; the thaliana is the short rod type which likes oxygen and dislikes sport; the scavenger enzyme and the oxidative enzyme is positive; it can not use citrate and nitrate to reduction the positive; the denitrifying reaction generates the gas.

Description

1. Technical field [0001] The invention discloses a monocrotophos pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, degrade chemical pesticide residues by means of microorganisms, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/00C12R1/01
Inventor 李顺鹏贾开志洪青何健李晓慧
Owner NANJING AGRICULTURAL UNIVERSITY
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