Method of cultivating selenium-rich pagodatree ear and method of extracting pagodatree ear selenium polysaccharide

A cultivation method, selenium polysaccharide technology, applied in botany equipment and methods, horticulture, fungi, etc., can solve undiscovered problems, achieve the effects of improving medicinal efficacy, reducing the occurrence of diseases, and strong stress resistance

Inactive Publication Date: 2008-07-02
辽宁东方生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on bacteria-selenium-binding products and selenium-enriched Huaier in the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A cultivation method of selenium-enriched Huaier, the method comprising the steps of:

[0019] 1) Preparation of the selenium-enriched Huaier parent species; the selenium-enriched Huaier species is made from the following ingredients in proportions by weight: 200 parts of potatoes, 20 parts of agar, 20 parts of glucose, 10 parts of soybean powder, 2 parts of magnesium sulfate 1 part of zinc sulfate, 5 parts of potassium dihydrogen phosphate, 5 parts of peptone, 1 part of 6-BA, 2 parts of selenium-enriched fertilizer, 100 parts of pagoda tree leaves or flowers, 1000 parts of water;

[0020] Weigh the ingredients according to the above weight ratio, wash and slice the potatoes, wash the leaves or flowers of the locust tree, and then mix them with other ingredients. Filtrate with gauze, divide the filtrate into test tubes, and make the mother seed medium. The medium is autoclaved at 110-130°C and pressure 0.1-0.3Mpa for 2 hours, aseptically inoculated with Huaeria tissue b...

Embodiment 2

[0028] 1) Preparation of selenium-enriched Huaier female species; selenium-enriched Huaier female species is made from the following ingredients in proportions by weight: 150 parts of potatoes, 30 parts of agar, 30 parts of glucose, 5 parts of soybean powder, 1 part of magnesium sulfate 0.5 parts of zinc sulfate, 10 parts of potassium dihydrogen phosphate, 10 parts of peptone, 0.5 parts of 6-BA, 1 part of selenium-enriched fertilizer, 50 parts of locust tree leaves or flowers, and 500 parts of water;

[0029] 2) Preparation of the original seed of selenium-enriched Huaier; the original seed of selenium-enriched Huaier is made from the following components in proportion by weight: 30 parts of pagoda tree chips, 30 parts of hard miscellaneous wood chips, 10 parts of wheat bran, 10 parts of cornmeal, soybean 10 parts of powder, 1.5 parts of gypsum, 1 part of magnesium sulfate, 1 part of zinc sulfate, 0.15 parts of 6-BA, 1.5 parts of brown sugar, 1.5 parts of white ash, 1 part of p...

Embodiment 3

[0033] The selenium-enriched Huaier mother seed is made from the following ingredients in proportion by weight: 250 parts of potatoes, 10 parts of agar, 10 parts of glucose, 15 parts of soybean powder, 3 parts of magnesium sulfate, 1.5 parts of zinc sulfate, potassium dihydrogen phosphate 1 part, 1 part of peptone, 1.5 parts of 6-BA, 5 parts of selenium-enriched fertilizer, 150 parts of pagoda tree leaves or flowers, 1500 parts of water;

[0034] The original seed of selenium-enriched Huaier is made from the following ingredients in proportion by weight: 40 parts of pagoda tree chips, 40 parts of hard wood chips, 20 parts of wheat bran, 2 parts of cornmeal, 1 part of soybean powder, 0.5 parts of gypsum, magnesium sulfate 0.1 part, 0.1 part of zinc sulfate, 0.05 part of 6-BA, 0.5 part of brown sugar, 0.5 part of white ash, 0.1 part of potassium dihydrogen phosphate, 0.5 part of selenium-enriched fertilizer, 60 parts of water, and the pH value is 7;

[0035] The steps of the cul...

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Abstract

The selenium-rich pagodatree ear mother culture medium consists of selenium-rich fertilizer, potato, agar, glucose, bean powder, magnesium sulfate, zinc sulfate, potassium dihydrogen phosphate, peptone, 6-BA, mulberry leaf or twig, and water. The selenium-rich pagodatree ear original culture medium consists of selenium-rich fertilizer, pagodatree sawdust, hardwood sawdust, wheat bran, corn powder, gypsum, bean powder, magnesium sulfate, zinc sulfate, potassium dihydrogen phosphate, 6-BA, and water, etc. Through preparing selenium-rich pagodatree ear original culture medium and three- stage culture medium, culturing selenium-rich pagodatree ear and further preparation, pagodatree ear selenium polysaccharide is prepared. The present invention has excellent selenium enriching condition, high pagodatree ear resistance, low contamination rate, high output and quality of pagodatree ear selenium polysaccharide, and other advantages.

Description

technical field [0001] The invention relates to a cultivation method and application of medicinal fungi, in particular to the cultivation of selenium-enriched Huaier and the extraction method of Huaier selenium polysaccharide. Background technique [0002] Huaier is an excellent strain of fungi known to the public to have a good anti-cancer effect. Its medicinal value was first recorded in Ge Hong's "The Back of the Elbow". Folks often use its fruiting body as medicine to treat various diseases. Because Huaier's natural resources are seriously insufficient, this creates a huge gap in the huge market demand, and the contradiction between supply and demand is quite prominent. The artificial cultivation period is long and has not yet formed a scale, so it is difficult to provide what the market needs. Selenium also has high medicinal value. It is an indispensable element in the process of human growth and metabolism. It can improve the immune function of organisms, resist viru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C08B37/00C12N1/14
Inventor 崔澎
Owner 辽宁东方生物技术研究所
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