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Method for creating new germ plasm of ransgene tomato containing high hycopene

A high-lycopene, transgenic technology, applied in the field of plant biology, can solve problems such as fruit shrinkage, tomato plant fruit quality traits, abnormal plant growth, etc.

Inactive Publication Date: 2008-10-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the previous studies used constitutive expression promoters, which greatly affected the growth of tomato plants and other quality traits of tomato fruits, such as abnormal plant growth and smaller fruits, etc., which affected its high-quality tomato breeding. Harnessing value [Fraser PD, Proc NatlAcad Sci 2002, 99:1092-1097]

Method used

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  • Method for creating new germ plasm of ransgene tomato containing high hycopene
  • Method for creating new germ plasm of ransgene tomato containing high hycopene
  • Method for creating new germ plasm of ransgene tomato containing high hycopene

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Experimental program
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Embodiment

[0033] 1), material:

[0034] The seeds of the tomato inbred line "T01-254" (Lycopersicon esculentum Mill) were cultivated and preserved by the Institute of Vegetables, Zhejiang University.

[0035] The Taq enzyme used in the PCR reaction was purchased and synthesized by Shanghai Sangon Company. Restriction enzymes and ligases were purchased from Fermentas and Promega; nylon membranes for hybridization were purchased from AmershamPharmacia Biotech, USA; UNIQ-10 Column DNA Gel Recovery Kit and X-Gluc were purchased from Shanghai Sangon Bioengineering Company , the probe labeling kit was purchased from Treasure Bioengineering (Dalian) Co., Ltd., the Trizol reagent was purchased from Life Technologies, and the PCR primers were synthesized by Shanghai Bioengineering Company. The remaining antibiotics and other reagents are domestic or imported analytically pure products.

[0036] 2), isolation of ySAMdc gene

[0037] Saccharomyces cerevisiae was provided by Dr. Jin Weizheng, Sc...

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Abstract

A method for creating a high-lycopene transgenic tomato includes such steps as inoculating the cDNA fragment containing yeast S-adenomethionine decarboxylase gene to the specific promote expressing the mature tomato fruit, configuring specific expression carrier, transforming the agrobacterium, LBA4404 by the carrier, infecting the cotyledon of tomato, differential culture of said cotyledon in the differential culture medium, planting in field, and multi-generation selfing.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a method for creating new germplasm of high lycopene transgenic tomato. Background technique [0002] Lycopene is the most powerful substance to scavenge oxygen free radicals. Its antioxidant effect is 3 times that of β-carotene and 100 times that of vitamin E. It can prevent and treat various cancers, especially those of the entire digestive system. Cancer, including stomach cancer, prostate cancer and pancreatic cancer. On the one hand, the anti-cancer effect of lycopene realizes its physiological activity by protecting the body cells from oxidative damage. At the same time, lycopene can also prevent the division of cancer cells, and achieve the purpose of cancer prevention and anti-cancer in many ways [Hischberg, Current Opinion in Biotechnology 1999, 10: 186-191]. Lycopene is also a compound closely related to the health of the male reproductive system. After it is absorb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C12N15/82A01H4/00
Inventor 卢钢丁淑丽寿森炎曹家树李建勇任彦
Owner ZHEJIANG UNIV