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Method of continuous culture of anaerobic bacterium

A culture method, anaerobic bacteria technology, applied in the direction of bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of decreased productivity, decreased bacterial activity, unstable fermentation, etc., to improve quality, increase purity, and reduce production cost effect

Inactive Publication Date: 2008-11-19
SANWA SHURUI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For this reason, the bacterial cell concentration culture method carried out by the cell recirculation method decreases the bacterial cell activity due to the inhibition of the final product, making the fermentation unstable, resulting in a significant decrease in productivity

Method used

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  • Method of continuous culture of anaerobic bacterium
  • Method of continuous culture of anaerobic bacterium
  • Method of continuous culture of anaerobic bacterium

Examples

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Effect test

Embodiment 1

[0031] Lactococcus lactis (Lactococcus lactis) I0-1 (JCM7638) independently isolated by the inventor was used. Bacteria that were statically cultured on TGC medium (Difco) that were stored at -85°C were used as inoculum, and inoculated in 100 ml of a solution consisting of 3% glucose, 0.5% yeast extract, 0.5% peptone, and 1% NaCl. The culture medium that was dispensed into the Erlenmeyer flask and sterilized by autoclaving at 120° C. for 5 minutes was used as a seed. Composition and system of culture equipment for lactic acid fermentation image 3 as shown. The main fermentation medium is glucose 5%, yeast extract 0.5%, peptone 0.5%, NaCl 1%, and the sterilization conditions are the same as above. The main fermenter is a glass tank with a full capacity of 1 liter, and a stirrer with a magnetic stirring bar is arranged inside, and the stirrer is slowly stirred at a rotation speed of about 400 rpm. The whole tank was immersed in a water bath, and warm water at 37°C was circul...

Embodiment 2

[0043] Lactococcus lactis (Lactococcus lactis) I0-1 (JCM7638) independently isolated by the inventor was used. Bacteria that were statically cultured on TGC medium (Difco) that were stored at -85°C were used as inoculum, and inoculated in 100ml of a solution consisting of 3% glucose, 0.5% yeast extract, 0.5% peptone, and 1% NaCl. The culture medium that was dispensed into the Erlenmeyer flask and autoclaved at 120° C. for 5 minutes, and cultured for 8 hours was used as a seed. The system configuration of the culture device used was the same as that shown in Example 1. The main fermenter is a glass tank with a full capacity of 1 liter, and a stirrer with a magnetic stirrer is installed inside, and the stirrer is slowly stirred at a rotation speed of about 400 rpm. The whole tank is immersed in a water bath, and the temperature of the culture is controlled at 37°C by circulating warm water at 37°C. A composite glass electrode for pH measurement was installed in the tank, and t...

Embodiment 3

[0048] The bacteria used is Zymomonas mobilis NRRLB-14023. Use the bacteria stored at -85°C to inoculate the bacteria obtained by static culture on the YM medium as the inoculum, and inoculate it in a mixture of glucose 100g, yeast extract 10g, KH 2 PO 4 1g, (NH 4 ) 2 SO 4 1g, Mg(SO 4 )·7H 2 O 0.5 g was dissolved in 1 l of deionized water, and 100 ml was dispensed into a Erlenmeyer flask and subjected to high-pressure sterilization at 120°C for 5 minutes in the culture medium, and the culture cultured for about 8 hours was taken as the bacterial species (seed). System configuration and image 3as shown. That is, the main fermenter is a glass tank with a full capacity of 1 liter, and a stirrer with a magnetic stirrer is installed inside, and the stirring is performed slowly at a rotation speed of about 400 rpm. The entire tank is immersed in a water bath, and the temperature of the culture is controlled by circulation of warm water at 37°C. A composite glass electrode ...

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Abstract

In a continuous fermentation system whereby an organic acid or an alcohol is continuously produced with the use of an aerobic bacterium, a substrate is fed based on the cumulative consumption (expressed in mole number) of an alkali added to regulate the pH value of the liquid culture medium at a constant level to thereby regulate the concentration of saccharides remaining in the liquid culture medium at a constant level.

Description

technical field [0001] The invention relates to a continuous culture method of anaerobic bacteria. Background technique [0002] Among anaerobic bacteria, there are many industrially useful bacteria such as acetobutanol bacteria, lactic acid bacteria, and ethanol-producing bacteria, but practically applicable efficient industrial processes using these anaerobic bacteria are not at all clear. For example, acetone-butanol fermentation is the originator of the modern fermentation industry, and it was actively carried out in various countries around the world until the last world war, but this industry is disappearing completely with the rise of petrochemicals. In addition, although lactic acid bacteria are useful bacteria widely used in the fields of dairy product processing or fermented milk preparation, brewing, and pickle production, as a lactic acid fermentation technology in the organic acid industry, it is different from the modern fermentation industry through aerobic ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/00C12P7/02C12P7/06C12P7/40C12P7/56
CPCC12N1/20C12P7/02Y02E50/17C12P7/40C12P7/56C12P7/06Y02E50/10
Inventor 石崎文彬大森俊郎古田吉史梅本泰史
Owner SANWA SHURUI
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