Gordona terrae C-6 and its desulfurization effect

A Gordonella and strain technology, which is applied in the application field of deep desulfurization of auxiliary fuel oil, can solve the problem that Rhodococcus cannot be removed, and achieve the effect of broad industrial application potential.

Inactive Publication Date: 2008-12-24
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defect that Rhodococcus cannot remove the sulfur element in BT sulfur-containing organic compounds, and obtain a bacterial strain that can remove sulfur element in BT sulfur-containing organic compounds from natural screening

Method used

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  • Gordona terrae C-6 and its desulfurization effect

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1 Screening steps of the Gordona terrae C-6 bacterial strain provided by the present invention

[0047] Collect oil-soaked soil samples from around the high-sulfur oil wells in Gudao Oilfield, Shandong, China, and take 5g of samples suspended in 250mL sulfur-free medium WS in a 250mL triangular flask (WS medium composition: 1000mL deionized water contains: 2.56g of K 2 HPO 4 , 2.08 g KH 2 PO 4 , 1.00 g NH 4 Cl, 0.25g MgCl 2 , 0.001g CaCl 2, 0.005g of triammonium citrate, 5.00g of sodium succinate, pH7.2), 10 to 20 glass beads are placed in the Erlenmeyer flask to break up the soil sample suspension, and the medium is pre-autoclaved at 121°C for 25 minute. Put it in an air-bath shaker and mix for 30 minutes. After standing still, take 1 mL of the upper layer liquid and add it to the enrichment medium (the composition is the above-mentioned basal salt medium plus 0.1-0.3% sulfur-free carbon source and 0.01%-0.05% BT). After 2-3 days of culture at 30°C i...

Embodiment 2

[0049] Embodiment 2 The 16S rRNA sequence PCR amplification, sequencing and comparison of Gordona terrae C-6 bacterial strain of the present invention

[0050] 1. Small amount of bacterial total DNA extraction

[0051] ①Pick a freshly activated single colony on the LB plate and put it in 5mL LB medium, shake and culture at 37°C for 12 hours;

[0052] ②Transfer 3mL of bacterial liquid to a 5mL centrifuge tube, centrifuge at 4°C, 12,000 rpm for 5 minutes, and discard the supernatant;

[0053] ③Wash the bacterial sediment once with 0.5mol / L NaCl, and dry the supernatant as much as possible;

[0054] ④Use liquid nitrogen to fully grind the bacterial sediment into powder;

[0055] ⑤Resuspend the ground bacteria in 1mL 50mmol / L Tris (pH 8.0) buffer, then add 0.2mL freshly prepared lysozyme solution (10mg / mL in 0.25mol / L Tris, pH8.0) and 0.8mL 0.25mol / L EDTA solution, mix well and treat at 37°C for 1 hour, then add 200μL 10% SDS solution, mix well and place at 55°C for 5 minutes; ...

Embodiment 3

[0096] Embodiment 3 prepares the cell liquid culture of Gordona terrae C-6 bacterial strain

[0097] Pick a single colony of Gordonia C-6 cultured on a nutrient agar plate and put it in a test tube containing 5 mL of sterilized basic inorganic salt medium WS-yeast powder, and cultivate it on a shaker at 180 rpm for 36-48 hours. Draw 1mL of the bacterial solution in the test tube and add it to a 500mL conical flask containing 100mL of basic inorganic salt medium WS, then add sterilized BT-ethanol stock solution (to make the BT concentration 0.5mmol / L) and the final concentration 1% sterile glucose stock solution. The Erlenmeyer flask was placed in a shaker at 30° C. and cultured at 180 rpm for 48 hours to prepare a liquid culture solution of Gordonia C-6 cells. Bacteria concentration was determined by the dry weight of cells and the optical density (OD) of cell suspension at 660nm 660 ) The linear relationship between ) was obtained by measuring the OD value of the culture. ...

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Abstract

The invention relates to a Gordona terrae C-6 and its desulfurization effect, wherein the name of the bacterium is Gordona terrae C-6, it has been preserved in China General Microbiological Culture Collection Center (CGMCC), the collection number being CGMCC NO.1361. The bacterial strain is obtained through isolating from oil-water polluted soil around high-sulfur oil wells, and can be serve as catalyst for removing sulfur atoms in benzothiophene types sulfur-containing organic compounds, especially suitable for the removal of benzothiophenes organic heterocyclic sulfur in fossil fuels such as coal, oil and related products.

Description

technical field [0001] The invention belongs to the field of actinomycetes desulfurization, and relates to a new strain C-6 of Gordona terrae, and using the strain as a catalyst to remove benzothiophene (Benzothiophene, BT for short) organic sulfur compounds Sulfur element, to achieve the application of auxiliary fuel oil deep desulfurization. Background technique [0002] The 21st century is the century of environmental protection. As the main force of the chemical industry, the energy industry is facing many challenges and opportunities in terms of environmental protection and technology, and thus a new discipline that intersects the fields of biochemical engineering and environmental protection has been born. With the rapid development of the global industrialization process, the demand for energy has increased sharply, but the main energy in the world today still comes from petroleum fuels. Sulfur in petroleum mainly exists in the form of organic sulfides such as mercap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C12P7/22C12S1/02
Inventor 马挺刘如林李京浩李国强李珊珊
Owner NANKAI UNIV
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