Method and apparatus for detecting drug residue of food

A drug and equipment technology, which is applied in the field of detection of drug or toxin residues in animal food, can solve the problems of high inhomogeneity, interference of detection personnel's judgment, interference of detection results, etc., and achieve the effect of improving detection sensitivity and accuracy

Inactive Publication Date: 2007-10-24
浙江迪恩生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] However, the above prior art has the following disadvantages: 1. The colloidal metal-labeled drug or toxin antibody is mixed with unlabeled drug or toxin antibody, which will competitively inhibit the drug or toxin antibody in the detection sample after it is made into a detection device. Toxins, or colloidal metals that are not bound to antibodies, affect the binding speed of drugs or toxins in the sample and colloidal metal-labeled antibodies through steric hindrance, and interfere with the detection results; 2. When mixing antibodies and colloids to make them bind, Due to the high concentration of local antibodies, the colloidal gold particles that are exposed to antibodies first will bind to multiple antibodies, and then the colloidal gold particles that are exposed to antibodies may bind to one antibody molecule or no antibody. The heterogeneity of the drug or toxin antibody is high, which affects the test results; 3. The protein or macromolecular reagents such as BSA or PEG used for blocking will make the sample in the test due to the existence of steric hindrance. Drugs or toxins cannot be well contacted with the labeled antibody, which affects the sensitivity of

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  • Method and apparatus for detecting drug residue of food
  • Method and apparatus for detecting drug residue of food
  • Method and apparatus for detecting drug residue of food

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Embodiment 1, the preparation method of the ractopamine antibody of colloidal gold label

[0056] First prepare colloidal gold by sodium citrate reduction method, that is, add 1ml of 1% trisodium citrate to boiling 0.01% chloroauric acid aqueous solution, stir for 15 minutes while heating, restore the original volume with distilled water after cooling, and obtain the diameter 40nm colloidal gold was used for subsequent experiments. With 0.1mol / L K 2 CO 3 Adjust the pH of the colloidal gold to 9.0, add 1 mg of rabbit anti-ractopamine antibody (purchased from IDS, USA), stir and label for 10 minutes, centrifuge at 4°C and 12,000 rpm for 1 hour, discard the supernatant, and resuspend the colloidal gold with 5ml PBS for use in The colloidal gold-labeled ractopamine antibody (hereinafter abbreviated as RacAb-Au) was separated by Sephacryl-S200 chromatography column (2.6×100 cm) (purchased from GE, USA).

[0057] We used protein purification chromatography system (FPLC) to...

Embodiment 2

[0058] Embodiment 2, the method for preparing colloidal gold-labeled ractopamine antibody using secondary antibody

[0059] First prepare colloidal gold with sodium citrate reduction method, that is, add 1ml of 1% trisodium citrate in 100ml of boiling 0.01% chloroauric acid aqueous solution, stir while heating for 15 minutes, return to the original volume with distilled water after cooling, and 0.1mol / L K 2 CO 3 Adjust the pH of the colloidal gold to 9.0 to obtain colloidal gold with a diameter of 40nm for subsequent experiments.

[0060] Dissolve 1 mg of goat anti-rabbit (or goat anti-mouse) IgG antibody (purchased from IDS, USA) in 50 ml of K with a concentration of 0.01 mol / L and pH 9.0 2 CO 3 . Use a peristaltic pump to pump 40nm colloidal gold into the beaker with magnetic stirring at a speed of 1ml / min; at the same time, use another peristaltic pump to pump the goat anti-rabbit IgG antibody solution into the beaker with the same speed of 0.5ml / min. in the beaker. I...

Embodiment 3

[0064] Embodiment 3, the method for preparing colloidal gold-labeled clenbuterol hydrochloride antibody

[0065] First prepare colloidal gold with sodium citrate reduction method, that is, add 1ml of 1% trisodium citrate in 100ml of boiling 0.01% chloroauric acid aqueous solution, stir while heating for 15 minutes, return to the original volume with distilled water after cooling, and 0.1mol / L K 2 CO 3 Adjust the pH of the colloidal gold to 9.0 to obtain colloidal gold with a diameter of 40nm for subsequent experiments.

[0066] Dissolve 1 mg of goat anti-rabbit (or goat anti-mouse) IgG antibody (purchased from IDS, USA) in 50 ml of K with a concentration of 0.01 mol / L and pH 9.0 2 CO 3 . Use a peristaltic pump to pump 40nm colloidal gold into the beaker with magnetic stirring at a speed of 1ml / min; at the same time, use another peristaltic pump to pump the goat anti-rabbit IgG antibody solution into the beaker with the same speed of 0.5ml / min. in the beaker. In this way,...

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Abstract

The invention discloses an immunity chromatography device for checking left drug or toxin, comprising a solid medium containing the drug or toxin antibody with a colloid metal mark, and a fiber membrane connected with one end of the solid medium, wherein the fiber membrane is fixed with the drug and toxin. The invention also discloses a relative preparation method of the drug or toxin antibody with the colloid metal mark, and a method for using the device to check the left drug or toxin, with high sensitivity.

Description

technical field [0001] The invention mainly relates to a device and a method for detecting drug or toxin residues in animal food, especially a test paper for detecting ractopamine and an application method thereof. The present invention also relates to the above detection equipment and the manufacturing method of the key colloidal metal. Background technique [0002] In the breeding of food animals, illegal use or excessive use of ractopamine, clenbuterol hydrochloride, chloramphenicol and other drugs is common, and some even caused serious damage to people's health; Residues of toxins such as botulinum toxin are also necessary, so efficient and sensitive detection methods are needed to effectively prevent such animal food from entering the consumer market. For example, ractopamine (Ractopamine, abbreviated as Rac herein) is a phenylethanolamine β2-adrenoceptor agonist, which can selectively excite the β2 receptors of smooth muscle, and is mainly used clinically for the tre...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/549G01N33/577G01N33/74
Inventor 朱成钢王旻子张明洲魏建良程烨
Owner 浙江迪恩生物科技股份有限公司
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