Engineering bacterium producing 5-glycyl ethylformic acid and construction and application method thereof

A technology of aminolevulinic acid and engineering bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of long cultivation period, many by-products, cumbersome steps, etc., and achieve low cost and controllability The effect of high reliability and simple process
CN101063105AInactive Publication Date: 2007-10-31ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2007-10-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method to prepare engineering bacteria of 5-glycyl propionic acid and methods for using them, which comprises the following steps: incorporating radial agrobacteriocin; setting the preserved number at CGMCC No. 1938; activating engineering bacteria with LB medium flat; getting monoclonal of the engineering bacteria; seeding to shake; culturing; getting first-grade seed; seeding the first-grade in shake; culturing; getting second-grade seed; seeding the second-grade seed in fermenter; culturing; cooling and evoking with isopropyl-beta-D-sulfo-galactose-glycoside; culturing continually; proceeding supplementing material culture. This engineering bacteria possesses higher ALA synthase expression, simple craft, low cost and good industrial prospect.
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Description

technical field

[0001] The invention relates to an engineering bacterium for producing 5-aminolevulinic acid and its construction and application method. Background technique

[0002] 5-Aminolevulinic acid (5-aminolevulinic acid, ALA) is widely present in organisms, and it is the synthesis of heme, chlorophyll, vitamin B 12 common precursor of tetrapyrrole compounds. As a photodynamic agent (photodynamic agent, PDT), ALA has a wide range of uses in the fields of agrochemicals and medicine. In the field of agriculture, ALA has many functions such as weeding, insecticide, increasing plant stress resistance and promoting plant growth, etc., and is easy to degrade without residue, non-toxic to humans and animals, and has become a very promising pollution-free green agrochemical. In the field of medicine, as a new generation of photodynamic agents, it has significant advantages such as low toxicity and high selectivity. It is used to treat various cancers such as skin cancer, b...

Claims

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