Oral compositions for prevention and reduction of bacterial adhesion to oral surfaces
A composition and oral technology, used in oral care, medical science, dentistry, etc., can solve problems such as difficulty in brushing or wiping the tongue
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Embodiment I
Example 1 - Dentifrice Formulations Containing Anti-Adhesion Enzymes
[0016] Enzymes are formulated in silica matrix formulations. Table 1 provides exemplary dentifrice formulations. All values are percent by weight unless otherwise indicated. The key formulation ingredients in the formulation are ficin, papain and antarctic krill protease enzymes at 0.226% by weight, a mixed silica abrasive system for high quality cleaning at 25% by weight, and A mixed surfactant system of 0.5% SLS / 1.5% pluronic and 1% betaine by weight each. Also included are mint-spearmint flavors stabilized in the presence of enzymes. The various components are individually selected for imparting properties such as foam, aroma, mouthfeel and aesthetics without compromising the activity of the enzyme. For enzyme concentrations, dose-response studies to select effective levels of enzymes were performed prior to selection of clinical formulations. Based on in vitro studies, preparations for clinical u...
Embodiment II
[0017] To test the efficacy of active substances in toothpaste formulations, saliva-coated hydroxyapatite (SHAP) discs were used as artificial teeth, and bacterial cultures consisting of the dominant bacteria in the human oral cavity flowed at a rate consistent with saliva in humans. through the system. Eight discs from each treatment were then placed in a chemostat flow cell and oral bacterial cultures flowed through the discs overnight (20-24 hours) to determine whether the active species coated on the disc surface prevented biofilm form. After 24 hours the SHAP discs were removed and the amount of bacteria was determined. Then measure and analyze the ABS of the solution 610 . Use this result to estimate the amount of bacteria attached to the SHAP disc. From this value, the paste was evaluated for percent anti-adhesive efficacy relative to a matched negative control. Two protease concentrations were tested for each of the three proteases, namely 0.226% by weight and 0.0...
Embodiment III
Example III: In vivo clinical evaluation
[0018] Two human clinical studies were conducted to test the anti-adhesive efficacy of the product relative to a matched negative control paste. The clinical procedure for judging the efficacy of anti-adhesion in terms of the ultimate gain in plaque reduction is indicated below.
a. Modified Gingival Marginal Plaque Index (MGMPI) - Test Product 0.226% Fig Proteases and matched negative controls
[0019]Fifteen (15) in-house subjects were recruited and enrolled in this clinical study. Fourteen (14) completed the 3-week study. One subject was excluded from the study due to mild illness requiring antibiotic treatment. All personnel go to the dental clinic for oral examination and medical history examination. All acceptable subjects received prophylaxis (teeth cleaning) and started a week-long cleaning with Colgate Great Regular. During the treatment phase of the clinical study, each subject reported to the dental office on a de...
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