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Method for extracting refined isorhamnetin in seabuckthorn fruit pulp

A technology of isorhamnetin and sea buckthorn fruit is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., which can solve the problems of low flavonoid content, complicated pretreatment and regeneration process, etc., and achieves low cost. , the effect of simple extraction process

Active Publication Date: 2008-02-20
SHANXI INST OF COAL CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention patent "Method for preparing flavonoids (glycosides) from seabuckthorn leaves by resin adsorption method" (97108475.0) provides a method for purifying seabuckthorn leaf flavonoids by macroporous resin adsorption method. The flavonoid content is not very high
And there is no report about the extraction of isorhamnetin from seabuckthorn fruit puree in the existing literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 〔1〕Take 100g of seabuckthorn puree and put it into the extractor for supercritical CO 2 Extraction, the extraction pressure is 18Mpa, the extraction temperature is 32°C, the separation pressure is 6Mpa, the separation temperature is 25°C, and 84g of defatted fruit puree is obtained;

[0022] [2] Extract the defatted fruit puree with 840ml of water at room temperature twice (1h for the first time, 0.5h for the second time), and filter to obtain 82.5g of filter residue;

[0023] (3) using a pH of 7 and a concentration of 60v% ethanol aqueous solution as the extractant;

[0024] [4] The filter residue after leaching is leached three times at 30°C with 500ml extractant prepared in step [3] (4h for the first time, 2h for the second time, 1h for the third time), filter, combine the filtrates, concentrate and recover ethanol, The obtained solid was washed with water and dried to obtain crude isorhamnetin, which was then dissolved in absolute ethanol, filtered, part of the sol...

Embodiment 2

[0026] 〔1〕Take 100g of seabuckthorn puree and put it into the extractor for supercritical CO 2 Extraction, the extraction pressure is 28Mpa, the extraction temperature is 49°C, the separation pressure is 9Mpa, the separation temperature is 58°C, and 82g of defatted fruit puree is obtained;

[0027] [2] Extract the defatted fruit puree with 840ml of 10v% ethanol aqueous solution at room temperature twice (1h for the first time, 0.5h for the second time), and filter to obtain 80g of filter residue;

[0028] (3) Aqueous ethanol solution with a pH value of 8 and a concentration of 70v% was prepared as an extractant with ammonia water;

[0029] [4] The filter residue after leaching is extracted twice at 35° C. with the 560ml extractant prepared in step [3] (3h for the first time, 1.5h for the second time), and filtered, and the combined filtrate is adjusted to neutrality with hydrochloric acid, Concentrating and recovering ethanol, the obtained solid was washed with water and drie...

Embodiment 3

[0031] 〔1〕Take 100g of seabuckthorn puree and put it into the extractor for supercritical CO 2 Extraction, the extraction pressure is 38Mpa, the extraction temperature is 58°C, the separation pressure is 12Mpa, and the separation temperature is 45°C to obtain 80g of defatted fruit puree;

[0032] [2] Extract the defatted fruit puree with 1200ml of 15% ethanol aqueous solution at room temperature twice (1h for the first time, 0.5h for the second time), and filter to obtain 79g of filter residue;

[0033] (3) sodium hydroxide is used to prepare a pH value of 9 and a concentration of 90v% aqueous methanol as an extractant;

[0034] 〔4〕The filter residue after leaching is leached twice with 790ml extractant prepared in step 〔3〕at 40°C (3h for the first time, 1.5h for the second time), and filtered, and the combined filtrate is adjusted to neutral with hydrochloric acid , concentration and recovery of methanol, the obtained solid was washed with water and dried to obtain crude iso...

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Abstract

A method for extracting refined isorhamnetin from sea buckthorn fruit paste is that: degreased sea buckthorn, which is acquired by extracting the raw material sea buckthorn, is removed of water-soluble impurities through 0-30v percent of alcohol-water solution, and the filtered residue is extracted by 60v percent of alcohol-water solution with pH value of 7-12, anhydrous methanol or anhydrous alcohol, then the extracted liquid is neutralized, steamed and re-crystallized, thereby acquiring the isorhamnetin. The invention has the advantages that: the technology is simple, the cost is low, the product is suitable for industrial production and isorhamnetin content in the product is as high as 80-95wt percent.

Description

technical field [0001] The invention belongs to a method for extracting high-purity isorhamnetin from seabuckthorn fruit puree. technical background [0002] Seabuckthorn fruit puree is a product obtained from seabuckthorn juice through sedimentation and fermentation. It is rich in vitamin C, seabuckthorn flavonoids, organic acids, seabuckthorn polysaccharides, pectin, fruit oil and other ingredients. [0003] Isorhamnetin has important physiological activities, has the functions of protecting liver and reducing enzymes, anti-myocardial ischemia, reducing blood fat, anti-tumor, regulating immune system, etc., can eliminate free radicals in the body, prevent and treat cardiovascular diseases, and directly eliminate free radicals. Its scavenging effect is stronger than that of vitamin E, and its anti-aging effect is obvious. The content of isorhamnetin is the highest among seabuckthorn flavonoids, and the content of isorhamnetin is usually about 0.6wt%. At present, seabuckth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30A61K36/185
Inventor 侯相林刘鹏飞邓天昇王建国
Owner SHANXI INST OF COAL CHEM CHINESE ACAD OF SCI
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