Method for catalyzing and enhancing chemical luminescence by nano particles

A nanoparticle, enhanced chemistry technology, applied in the nano field, can solve the problems of complex operation, unstable metal ions, unstable chemiluminescence system, etc., and achieve the effects of improving sensitivity, increasing luminescence intensity, and simple preparation method.

Inactive Publication Date: 2008-03-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the price of biological enzyme reagents is relatively expensive, the catalytic performance of enzymes is related to environmental factors, making the chemiluminescent system unstable; Fe(II) metal ions are unstable in alkaline systems, so they must be prepared into acidic solutions before adding alkaline solutions , the operation is complex

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Taking silver nanoparticles as an example to catalyze luminol chemiluminescence in hydrazides, the following steps are taken:

[0016] (1) Silver nanoparticles:

[0017] Hydrazine hydrate (N 2 h 4 ·H 2 O) and citric acid (C 6 h 8 o 7 ·H 2 (2) is mixed as a reducing agent, silver nitrate solution is added dropwise in their aqueous solution, centrifuged and washed with water after 30 minutes of rapid stirring reaction, to obtain a silver nanoparticle sol.

[0018] (2) Silver nanoparticles catalyze luminol chemiluminescence

[0019] Dissolve luminol in dilute sodium hydroxide solution (pH 10.5-12) to prepare a 0.1-1.5 mmol / L luminol solution, and add 50 μL of luminol solution and Dilute 50 μL of silver nanoparticles with water to 150 μL / well, add 50 μL of 0.05-1.2 mmol / L hydrogen peroxide solution after mixing, and put it in a chemiluminescence instrument on a microplate to detect the chemiluminescence intensity, which is measured without adding silver nanoparticle...

Embodiment 2

[0021] Taking gold nanoparticles as an example to catalyze the chemiluminescence of Iophine in imidazoles, the following steps are taken:

[0022] (1) Gold nanoparticles:

[0023] Reduction of chloroauric acid (HAuCl) with trisodium citrate 4 ) to obtain gold nanoparticles, and gold nanoparticles with different particle sizes can be prepared by changing the ratio of reactants.

[0024] (2) Gold nanoparticles catalyze the chemiluminescence of profenline

[0025] Dissolve profenline in dilute potassium hydroxide solution (pH 11-12) to prepare a 0.2-2.0 mmol / L profenline solution, and add 50 μL of profenline solution and Dilute 50 μL of gold nanoparticles with water to 150 μL / well, add 50 μL of 0.1 to 1.2 mmol / L hydrogen peroxide solution after mixing, and put it into a chemiluminescence instrument on a microwell plate to detect the chemiluminescence intensity. The chemiluminescence intensity is 6×10 4 . The chemiluminescent intensity after adding gold nanoparticles is 2.3×1...

Embodiment 3

[0027] Taking platinum nanoparticles as an example to catalyze the chemiluminescence of lucigenin in acridines, the following steps are taken:

[0028] (1) Platinum nanoparticles:

[0029] Chloroplatinic acid (H 2 PtCl 6 ) and sodium dihydrogen citrate were stirred at 80°C for 1h, then a small amount of ascorbic acid (C 6 h 8 o 6 ) solution, reacted at 80°C for 30 minutes and then cooled rapidly to terminate the reaction.

[0030] (2) Platinum nanoparticles catalyze lucigenin chemiluminescence

[0031] Dissolve lucigenin in dilute sodium hydroxide solution (pH 11-12) to prepare a 0.05-1.0 mmol / L lucigenin solution, and add 50 μL lucigenin solution and 50 μL platinum Nanoparticles, diluted with water to 150 μL / well, after mixing, add 50 μL of 0.1-1.0 mmol / L hydrogen peroxide solution and put it into a chemiluminescence instrument on a microwell plate to detect the chemiluminescence intensity, and measure the chemiluminescence without adding platinum nanoparticles Strengt...

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Abstract

The present invention belongs to the field of nanometer technology, and is especially method of catalyzing and enhancing the luminous intensity of chemical light-emitting material with nanometer particle. Catalyst of nanometer noble metal particle and oxidant H2O2 are adopted in enhancing the luminous intensity of chemical light-emitting material. The nanometer noble metal particle may be nanometer particle of Au, Ag, Pt, Cu or their mixture or their core-shell structure with core of nanometer oxidant or polymer particle and shell of nanometer noble metal particle. The chemical light-emitting material with greatly enhanced luminous intensity may be applied in inorganic analysis, organic analysis, immunological analysis, DNA hybridization analysis and nucleic acid analysis in high detecting sensitivity.

Description

technical field [0001] The invention relates to a method in the field of nanotechnology, in particular to a method for catalyzing and enhancing chemiluminescence by using nanoparticles. Background technique [0002] About two-thirds of the elements on the earth are metal elements. The unique mechanical, electrical, optical, magnetic, thermal and catalytic properties of metal nanomaterials have attracted increasing attention from researchers. According to reports, metal nanoparticles have been widely used in military, chemical, biomedicine, environment, electromagnetics and other fields. At present, nano-metal cluster catalysts have been regarded as the fourth-generation catalysts in the world. Metal nanomaterials, especially noble metal nanomaterials have the characteristics of electron transfer, large specific surface area, and high surface energy related to particle size and structure. The high catalytic performance of metal nanomaterials has broad application prospects. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/07
Inventor 高峰陈浩崔大祥贺蓉
Owner SHANGHAI JIAO TONG UNIV
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