Method for converting aloeresin a to aloesin
A kind of technology of aloin and its transformation, applied in the direction of organic chemistry, etc., can solve problems such as no commercial value
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Embodiment 1
[0054] Example 1: Acid hydrolysis using HCl and acetone
[0055] Aloe juice containing aloocyanin A (15%) was hydrolyzed over time with 1.3 or 5N HCl at 90° C. in the presence or absence of acetone. Acetone was used to increase the solubility of the substrate in aloe vera juice. Aliquots (20 μl) of the reaction were withdrawn at timed intervals and added to 100 mM phosphate buffer, pH 7.2, containing 30% acetone. Analysis of samples was performed by HPLC using established methods.
[0056] The results showed that the rate of hydrolysis was dependent on the acid strength, with 40% to 80% conversion obtained using 5N HCl. The presence of 10% acetone slightly increased hydrolysis. At higher acid strengths, the rate of aloecyanin A disappearance was greater. The results also showed that in 3 to 5N HCl, 90-95% of aloin A disappeared within 2 h.
Embodiment 2
[0057] Example 2: Using HCl or H 2 SO 4 and acid hydrolysis of ethanol
[0058] Ethanol was used as a substitute for acetone. Use 1N HCl or H in the presence of 50% (v / v) ethanol for the indicated times 2 SO 4 Aloe extract containing aloe cyanin A was hydrolyzed at 90°C. The amount of aloe extract is 10 to 50% (v / v). Aliquots (1 ml) of each reaction were withdrawn at the indicated time intervals and added to 0.5 ml of cold acetone. Analysis of samples was performed by HPLC using established methods.
[0059] Use H 2 SO 4 As hydrolyzed acid and aloe juice concentration of 30%, the mole balance between aloin A and aloin after 4 h of reaction closed in the region of 80% (and possibly higher at this stage). h 2 SO 4 When used as an acid, molar balance closure was about 10% higher compared to HCl, but a faster loss of molar balance was observed.
Embodiment 3
[0060] Example 3: Enzymatic hydrolysis using Aspergillus oryzae protease
[0061] Various conditions were tested in order to preliminarily optimize the enzymatic hydrolysis of aloin A to aloesin in aloe extract or pure aloecyanin A. Aspergillus oryzae protease was chosen as an example of a hydrolytic enzyme and two Aspergillus oryzae proteases were tested, one being Protease M from Amano TM , the other is Bioprotease P conc from Quest International TM .
[0062] Protease M TM illustrate:
[0063] Source: Aspergillus oryzae (Non-GMO)
[0064] Thinner: Potato Dextrin (Non-GMO)
[0065] Thinner content: about 15%
[0066] Bioprotease P conc TM illustrate:
[0067] Minimum 400,000HUT protease u / gm
[0068] -Total viable count<50,000 / gm
[0069] - Yeast & mold <200 / gm
[0070] - Escherichia coli: not present in 25gm
[0071] -Salmonella: Not present in 25gm
[0072] - Conforms to all other international norms
[0073] (heavy metals, lead, arsenic, etc.), as listed b...
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