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Method for converting aloeresin a to aloesin

A kind of technology of aloin and its transformation, applied in the direction of organic chemistry, etc., can solve problems such as no commercial value

Inactive Publication Date: 2008-04-09
CSIR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thus, although aloe cyanin A is present in the greatest amount in aloe juice, it has little or no commercial value

Method used

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  • Method for converting aloeresin a to aloesin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Acid hydrolysis using HCl and acetone

[0055] Aloe juice containing aloocyanin A (15%) was hydrolyzed over time with 1.3 or 5N HCl at 90° C. in the presence or absence of acetone. Acetone was used to increase the solubility of the substrate in aloe vera juice. Aliquots (20 μl) of the reaction were withdrawn at timed intervals and added to 100 mM phosphate buffer, pH 7.2, containing 30% acetone. Analysis of samples was performed by HPLC using established methods.

[0056] The results showed that the rate of hydrolysis was dependent on the acid strength, with 40% to 80% conversion obtained using 5N HCl. The presence of 10% acetone slightly increased hydrolysis. At higher acid strengths, the rate of aloecyanin A disappearance was greater. The results also showed that in 3 to 5N HCl, 90-95% of aloin A disappeared within 2 h.

Embodiment 2

[0057] Example 2: Using HCl or H 2 SO 4 and acid hydrolysis of ethanol

[0058] Ethanol was used as a substitute for acetone. Use 1N HCl or H in the presence of 50% (v / v) ethanol for the indicated times 2 SO 4 Aloe extract containing aloe cyanin A was hydrolyzed at 90°C. The amount of aloe extract is 10 to 50% (v / v). Aliquots (1 ml) of each reaction were withdrawn at the indicated time intervals and added to 0.5 ml of cold acetone. Analysis of samples was performed by HPLC using established methods.

[0059] Use H 2 SO 4 As hydrolyzed acid and aloe juice concentration of 30%, the mole balance between aloin A and aloin after 4 h of reaction closed in the region of 80% (and possibly higher at this stage). h 2 SO 4 When used as an acid, molar balance closure was about 10% higher compared to HCl, but a faster loss of molar balance was observed.

Embodiment 3

[0060] Example 3: Enzymatic hydrolysis using Aspergillus oryzae protease

[0061] Various conditions were tested in order to preliminarily optimize the enzymatic hydrolysis of aloin A to aloesin in aloe extract or pure aloecyanin A. Aspergillus oryzae protease was chosen as an example of a hydrolytic enzyme and two Aspergillus oryzae proteases were tested, one being Protease M from Amano TM , the other is Bioprotease P conc from Quest International TM .

[0062] Protease M TM illustrate:

[0063] Source: Aspergillus oryzae (Non-GMO)

[0064] Thinner: Potato Dextrin (Non-GMO)

[0065] Thinner content: about 15%

[0066] Bioprotease P conc TM illustrate:

[0067] Minimum 400,000HUT protease u / gm

[0068] -Total viable count<50,000 / gm

[0069] - Yeast & mold <200 / gm

[0070] - Escherichia coli: not present in 25gm

[0071] -Salmonella: Not present in 25gm

[0072] - Conforms to all other international norms

[0073] (heavy metals, lead, arsenic, etc.), as listed b...

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Abstract

The invention provides a process for hydrolytically converting aloeresin A to aloesin by the following reaction: The amount of aloesin available for extraction from sap of aloe plants is thereby increased and the extraction and purification of the aloesin is also made easier and less costly. As aloesin is more commercially valuable than aloeresin A, the process also increases the commercial value of the sap or aloe bitters from the aloe plant. The process optionally also includes the step of separating the aloesin from the p-coumaric acid. Typical hydrolysis steps that are used in the process are acid hydrolysis, base hydrolysis and enzymatic hydrolysis. In the case of acid hydrolysis, the acid is any suitable organic or inorganic acid, such as hydrochloric acid, sulfuric acid, nitric acid or phosphoric acid. In the case of enzymatic hydrolysis, the hydrolytic enzyme is typically an esterase, a lipase or a protease.

Description

Background of the invention [0001] The present invention relates to a method for converting aloeresin A into aloesin. [0002] Aloe vera is a succulent plant known to exist in over 300 species, most of which are native to Africa. Products from Aloe Vera have been used in traditional medicine for centuries, particularly in dermatological applications for the treatment of burns, sores and other wounds. Modern therapeutic observations include anti-inflammatory activity, antitumor activity, antacid activity, antidiabetic activity, tyrosinase inhibitory activity, and antioxidant activity. Aloe products are also widely used in the cosmetic and nutraceutical industries, especially with the recent increase in popularity of natural products. [0003] The main African Aloe species from which medicinal, therapeutic, dermatological or cosmetic applications are derived is Aloe ferox, a species confined to southern Africa. Other species of aloe can also be used for similar purposes. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/22
Inventor L·H·斯廷坎普R·K·米特拉S·J·赫吉V·N·费哈恩
Owner CSIR
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