Micro fluid control array protein chip and its usage method

Abstract

Said protein chip comprises a micro flow control channel layer and a chip substrate; at least one of both above is made of soft substance. It features: the micro flow control layer comprises two layers respectively having a first micro flow control channel and a second micro flow control channel; the first micro flow control channel is used for assembling the capturing probes; the second micro flow control channel is used for adding the tested protein sampler and the detection probes; said first micro flow channel layer and the second micro flow control channel layer are located on the chip substrate and forms 2D micro-array pattern on the chip.

Description

technical field

[0001] The invention relates to the field of protein chips, in particular to a microfluidic array protein chip with a two-dimensional structure and an application method thereof. Background technique

[0002] With the development of proteomics and molecular diagnostics, as an urgently needed miniaturized, integrated, high-throughput, fast and efficient protein analysis technology, protein chip has attracted extensive and deep attention since its birth. In proteomics research, it can replace traditional research methods such as two-dimensional electrophoresis and double yeast hybridization, so as to reduce time, reduce costs and improve accuracy, and obtain rich experimental data, making omics research even more powerful and realizing Fast forward. In terms of molecular diagnostics, it only needs a small amount of samples (microliters or even nanoliters) to realize the simultaneous analysis of multiple disease markers, which can be used for early diagnosis of...

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