Compositions and methods for inhibiting white spot syndrome virus (WSSV) infection

A technology for white spot syndrome and viruses, applied in biochemical equipment and methods, chemical instruments and methods, viruses, etc., can solve undetermined problems

Inactive Publication Date: 2008-04-23
AQUA BOUNTY TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, prior art has not identified the region of Vp28 that interacts with the receptor

Method used

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  • Compositions and methods for inhibiting white spot syndrome virus (WSSV) infection
  • Compositions and methods for inhibiting white spot syndrome virus (WSSV) infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Example 1. Expression of viral proteins and protein fragments

[0090] Four major nucleocapsid and envelope proteins from WSSV were evaluated. The primary and secondary structural motifs of each protein were modeled using the MacVector software package. Predictions of secondary structural features based on the amino acid sequence of each protein were tested using multiple predictive algorithms. The results from each prediction method were averaged and this information was used, along with additional predictive information on hydrophilicity, surface probability, flexibility and antigenicity index, to select each protein fraction to be expressed in the fusion system. Portions of viral proteins that may potentially interact with cellular receptors in the viral host may be exposed on the surface of the protein. Alternatively, the interactive parts of each protein may be contained within a single domain. By using the predictive information, likely portions of each viral protein t...

Embodiment 2

Example 2. Inhibition of WSSV infection using Vp28 protein fragments

[0093] Inhibition of WSSV infection using Vp28 fragments was performed as follows. A total of twelve 9 liter plastic aquariums (31 ppt salinity 30°C) were used to house the animals from the time the animals were received to the end of the experiment. The boxes are distributed randomly on two separate rack systems, each with its own water circulation system. In addition to the test group, two sentinel tanks and two positive control tanks were used to monitor possible pathogen escape from the exposure tank and to determine the virulence of the virus, respectively.

[0094] Six experimental feeds were prepared for bioassays. Two viral fusion proteins, one containing the Vp28 fragment and the other containing the Vp35 fragment, were used alone or in combination at two different concentrations to prepare the extruded feed. Immature vannamei (Penaeus vannamei) were fed the experimental diet for 72 hours prior t...

Embodiment 3

Example 3. White spot syndrome virus challenge

[0098] Pacific white shrimp (Peneaus vannamei, average weight 5 g) were grouped and housed in 9 liter flow boxes on an Aquatic Habitats racksystem. 4-8 animals per box, 3 boxes per group. The artificial sea salt was dissolved in Nano pure distilled water to a final salinity of 28ppt and kept at 28°C. Shrimp were housed in 9 boxes and fed one of 3 different diets. The control diet was Zeigler Brothers SI-35 grow-out diet. Both experimental diets were prepared in the laboratory using ground SI-35 as a matrix. The IgY feed had anti-Vp28 IgY added at 0.1%. Vp28 feed was prepared by adding 40 ml / kg of crude broth from CP Kelco runAB04903 (estimated Vp28 fusion concentration 10-40 g / ton final feed). In this experiment, the Vp28 fusion is a recombinant polypeptide prepared by Invitrogen's PurePro Caulobacter Expression System, in which Vp28 fragment 1E (SEQ ID NO: 4) is fused to the surface array protein RsaA from Caulobacter crese...

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Abstract

The present invention relates to a novel composition useful for inhibiting White Spot Syndrome Virus (WSSV) infection of crustacean animals, particularly those of the genera Penaeus sp. More specifically, the novel composition comprises a polypeptide whose amino acid sequence corresponds to at least a portion of Vp28, a surface protein of WSSV, or an antibody that specifically binds the polypeptide. The polynucleotide sequences encoding the Vp28 polypeptides of the present invention are also disclosed. Further disclosed are methods for using the novel compositions to inhibit WSSV infection in crustacean animals.

Description

[0001] related application [0001] This application claims priority to US Provisional Patent Application No. 60 / 501,614 (filed September 9, 2003), the contents of which are incorporated herein by reference in their entirety. Background of the invention [0002] Viral diseases are a major problem in the global shrimp aquaculture industry, causing great economic losses. White Spot Syndrome Virus (WSSV) is one of the most important viral pathogens. Industry losses due to WSSV exceeded $8 billion from 1995-2002. WSSV-infected shrimp become lethargic, show reduced food consumption, loose cuticles, and often exhibit white spots under the exoskeleton. The virus infects most crustaceans but is lethal only to shrimp. [0003] WSSV virions are enveloped nucleocapsids that are rod-shaped, approximately 275 x 120 nm in size, with a tail-like protrusion at one end of the particle (Wongsteerasupaya Dis. Aqat. Org. 21:69-77, 1995) . The double-stranded circular DNA genome is approximat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/04A61K39/00A23K20/195A61K39/12A61K39/42C07K14/01C12N
CPCA61K39/42A61K2039/523C07K2319/00A61K39/12A61K2039/552C07K16/081C12N2710/18022C12N7/00C07K2317/23C07K14/005C12N2710/18034A61P31/20
Inventor K·R·克里姆佩尔
Owner AQUA BOUNTY TECH INC
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