Method for producing anti-oxidation active peptide by glutelin powder biological enzyme method

A technology of antioxidant active peptides and gluten powder, applied in the direction of fermentation, etc., can solve the problems of not entering the stage of industrialization, and achieve the effect of convenient industrial production, high safety, and rich sources of raw materials

Inactive Publication Date: 2008-04-30
NANJING WILD PANT COMPREHENSIVE UTILIZATION INST THE ALL CHINA SUPPLY & MARKETING COOPERATIV +1
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  • Application Information

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Problems solved by technology

However, this type of technology in China has just started, and most of them have not e

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Taking gluten as raw material to produce antioxidant active peptide

[0039]Measure 500mL of water, when heated to 40°C, add 0.5g of sodium hydroxide, melt and stir evenly, then slowly add 50g of gluten powder at a stirring speed of 60-150rpm to prepare 10% (w / w) gluten powder enzymatic hydrolysis substrate. When the pH value of the substrate dropped to 8.5~9.0, the temperature was 55~60° C., and the Alcalase 3.0T alkaline protease and composite protease (weight ratio 1: 1) of adding 1.0% (w / w) gluten powder amount were hydrolyzed, The stirring speed was controlled at 60-80rpm. With the progress of hydrolysis, the pH of the system decreased gradually. The pH value was adjusted with 20% sodium hydroxide solution to keep it between 6.5-8.5, and the reaction time was 5h. The degree of hydrolysis of the hydrolyzate is measured, and when the degree of hydrolysis reaches 15-20%, it is qualified, and the next step is performed after the hydrolysis is completed. ...

Embodiment 1

[0040] To the mensuration of embodiment 1 product performance: every index of test protein hydrolyzate

[0041] Adopt embodiment 1 to produce gluten active peptide powder and test:

[0042] Protein content 97%; degree of hydrolysis 19.52%; total resistance 2.86U / mg, hydroxyl radical scavenging rate (IC50) 2.75mg.

[0043] And compared with other substances' antioxidant properties:

[0044] Tea polyphenols and vitamin C, which have better antioxidant properties, were selected for comparison with gluten enzymatic hydrolyzed peptides. The comparison results are shown in the table below.

[0045] Antioxidant properties of other substances and comparative results

[0046] Preface

[0047] The results show that: in terms of total antioxidant activity, although there is a certain gap with tea polyphenols, the study believes that the antioxidant activity of gluten hydrolyzed peptide is significant. Gluten hydrolyzed peptides are comparable to vitamin C in terms of hydroxy...

Embodiment 2

[0049] The composite enzyme is composed of trypsin and Alcalase 3.0T alkaline protease (weight ratio 1:50), and the rest of the preparation process is the same as in Experimental Example 1.

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PUM

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Abstract

The invention discloses a method for producing oxidation resistant active peptide by adopting a gluten powder biologic enzyme method. The method comprises the steps that the gluten powder is dissolved in alkaline solution to prepare enzymolysis substrate; the substrate enzymolysis is performed by adopting a multiple protease composite enzymolysis method; and enzyme sterilization, separation, concentration, drying, and product collection are performed. The invention adopts the composite enzymolysis method, the produced peptide has higher antioxidant activity, the sources of the raw material adopted by the method of the invention are rich, the cost is low, the process is simple, the condition is moderate, the environment is not polluted, and the method is convenient for the industrialized production.

Description

technical field [0001] The invention relates to a production method of an active peptide, in particular to a production method of an anti-oxidation active protein active peptide by using gluten powder as a raw material and adopting a biological enzymatic method. Background technique [0002] my country is a large wheat producing country with a large area of ​​wheat planting and high yield. Since 1995, the annual wheat output has stabilized at 100 million tons, and there has been a relative surplus of wheat production in production areas, difficulty in selling grain for grain farmers, and overstocking of stocks. Wheat must take the road of deep processing, transforming into commercial starch, and entering the field of industrial production. The production of gluten is the only way for wheat deep processing. It is the necessary link for wheat to enter the industrial field from commercial grain. It is the beginning of the wheat starch industry chain. industry development. At...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 张卫明杜风光史劲松史吉平朱昌玲孙达峰苏锋
Owner NANJING WILD PANT COMPREHENSIVE UTILIZATION INST THE ALL CHINA SUPPLY & MARKETING COOPERATIV
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