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Method for extracting epigallocatechin-3-gallate from tea polyphenol

A technology for epigallocatechin and gallate, which is applied in the field of separating and extracting epigallocatechin gallate, can solve the problems of low yield, large consumption of raw materials, restricting pharmacological research and drug development, and the like, and achieves a yield High and low consumption of raw materials

Inactive Publication Date: 2008-05-21
OCEAN UNIV OF CHINA
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Problems solved by technology

Nevertheless, the medicinal value of tea polyphenols has not been fully developed and utilized, and the key factor is that the tea polyphenols obtained by the traditional extraction process are a mixture and mixed with a small amount of caffeine (such as CN1562995A, CN1351012, CN1421426A), thus limiting the understanding of It conducts pharmacological research and drug development
In 2007, Chan Tak Hang (WO2007041891) invented a method for extracting monomeric catechins from tea leaves by acetylation, but the direct extraction from tea leaves has the disadvantages of large consumption of raw materials, low yield and complex process

Method used

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Embodiment Construction

[0007] The method uses commercially available tea polyphenols as raw materials, the total phenol content is 80-98% (weight percentage, the same below), and the content of epigallocatechin gallate is 20-50%. In this method, pyridine, triethylamine or diisopropylethylamine are used as solvent and acid-binding agent, and acetylation reagents such as acetyl chloride or acetic anhydride are used to acetylate the hydroxyl groups of polyphenols in tea polyphenols to form esters. The temperature is 0°C-30°C. The acetylated product was separated by silica gel column chromatography to obtain peracetylated epigallocatechin gallate, whose chemical name was 5,7-diacetoxy-2-(3,4,5-triacetylphenyl)- 3,4-Dihydro-chromen-3-ol, 3,4,5-triacetylbenzoate. The silica gel has a particle size of 100-300 mesh, and the eluent used is ethyl acetate and petroleum ether, the volume ratio of the two is 1:0.5-3, and the most suitable volume ratio is 1:2. Hydrolyze fully acetylated epigallocatechin gallate...

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Abstract

A method of distilling epigallocatechin-3-gallate from tea polyphenol is characterized in that acetylating reagent is used for acetylating polyphenol substance hydroxyl in the tea polyphenol; then completely acetylated epigallocatechin-3-gallate is obtained by silica gel column chromatographic separation; furthermore, the completely acetylated epigallocatechin-3-gallate is hydrolyzed under the acid condition and in protonic solvent to obtain the epigallocatechin-3-gallate which is dried to obtain the epigallocatechin-3-gallate without solvent. The present invention has the advantages of little raw material consumption and high yield and can be used for the large scale production of epigallocatechin-3-gallate monomers.

Description

technical field [0001] The invention relates to a method for separating and extracting epigallocatechin gallate from tea polyphenols. Background technique [0002] Tea polyphenols are polyphenolic active substances extracted from tea leaves, and the monomer compounds include catechin (C), epicatechin (EC), gallocatechin (GC), catechin gallate ( CG), epigallocatechin (EGC), epicatechin gallate (ECG), gallocatechin gallate (GCG), epigallocatechin gallate (EGCG), and a small amount Caffeine and catechin polymers. The main component is epigallocatechin gallate, and commercially available tea polyphenols generally contain 30-70% of epigallocatechin gallate. [0003] Recent studies have shown that tea polyphenols have many important biological activities such as anti-tumor, anti-oxidation, anti-inflammation, anti-virus, lowering blood fat, and anti-radiation, among which epigallocatechin gallate has the strongest activity. Based on the good biological activity of tea polyphenol...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 万升标江涛孙东魁秦旭龙张晓瑜
Owner OCEAN UNIV OF CHINA
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