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Diesel oil alkane component degradation bacterium and application thereof

A kind of alkane-degrading bacteria and degrading bacteria technology, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., to achieve stable and efficient degradation performance, good adaptability, and high-efficiency alkane degradation ability

Inactive Publication Date: 2010-05-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a bacterial strain that can degrade petroleum substances efficiently and rapidly in view of the problems faced by the above-mentioned existing petroleum pollutant repair technology

Method used

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  • Diesel oil alkane component degradation bacterium and application thereof
  • Diesel oil alkane component degradation bacterium and application thereof
  • Diesel oil alkane component degradation bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Screening and isolation of alkane-degrading bacteria Burkholderia cepacia GS3C and its alkane degradation performance

[0014] Collect the oily sludge mixture from the sewage treatment station of Guangzhou Petrochemical General Plant, take 5g of the soil sample in 100ml sodium pyrophosphate solution, place it in a shaker and shake overnight. With n-hexadecane as the sole carbon source, the shaking solution was inoculated into an inorganic salt medium and cultivated in a 30C, 150rpm shaker. The composition of the inorganic salt medium (MSN) is as follows (g / L): MgSO 4 ·7H 2 O, 0.2; CaCl 2 ·2H 2 O, 0.02; K 2 HPO 4 , 0.5; KH 2 PO 4 , 0.5; NH 4 NO 3 , 0.5; Trace element solution, 10ml / L. The composition of the trace element solution is (mg / L): FeSO 4 ·7H 2 O, 300; MnSO 4 ·H 2 O, 50; CoCl 2 ·6H 2 O, 106; Na 2 MoO 4 ·2H 2 O, 34; ZnSO 4 ·7H 2 O, 40; CuSO 4 ·5H 2 O, 50. Adjust the pH to 7.0.

[0015] After repeated domestication and separation and purification, an alkan...

Embodiment 2

[0018] Example 2: The effect of the addition of yeast extract on the degradation of alkane-degrading bacteria Burkholderia cepacia GS3C of different initial concentrations of carbon sources

[0019] Using n-hexadecane as the carbon source, the alkane-degrading bacterium Burkholderia cepacia GS3C was inoculated into an inorganic salt medium and cultured for 4 days. A control group without and with yeast extract solution (600 mg / L) was set up, and the concentration of the current carbon source was determined. When it is 750, 3750, 7500mg / L, the degradation rate and degradation rate of n-hexadecane by degrading bacteria. It can be seen from Table 1 that the degrading bacteria GS3C can still grow normally at a high substrate concentration of 7500 mg / L. At the same time, as the concentration of n-hexadecane continues to increase, the degradation rate has a trend of decreasing, but due to the degrading bacteria The available carbon and energy sources are sufficient, but the degradation...

Embodiment 3

[0023] Example 3: The effect of heavy metal Cd on the degradation of alkane-degrading bacteria Burkholderia cepacia GS3C

[0024] The alkane-degrading bacteria Burkholderia cepacia GS3C was inoculated into an inorganic salt medium containing n-hexadecane at a concentration of 750 mg / L, and Cd at concentrations of 0.3, 3, and 9 mg / L were added. 2+ At the same time without adding Cd 2+ The sample of is a control, and the degradation rate of n-hexadecane after 4 days is measured. It can be seen from Table 2 that in the experimental concentration range (0-9mg / L) Cd 2+ It has no toxic effect on degrading bacteria, but has a certain promoting effect.

[0025] This example illustrates Cd with higher environmental biological toxicity 2+ Burkholderiacepacia GS3C is not only non-toxic to alkane degrading bacteria, but also has a certain promoting effect, and has the potential to be used in the remediation of petroleum-heavy metal (Cd) composite pollution system.

[0026] The effect of heavy me...

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Abstract

The invention discloses to a strain of degrading bacteria for alkenes components of diesel and usage thereof. The strain of degrading bacteria for alkenes components of diesel of the invention is Burkholderia cepacia which is capable of degrading n-hexadecane of 750mg / L to be of 200mg / L within four days, and cadmium (Cd) with low concentration (<9mg / L) accelerates the degradation to some extent. When the diesel is taken as the unique carbon source, the degrading bacteria can better degrade the alkenes components (C12-C30) of the diesel. Compared with other bacterial strains, the strain of theinvention has the advantages of high effective capability of degrading alkenes, perfect adaptability and tolerant property of Cd, which can be well applied in the field of bioremediation for water body which is polluted by oil pollutants and polluted by the combination of both oil and heavy metals (Cd).

Description

Technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and specifically relates to a bacterium that degrades diesel alkane components and its application in wastewater biological treatment and environmental pollution restoration. Background technique [0002] Petroleum and its products are currently widely used worldwide, and the problems of water and soil pollution caused by it are becoming more and more serious, and people are paying more and more attention. The total oil production in the world today is about 2.2 billion tons per year, of which 1.75 billion tons are produced by land oil fields. Petroleum pollution alone accounts for 8 million tons of the world’s environment each year. In my country, nearly 600,000 tons enter the environment each year, polluting soil, groundwater, rivers and oceans. After these petroleum substances enter the soil environment, a series of physical events will occur. , Chem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/00C02F101/32
Inventor 党志吴仁人
Owner SOUTH CHINA UNIV OF TECH
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