Continuous production method for L-ammonium lactate based on rhizopus

A rhizopus and ammonium lactate technology, applied in the field of continuous production of L-ammonium lactate based on rhizopus, can solve the problems of high industrialization cost and low fermentation acid production rate, and achieve high recovery rate, simple operation and low production cost Effect

Inactive Publication Date: 2008-05-28
SHANGHAI CHLOR ALKALI CHEM
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

However, its fermentation acid production rate is still relatively low, and it requires specific ammonia-resistant L-lactic acid production strains and specific fermentation equipment, and the cost of industrialization is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The bacterial classification adopted is Rhizopus oryzae (Rh.oryzae) 3.819, purchased from China Microorganism Collection Center;

[0042] The spores of Rhizopus oryzae (Rh.oryzae) 3.819 were inserted into the seed medium for aerobic liquid culture, the culture temperature was 35°C, the culture time was 12 hours, and the number of spores inserted into the seed medium was 3*10 6 Individual / L, ventilated by air, the ventilation rate is 15L / min, the components and contents of the seed medium are shown in Table 1:

[0043] Table 1

[0044] ingredients

[0045] The said starch liquefaction liquid is a liquid that uses α-amylase to liquefy starch, and the weight content of starch is 30%, such as using Novozyme Liquozyme Supra (Liquozyme Supra) to prepare according to the required process Then the culture product of above-mentioned 1L is inoculated to it in volume and is 15L, and the air-flow agitation type fermenter that fermentation medium charging capacity is 10L;...

Embodiment 2

[0055] Adopt the same processing step as embodiment 1, wherein:

[0056] The strains adopted are Rhizopus tonkinensis 3.1179 (Rhizopus tonkinensis), which was purchased from China Microorganism Collection Center; when the strains were cultured and fermented, pure oxygen was passed, and the ventilation rate was 4L / min;

[0057] During fermentation culture, the culture temperature is 34°C, the culture time is 40 hours, and ammonia gas is introduced during the culture, and the pH of the culture system is controlled to be 6.5;

[0058] A 23% (W%) ammonium lactate solution was obtained in the electrodialysis stage. The conversion rate of starch in the whole fermentation process reaches 80%, and the yield in the separation stage is 90%. The components and contents of the seed medium are shown in Table 4:

[0059] Table 4

[0060] ingredients

Dosageg / L

glucose

urea

K H 2 PO 4

MgSO 4 ·7H 2 o

ZnSO 4 ·7H 2 o

60

2.00

...

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Abstract

The invention provides a process for continuously producing L-ammonium lactate based on rhizopus sp., comprising the following steps. (1) Seed culture is processed, any kind of spore of rhizopus sp. is introduced to the seed culture medium to proceed aerobic liquid culture, the components of seed culture medium include carbon source, nitrogen source, inorganic salt and water, and the condensation of microcosmic salt in the seed culture medium is 0.8-1.2 grams every liter. (2) The product achieved by the step (1) is introduced to the fermentation culture medium to proceed aerobic liquid fermentation and culture, ammonia water or free ammonia enters in the fermentation process, the pH of the culture system is maintained to be 5 to 8, and then culture medium is fed-batch and the material is discharged, consequently the fermentation product is achieved, and then the target product of ammonium lactate is collected in the fermentation product. The invention has the advantages of simple operation, greatly reduced manufacture cost, easy separation of ammonium lactate form the fermentation liquid, and high recovery ratio.

Description

technical field [0001] The invention relates to a production method of L-ammonium lactate, in particular to a fermentation production method of L-ammonium lactate. Background technique [0002] Lactic acid is an important organic acid that widely exists in living organisms. Two optical isomers, D-lactic acid and L-lactic acid, can be produced by fermentation. Since the human body and many organisms only contain L-lactate dehydrogenase, it can only metabolize and utilize the L-lactic acid in it, while D-lactic acid cannot be degraded by the human body. The World Health Organization therefore advocates the use of L-lactic acid as a food additive and internal medicine to replace the currently commonly used DL-lactic acid. In addition, L-lactic acid, L-lactate and its polymers are also widely used in medicine, agriculture and chemical industry. Especially in recent years, people use poly-L-lactic acid biodegradable plastics, green packaging materials and household and agricult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/845
Inventor 王健邓兴良黄房生
Owner SHANGHAI CHLOR ALKALI CHEM
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