Enzyme composite bactericide with broad-spectrum high-efficient bactericidal action and its preparation method

A technology of preparation and combination of enzymes, applied in the field of food safety and enzyme preparation, replacing antibiotics in feed, to achieve the effect of ensuring food safety, wide bactericidal spectrum and strong bactericidal ability

Inactive Publication Date: 2008-06-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong specificity of hydrolytic enzymes and the differences in the cell wall structure and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment 1 enzyme combination bactericidal preparation

[0020] Based on the enzyme activity content in the preparation, lysozyme 400~1000U / g, β-1,3-glucanase 20000~80000U / g, β-mannanase 5000~10000U / g and neutral protease 80000~ 140000U / g combination. Using trehalose 30%-50%, xanthan gum 20%-40%, carboxymethylcellulose sodium 15%-30% and pullulan 5%-10% coating agent, the combined enzyme is prepared into microcapsules Or powdered enzyme combination bactericidal preparation.

[0021] Its preparation method is:

[0022] 1) Preparation of combined enzyme: according to the enzyme activity content of the enzyme, convert the enzyme activity into the required quality, and weigh the corresponding quality of lysozyme, β-1,3-glucanase, β-mannanase and Neutral protease, mix well.

[0023] 2) Enzyme activity protection: using a coating agent formula of 30% to 50% trehalose, 20% to 40% xanthan gum, 15% to 30% sodium carboxymethylcellulose and 5% to 10% pullu...

Embodiment 2

[0026] After Salmonella was cultivated for 5 hours, add 0.5 mL of lysozyme solution of optimal concentration in No. 1 test tube, and add 0.5 mL of enzyme combination bactericide solution in No. 2 test tube (the solid bactericide has been made into a solution, and the enzyme activity of each enzyme is listed in the claims). Within the range described in 1, the same below), add 0.5 mL of phosphate buffer solution to No. 3 test tube, and do three repetitions for each No. tube. Then place it in a water bath for 10 minutes and then take it out. Dilute No. 1 test tube 1000 times, No. 2 test tube 100 times, and No. 3 test tube 100000 times. Take 1 mL of each in a sterile petri dish, pour about 15 mL of nutrient agar medium into it, turn it over after cooling, and place it in a constant temperature incubator at 37 °C. After 48 hours of incubation, the cells were counted. The results are shown in Table 1.

[0027] Table 1 Salmonella plate count results

[0028] project

...

Embodiment 3

[0031] After culturing Staphylococcus aureus for 5 hours, add 0.5mL of lysozyme solution with the optimal concentration to No. 1 test tube, 0.5mL enzyme combined fungicide solution to No. 2 test tube, and 0.5mL phosphate buffer solution to No. 3 test tube. repeat. Then place it in a water bath for 10 minutes and then take it out. Dilute No. 1 test tube 1000 times, No. 2 test tube 100 times, and No. 3 test tube 100000 times. Take 1 mL of each in a sterile petri dish, pour about 15 mL of nutrient agar medium into it, turn it over after cooling, and place it in a constant temperature incubator at 37 °C. After 36 hours of incubation, the cells were counted. The results are shown in Table 2.

[0032] Table 2 Plate count results of Staphylococcus aureus

[0033] project

[0034] As can be seen from the results in Table 2, the enzyme combination fungicide has the strongest bactericidal effect, and the number of Staphylococcus aureus is reduced by about 100,000 times com...

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Abstract

Disclosed is an enzyme combination and sterilization preparation having the broad-spectrum high efficiency sterilization function and the preparation method, which relates to the food safety and enzyme preparation technique field. The invention provides the enzyme combination and sterilization preparation which is combined by lysozyme, Beta-1, 3- dextranase, Beta-mannase, enzyme and neutral protein enzyme, and adopts the coating agent formulation of fucose, xanthan gum, sodium carboxymethylcellulose and pullulan to coat the combined enzyme. The invention adopts the multi-enzyme combination and coating technology to optimize the combination of the enzyme, meanwhile protecting the enzyme by the coating technology so as to effectively kill gram positive and negative bacteria with wide control spectrum and high efficiency, thus effectively killing pathogenic bacteria such as salmonella, Escherichia coli and golden yellow staphylococcus, and the invention and is a perfect alternative to antibiotics within feedstuff of pig and poultry for the preservation of animal and plant products. The coating protection promotes the resistance of enzyme towards temperature, oxidant and acid, thereby enhancing the sterilization effects under unfavorable circumstances and expanding the field of applications of enzyme fungicide.

Description

technical field [0001] An enzyme combination bactericidal preparation with broad-spectrum and high-efficiency bactericidal action and a preparation method thereof relate to the technical fields of food safety and enzyme preparations. It also relates to the fields of replacing antibiotics in feed and preservation of animal and plant products, specifically a combination of enzymes with broad-spectrum and high-efficiency bactericidal effects. Background technique [0002] Antibiotics are the most widely used antibacterial drugs, not only for clinical use, but also for livestock and poultry feeding and agriculture. In the past 50 years, the long-term use of antibiotics has led to the emergence of a large number of drug-resistant strains, and the resistance of pathogenic bacteria has increased year by year, resulting in a decline in efficacy and an increase in dosage. Therefore, in 1994, the World Health Organization warned the world about the monitoring results of bacterial res...

Claims

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Application Information

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IPC IPC(8): A23K1/17A23L3/3571C12N9/36C12N9/42C12N9/24C12N9/50A23K20/195
Inventor 李永富史锋代卉乐国伟
Owner JIANGNAN UNIV
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