Method for continuously extracting alkaloids, flavone and polysaccharide active components from mulberry leaves
A technology of active ingredients and alkaloids, which is applied in the field of extracting biologically active ingredients, can solve the problems of difficult separation, low extraction rate, unreported structure and category, etc., and achieve the effect of fast separation rate and high content of ingredients
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[0015] example 1
[0016] Add 400 g of the crushed mulberry leaf raw material to 4 L of 30% ethanol aqueous solution, reflux extraction at 95 ° C, repeat the extraction 3 times, each 30 min, the extracts obtained three times are 2.9 L, 3.7 L, 4.1 L respectively, combined. Extract, filter. The filter residue was air-dried for later use, and the ethanol in the obtained filtrate was evaporated to dryness to obtain an extract, which was then dissolved in 2 L of deionized water, left standing for 2 hours, and centrifuged. The supernatant was passed through 200 g of D4020 macroporous resin and adsorbed at 25 °C for 5 h. Then rinse the macroporous resin with 2L of deionized water, and then use 1L of 60% ethanol aqueous solution to elute the macroporous resin, collect the ethanol eluent, and evaporate the eluent to dryness under reduced pressure to obtain 6.3g of extract, in which total flavonoids The mass content of 31%.
[0017] The solution after flowing through the macroporous ...
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[0019] Example 2
[0020] Add 400 g of pulverized mulberry leaf raw materials into 4 L of 70% ethanol aqueous solution, and reflux for 3 times at 95 ° C for 30 min each time, to obtain three extracts of 2.8L, 3.3L, and 3.8L respectively. Combine the extracts and filter, The filter residue was air-dried for later use, and the ethanol in the obtained filtrate was evaporated to dryness to obtain an extract, and then 4 L of distilled water was added to the extract, left standing for 2 hours, and centrifuged. The supernatant was absorbed through 200 g of H-103 macroporous resin at 30 °C. Then first rinse the macroporous resin with 2L deionized water, then use 1L of 60% acetone aqueous solution to elute the macroporous resin, and evaporate the eluate to dryness under reduced pressure to obtain 7.6g of extract, wherein the mass content of total flavonoids is 36% .
[0021] The solution after flowing through the macroporous resin was adsorbed by 60g of 001×7 cation exchange resin at...
Example
[0023] Example 3
[0024] Add 400 g of pulverized mulberry leaf raw materials to 4L of 30% ethanol aqueous solution, and reflux at 95°C for 3 times, 30min each time, to obtain three extracts of 2.8L, 3.5L, and 4.2L, respectively. Combine the extracts and filter, and filter the residue. Dry for use, evaporate the ethanol in the obtained filtrate to dryness to obtain the extract, then add 4 L of distilled water to the extract, let stand for 2 hours, and then centrifuge. The supernatant was passed through 200 g of D101 macroporous resin and adsorbed at 28°C. Then first rinse the macroporous resin with 2L deionized water, then use 1L of 60% acetone aqueous solution to elute the macroporous resin, and evaporate the obtained eluate to dryness under reduced pressure to obtain 6.5g of extract, wherein the mass content of total flavonoids was 37%.
[0025] The solution after flowing through the macroporous resin was adsorbed by 80g of 001×7×7 cation exchange resin at 25°C for 5h. Fir...
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