Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof

A microbial preparation and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low number of effective viable bacteria, inconvenient transportation, preservation of easily contaminated bacteria, etc., and achieve low price, Improve the success rate of breeding and improve the effect of breeding efficiency

Inactive Publication Date: 2008-07-09
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to aim at the low number of effective viable bacteria and large usage in the existing similar bacterial agent technology; the preservation of liquid products is easy to be contaminated with bacteria, and transportation is inconvenient; the cost is too high due to raw materials and production methods High, high product prices and ot

Method used

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  • Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof
  • Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof

Examples

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Embodiment 1

[0043] Embodiment 1: (recipe and preparation method 1 of product of the present invention)

[0044] A microbial preparation for purifying aquaculture water is prepared according to the following method steps:

[0045] (1) Preparation of medium at all levels, wherein:

[0046] a) PDA slant solid medium: 200g of potatoes, 2g of glucose, 2g of agar, 1 liter of water, set aside;

[0047] b) shake flask liquid culture medium: 200g of potatoes, 2g of glucose, 1 liter of water, set aside;

[0048] c) Seed tank fermentation medium: 12 grams of starch, 7 grams of yeast extract, 28 grams of peptone, 8 grams of beef extract, 945 grams of water, set aside;

[0049] d) Preparation of solid medium: bran: 800 grams, rice bran: 85 grams, glucose 50 grams, bentonite 49.5 grams, K 2 HPO 4 15 grams, D biotin 0.5 grams, water 1000 grams, spare;

[0050] (2) Cultivation of slant strains: inoculate the preservation number: CGMCC No.2288 Bacillus subtilis BJ strain on the PDA slant solid mediu...

Embodiment 2

[0054] Embodiment 2: (recipe and preparation method 2 of product of the present invention)

[0055] The preparation of the seed tank fermentation medium in this embodiment: 20 grams of starch, 10 grams of yeast extract, 15 grams of peptone, 15 grams of beef extract, and 940 grams of water, set aside;

[0056] Preparation of solid medium: bran: 820 grams, rice bran: 130 grams, glucose 25 grams, bentonite 14.9 grams, K 2 HPO 4 10 grams, D biotin 0.1 grams, water 1000 grams, spare;

[0057] All the other preparation methods are the same as in Example 1.

Embodiment 3

[0058] Embodiment 3: (recipe and preparation method 3 of product of the present invention)

[0059] The preparation of the seed tank fermentation medium in this embodiment: 28 grams of starch, 5 grams of yeast extract, 11 grams of peptone, 5 grams of beef extract, and 951 grams of water, set aside;

[0060] Preparation of solid medium: bran: 900 grams, rice bran: 30 grams, glucose 20 grams, bentonite 19 grams, K 2 HPO 4 30 grams, 1 gram of D biotin, 1000 grams of water, set aside;

[0061] All the other preparation methods are the same as in Example 1.

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Abstract

The invention discloses a microbe agent for purifying aquaculture water mass and a method for preparation, which belongs to the aquaculture water mass technique field. The agent comprises the following parts by weight: bran 80-90%, chaff 3-15%, glucose 2-5%, alta-mud 1-5%, K2HPO4 1-3% and VD 0.01-0.10%, wherein equal quantity of water is charged to dispense the solid culture medium, the preservation number is CGMCC No.2288, the germ is Bacillus subtilis BJ which is prepared by classes of fermentation. The agent is cheap in cost, high in bacterial content and the quality assured period is over one year and a half, the pH value of culture period water mass is between 8.02 and 8.60 stably after using, ammonia nitrogen can be reduced to safe level after 5-7 d, nitrite can be maintained in the safe density, the culture success rate can be improved greatly and can be spreaded and used in the aquaculture places.

Description

technical field [0001] The invention relates to the technical field of aquaculture water, in particular to a microbial preparation for purifying aquaculture water and a preparation method thereof. Background technique [0002] "Fish farming first raises water", water quality is the key to the success or failure of aquaculture. However, with the development of intensification and large-scale production of aquaculture, the aquaculture industry is facing a series of serious problems: deterioration of water quality, frequent outbreaks of diseases, proliferation of harmful algae, abuse of chemicals, and decline in the safety of aquatic products. Among them, the deterioration of water quality is the most fundamental of all problems. It is precisely because of the deterioration of water quality that frequent outbreaks of aquatic animal diseases have been aggravated, and the abuse of chemical drugs such as antibiotics has been exacerbated, resulting in a decline in the safety of aq...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125
Inventor 汤江武姚晓红吴逸飞王新李浙烽
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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