Microorganism preparation for purifying aquatic product cultivating water body and preparation method thereof
A microbial preparation and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low number of effective viable bacteria, inconvenient transportation, preservation of easily contaminated bacteria, etc., and achieve low price, Improve the success rate of breeding and improve the effect of breeding efficiency
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Embodiment 1
[0043] Embodiment 1: (recipe and preparation method 1 of product of the present invention)
[0044] A microbial preparation for purifying aquaculture water is prepared according to the following method steps:
[0045] (1) Preparation of medium at all levels, wherein:
[0046] a) PDA slant solid medium: 200g of potatoes, 2g of glucose, 2g of agar, 1 liter of water, set aside;
[0047] b) shake flask liquid culture medium: 200g of potatoes, 2g of glucose, 1 liter of water, set aside;
[0048] c) Seed tank fermentation medium: 12 grams of starch, 7 grams of yeast extract, 28 grams of peptone, 8 grams of beef extract, 945 grams of water, set aside;
[0049] d) Preparation of solid medium: bran: 800 grams, rice bran: 85 grams, glucose 50 grams, bentonite 49.5 grams, K 2 HPO 4 15 grams, D biotin 0.5 grams, water 1000 grams, spare;
[0050] (2) Cultivation of slant strains: inoculate the preservation number: CGMCC No.2288 Bacillus subtilis BJ strain on the PDA slant solid mediu...
Embodiment 2
[0054] Embodiment 2: (recipe and preparation method 2 of product of the present invention)
[0055] The preparation of the seed tank fermentation medium in this embodiment: 20 grams of starch, 10 grams of yeast extract, 15 grams of peptone, 15 grams of beef extract, and 940 grams of water, set aside;
[0056] Preparation of solid medium: bran: 820 grams, rice bran: 130 grams, glucose 25 grams, bentonite 14.9 grams, K 2 HPO 4 10 grams, D biotin 0.1 grams, water 1000 grams, spare;
[0057] All the other preparation methods are the same as in Example 1.
Embodiment 3
[0058] Embodiment 3: (recipe and preparation method 3 of product of the present invention)
[0059] The preparation of the seed tank fermentation medium in this embodiment: 28 grams of starch, 5 grams of yeast extract, 11 grams of peptone, 5 grams of beef extract, and 951 grams of water, set aside;
[0060] Preparation of solid medium: bran: 900 grams, rice bran: 30 grams, glucose 20 grams, bentonite 19 grams, K 2 HPO 4 30 grams, 1 gram of D biotin, 1000 grams of water, set aside;
[0061] All the other preparation methods are the same as in Example 1.
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