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Multi-modification derivant of glutin and crosslinked material thereof

A technology of derivatives and gelatin, applied in the field of multiple modified derivatives of gelatin

Active Publication Date: 2008-07-16
BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, these methods still have many limitations in the preparation of gelatin crosslinked materials

Method used

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  • Multi-modification derivant of glutin and crosslinked material thereof
  • Multi-modification derivant of glutin and crosslinked material thereof
  • Multi-modification derivant of glutin and crosslinked material thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Multiple modified derivatives of acetylated and thiolated gelatin (multiple modified derivatives of gelatin represented by general formula (V) of the present invention, wherein R 1 =-CH 2 CH 2 -, R 2 =-CH 3 ) Synthesis and characterization

[0066] (1) Acetylation modification of gelatin

[0067] One gram of gelatin (Type B, from Cowhide, Sigma, USA) was dissolved in 100 ml of distilled water (about 30 degrees Celsius) to obtain a clear and transparent solution. Use 1.0 mol / L sodium hydroxide solution to adjust the pH of the solution to about 9.5, then add 0.05 g of acetic anhydride (analytical purity) under electromagnetic stirring, and continue to add an appropriate amount of 1.0 mol / L sodium hydroxide solution to keep the solution The pH value is weakly alkaline (usually 8.0 ~ 9.5). The reaction was stirred at about 30 degrees Celsius for 1 hour. After that, the above solution was put into a dialysis tube (with a molecular weight cut off of 3500, Fisher, USA), ...

Embodiment 2

[0077] Example 2 Multiple modified derivatives of hexanoylated and thiolated gelatin (multiple modified derivatives of gelatin represented by general formula (V) of the present invention, wherein R 1 =-CH 2 CH 2 -, R 2 =-CH 2 CH 2 CH 2 CH 2 CH 3 ) Synthesis and characterization

[0078] (1) Caproacylation modification of gelatin

[0079] One gram of gelatin (type A, from pigskin, Sigma, USA) was dissolved in 100 ml of distilled water (about 30 degrees Celsius) to obtain a clear and transparent solution. Use 1.0 mol / L sodium hydroxide solution to adjust the pH of the solution to about 9.5, then add 0.1 g of valeric anhydride (analytical purity) under electromagnetic stirring, and continue to add an appropriate amount of 1.0 mol / L sodium hydroxide solution to keep the solution The pH value is weakly alkaline (usually 8.0 ~ 9.5). The reaction was stirred at about 30 degrees Celsius for 1 hour. After that, the above solution was put into a dialysis tube (with a molecular weight cut of...

Embodiment 3

[0090] Example 3 Multiple modified derivatives of butylated and thiolated gelatin (multiple modified derivatives of gelatin represented by general formula (V) of the present invention, wherein R 2 =-CH 2 CH 2 CH 3 ) Synthesis and characterization

[0091] (1) Butyryl modification of gelatin

[0092] One gram of gelatin (Type B, from Cowhide, Sigma, USA) was dissolved in 100 ml of distilled water (about 30 degrees Celsius) to obtain a clear and transparent solution. Adjust the pH of the solution to about 9.5 with 1.0 mol / L sodium hydroxide solution, then add 0.08 g of butyric anhydride (analytical pure) under electromagnetic stirring, and constantly add an appropriate amount of 1.0 mol / L sodium hydroxide solution to keep the solution The pH value is weakly alkaline (usually 8.0 ~ 9.5). The reaction was stirred at about 30 degrees Celsius for 1 hour. After that, the above solution was put into a dialysis tube (with a molecular weight cut off of 3500, Fisher, USA), dialyzed with dis...

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Abstract

The invention discloses a Gelatin derivative with multiple modifications, which not only has the general formula (I) structure, but also has at least one structure of the general formulas (II), (III) and (IV) at the same time, wherein, G is a Gelatin residue comprising an A-type Gelatin residue, a B-type Gelatin residue and a gene recombination Gelatin residue; R1 is an alkylidene or a polymer chain containing an aminoacyl bond; R2 is an alkyl or an arylamidase; R3 is an alkylidene, while R1 is a carboxyl or a carboxyl salt. The multiple modifications of the Gelatin comprises the aminoacyl bond hydrophobic modification of the Gelatin side chain amino, the aminoacyl bond Carboxylation of the Gelatin side chain amino, the aminoacyl bond sulfhydrylation of the Gelatin side chain amino and the sulfhydrylation after the aminoacyl bond Carboxylation of the Gelatin side chain amino. Besides, the invention also discloses the cross-linked copolymer, which can be used as the cell growth matrix etc. The Gelatin derivative with multiple modifications of the invention has flexible chemical structures and a plurality of performance.

Description

Technical field [0001] The present invention relates to compound gelatin, in particular to multiple modified derivatives of gelatin; in addition, the present invention also relates to crosslinking materials of the multiple modified derivatives of gelatin. Background technique [0002] Gelatin is a denatured derivative of collagen, a protein containing 18 kinds of amino acids that are indispensable to the human body. Natural gelatin is usually prepared by hydrolyzing collagen in connective tissues such as animal skins, bones, tendons, and ligaments by acid or alkali methods, and then separated and extracted. Usually it can be divided into two types: type A and type B: gelatin prepared by acid hydrolysis is called type A, which has fewer side chain carboxylic acid residues and a higher isoelectric point; prepared by alkaline hydrolysis Gelatin is called type B. Most of the glutamine and asparagine residues in the side chain are converted to glutamic acid and aspartic acid, so the s...

Claims

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Application Information

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IPC IPC(8): C07K14/435
CPCA01N1/0231A61K47/42C08H1/06A61K9/06A61L27/222
Inventor 宋婵
Owner BIOREGEN BIOMEDICAL (CHANGZHOU) CO LTD
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