Method for preparing tilapia fishskin small molecular peptide chelate zinc salt

A small molecule peptide, chelated zinc technology, applied in the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problems of side effects, difficult to absorb, etc., and achieve the effect of high safety, low cost, scientific and reasonable process

Inactive Publication Date: 2008-07-23
湖南笑呵呵食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method of tilapia fish skin small molecule peptide chela

Method used

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Examples

Experimental program
Comparison scheme
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Example Embodiment

[0039] Implementation case:

[0040] Weigh 100.0008g of clean tilapia skins, immerse them in 700ml of 0.1mol / L hydrochloric acid solution and 0.1mol / L sodium hydroxide solution for 1hr after degreasing treatment, wash with distilled water to neutrality, and keep at 45℃. Homogenize with 1000ml of distilled water at constant temperature for 12 hours, extract the supernatant by suction filtration, heat up to 50°C-55°C, adjust the pH to 6.0, add 0.04671g papain for constant temperature enzymatic hydrolysis for 2 hours, and then in a water bath at 100°C. After heating for 20 minutes, the enzymatic hydrolysis reaction is terminated, and the fish skin proteolysis solution is obtained. The hydrolysis degree of tilapia fish skin protein is 12.3%.

[0041] A compound decolorant (mixed with 3g / L of activated carbon yeast and cyclodextrin) was used to debitter, decolor and deodorize the fish skin proteolysis solution, and collect the supernatant by centrifugal filtration at 4000 r / min. Pass 0...

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Abstract

The invention discloses a preparation method for tilapia skin small peptide chelating zinc salt, using the tilapia skin as materials, preparing the tilapia skin small peptide (TSP-II) by means of a combination of the enzyme method and the ultrafiltration method. The tilapia skin small peptide chelating zinc salt is prepared with tilapia skin small peptide liquor and zinc sulfate by a water bath heating method. The preparation method for the tilapia skin small peptide chelating zinc salt has the advantages of scientific and rational technique, easy operation, low cost, and providing a zinc supplement preparation easy to be absorbed.

Description

technical field [0001] The invention relates to a preparation method of tilapia fish skin small molecular peptide chelated zinc salt. Background technique [0002] Zinc is an essential trace element for animals, and it is called a "life element" because of its extensive physiological and biochemical functions in the body. When it is lacking, it will lead to a series of adverse reactions and diseases. The main manifestations are: (1) skin lesions; (2) anorexia (with changes in taste and smell sensitivity); (3) growth arrest; (4) poor wound healing or inhibition of protein metabolism; (5) sexual dysfunction ; (6) immune function changes; (7) night vision impairment, vitamin A metabolism changes; (8) diarrhea; (9) depression; (10) deformity. At present, zinc deficiency is an urgent public health problem to be solved in my country and many developing countries. Most children, pregnant women and patients in my country suffer from nutritional zinc deficiency to varying degrees. ...

Claims

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Application Information

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IPC IPC(8): C07K1/34C12P21/06
Inventor 刘成梅梁汉萦刘伟涂宗财梁瑞红
Owner 湖南笑呵呵食品有限公司
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