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Method for preparing tilapia fishskin small molecular peptide chelate zinc salt

A small molecule peptide, chelated zinc technology, applied in the preparation method of peptide, chemical instrument and method, peptide and other directions, can solve the problems of side effects, difficult to absorb, etc., and achieve the effect of high safety, low cost, scientific and reasonable process

Inactive Publication Date: 2008-07-23
湖南笑呵呵食品有限公司
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method of tilapia fish skin small molecule peptide chelated zinc salt, to overcome the disadvantages of side effects and difficult absorption of traditional zinc supplement preparations

Method used

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Examples

Experimental program
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Effect test

Embodiment example

[0040] Weigh 100.0008g of cleaned tilapia skin, after degreasing treatment, soak in 700ml of 0.1mol / L hydrochloric acid solution and 0.1mol / L sodium hydroxide solution for 1hr, wash with distilled water until neutral, and put it under the condition of 45℃ Homogenize with 1000ml of distilled water at constant temperature for 12 hours, extract the supernatant by suction filtration, raise the temperature to 50°C-55°C, adjust the pH value to 6.0, add 0.04671g of papain for enzymolysis at constant temperature for 2hr, and then place it in a water bath at a high temperature of 100°C Heated for 20 minutes to terminate the enzymatic hydrolysis reaction to obtain the enzymatic hydrolyzate of fish skin protein, and the degree of hydrolysis of tilapia fish skin protein was 12.3%.

[0041] The fish skin protein enzymatic hydrolyzate was debittered, decolorized and deodorized with a compound decolorizing agent (3g / L activated carbon yeast and cyclodextrin, etc.), and the supernatant was col...

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Abstract

The invention discloses a preparation method for tilapia skin small peptide chelating zinc salt, using the tilapia skin as materials, preparing the tilapia skin small peptide (TSP-II) by means of a combination of the enzyme method and the ultrafiltration method. The tilapia skin small peptide chelating zinc salt is prepared with tilapia skin small peptide liquor and zinc sulfate by a water bath heating method. The preparation method for the tilapia skin small peptide chelating zinc salt has the advantages of scientific and rational technique, easy operation, low cost, and providing a zinc supplement preparation easy to be absorbed.

Description

technical field [0001] The invention relates to a preparation method of tilapia fish skin small molecular peptide chelated zinc salt. Background technique [0002] Zinc is an essential trace element for animals, and it is called a "life element" because of its extensive physiological and biochemical functions in the body. When it is lacking, it will lead to a series of adverse reactions and diseases. The main manifestations are: (1) skin lesions; (2) anorexia (with changes in taste and smell sensitivity); (3) growth arrest; (4) poor wound healing or inhibition of protein metabolism; (5) sexual dysfunction ; (6) immune function changes; (7) night vision impairment, vitamin A metabolism changes; (8) diarrhea; (9) depression; (10) deformity. At present, zinc deficiency is an urgent public health problem to be solved in my country and many developing countries. Most children, pregnant women and patients in my country suffer from nutritional zinc deficiency to varying degrees. ...

Claims

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Application Information

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IPC IPC(8): C07K1/34C12P21/06
Inventor 刘成梅梁汉萦刘伟涂宗财梁瑞红
Owner 湖南笑呵呵食品有限公司
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