Method for preparing jatropha curcas seed fat by microbiological fermentation process

A microbial fermentation method, a technology for Jatropha curcas seeds, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor comprehensive utilization, low preparation efficiency, and low extraction rate of Jatropha curcas seeds , to achieve the effect of fast breeding of bacteria, improving utilization rate and improving feed protein

Inactive Publication Date: 2008-07-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above methods have their own advantages and disadvantages, but there are problems such as low preparation efficiency, high energy consumption, low extraction rate and poor synthesis and utilization of Jatropha curcas seeds.

Method used

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  • Method for preparing jatropha curcas seed fat by microbiological fermentation process
  • Method for preparing jatropha curcas seed fat by microbiological fermentation process
  • Method for preparing jatropha curcas seed fat by microbiological fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: as figure 1 shown

[0028] Step 1, to the pretreatment of Jatropha curcas seed, see figure 2 ,

[0029] Shell the 100kg jatropha seeds that remove impurities such as moldy particles and soil by screening; use a pulverizer or grind and pulverize, and the particles of the jatropha seeds after the pulverization are 30 to 50 meshes to obtain jatropha seed oil slurry , and then add hot water to the jatropha seed oil slurry to adjust, so that the water content in the jatropha seed reaches 40%, and heat to 100° C. for sterilization.

[0030] Step 2, the fermentation process to Jatropha seed oil slurry

[0031] see image 3 , add 19.8kg of sterilized water containing 2% sugar to 0.2kg of Angel Yeast, and mix it at a temperature of 30°C, place it for 20 minutes to obtain activated microbial bacteria, and mix the activated microbial bacteria into the step 1. Add water to the obtained product to make the moisture content reach 50%, stir evenly, stir and ferment...

Embodiment 2

[0034] Embodiment 2: as figure 1 shown

[0035] Step 1, to the pretreatment of Jatropha curcas seed, see figure 2 ,

[0036] Shell the 100kg jatropha seeds that remove impurities such as moldy particles and soil by screening; use a pulverizer or grind and pulverize, and the particles of the jatropha seeds after the pulverization are 30 to 50 meshes to obtain jatropha seed oil slurry , and then add hot water to the jatropha seed oil slurry to adjust, so that the water content in the jatropha seed reaches 70%, and heat to 100° C. for sterilization.

[0037] Step 2, the fermentation process to Jatropha seed oil slurry

[0038] see image 3 , add 24.5kg of Gao's culture medium to 0.5kg of Geotrichum candidum (also can use Aspergillus oryzae, Rhizopus chinensis or mold MJ18), and mix at a temperature of 35°C, place in a 28-30°C incubator to activate 24- After 48 hours to obtain microbial bacteria, mix the activated microbial bacteria into the product obtained in step 1, and a...

Embodiment 3

[0041] Embodiment 3: as figure 1 shown

[0042] Step 1, to the pretreatment of Jatropha curcas seed, see figure 2 ,

[0043] Shell the 100kg jatropha seeds that remove impurities such as moldy particles and soil by screening; use a pulverizer or grind and pulverize, and the particles of the jatropha seeds after the pulverization are 30 to 50 meshes to obtain jatropha seed oil slurry , and then add hot water to the jatropha seed oil slurry to adjust, so that the water content in the jatropha seed reaches 55%, and heat to 100° C. for sterilization.

[0044] Step 2, the fermentation process to Jatropha seed oil slurry

[0045] see image 3, Add 22kg of Gao's culture medium to 0.3kg of Geotrichum candidum, Aspergillus oryzae, Rhizopus chinensis and mold MJ18, and mix them at a temperature of 33°C, place in a 28-30°C incubator to activate for 24-48 hours After obtaining the microbial bacteria, mix the activated microbial bacteria into the product obtained in step 1, and add w...

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Abstract

The invention discloses a preparation method for Jatropha Curcas seed oil by means of the microbiological fermentation method, which adopts the Jatropha Curcas seeds as the raw materials. The preparation method comprises the following steps: first, shelling, crushing and sterilizing the Jatropha Curcas seeds in order to get Jatropha Curcas seeds oil slurry; second, activating microbial strain, adding 20 - 25 portions of activated microbial strains (pbw) into 100 portions of Jatropha Curcas seeds oil slurry for fermentation, so as to obtain the fermented and grown Jatropha Curcas seeds oil slurry grains; third, adding dissolving agent into the Jatropha Curcas seeds oil slurry grains, after clarification and separation, acquiring the oil cake and A solution; finally, separating the A solution by use of the low pressure vacuum rotating evaporation method, so as to obtain the dissolving agent and the desired Jatropha Curcas seed oil. The preparation method for Jatropha Curcas seed oil by means of the microbiological fermentation method has the advantages of lowering the production costs, improving the extraction rate and efficiency of the Jatropha Curcas seed oil, enabling the comprehensive utilization of the raw materials, and increasing the economic gains of the Jatropha Curcas industry.

Description

Technical field: [0001] The invention relates to a method for preparing jatropha seed oil, in particular to a method for preparing jatropha seed oil with a microbial fermentation method. Background technique: [0002] Jatropha curcas, also known as Jatropha curcas, stinky tung tree, tung oil tree, fake peanut tree, etc., belongs to the genus Jatropha in the family Euphorbiaceae. The oil content of Jatropha curcas seeds is between 40% and 60%. According to expert research, the average yield of wild jatropha seeds is about 660kg / mu, and 180-270kg of biodiesel can be extracted and processed. The biodiesel is suitable for various diesel engines, and its ignition point, freezing point, sulfur content, carbon monoxide And other key indicators are all better than domestic 0# diesel oil, reaching the current European No. 2 diesel standard. [0003] At present, the extraction methods of jatropha seed oil are: [0004] (1), microwave-assisted extraction method: the optimum extracti...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/85C12R1/69C12R1/645
Inventor 邱树毅黄永光王广莉吴远根周剑丽王啸
Owner GUIZHOU UNIV
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