Clone of gene RID1 for controlling rice floral conversion and heading stage and application thereof

A heading date and genetic technology, applied in the field of plant genetic engineering, can solve problems such as non-existence and non-discovery

Inactive Publication Date: 2008-08-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Ehd1 and OsMADS51 in rice play a role in promoting flowering under short-day conditions, but their homologous genes do not exist in Arabidopsis; and the key regulatory gene FLC in the autonomous flowering pathway of Arabidopsis is not found in rice. Homologous gene (Doi et al., Ehd1, a B type responder regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1, Genes Dev (2004): 926-936; Kim et al., OsMADS51 is a short -day flowering promoter that functions upstream of Ehd1, OsMADS14, and Hd3a, Plant Physiol (2007): 145: 1484-94; Michaels et al., FLOWERING LOCUSC encodes a novel MADS domain protein that acts as a repressor of flowering, Plant Cell (1999) :949-56)

Method used

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  • Clone of gene RID1 for controlling rice floral conversion and heading stage and application thereof
  • Clone of gene RID1 for controlling rice floral conversion and heading stage and application thereof
  • Clone of gene RID1 for controlling rice floral conversion and heading stage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation and cloning of RID1 gene

[0028] 1. Obtaining non-heading mutants

[0029] The mutants identified in the present invention are from rice T-DNA insertion mutants (the creation method of the mutant library has published papers, see Wu et al., Development of enhancer trap lines for functional analysis of the rice genome, Plant J (2003): 418-427; Zhang et al., Non-random distribution of T-DNA insertions at various levels of the genome hierarchy as revealed by analyzing 13, 804 T-DNA flanking sequences from an enhancer-trap mutant library, Plant J(2007): 947- 959). The screening identification method of mutant is: from above-mentioned rice T-DNA insertion mutant library (see http: / / rmd.ncpgr.cn / ), the T0 transgenic rice seeds were planted in the field after conventional soaking and germination procedures to obtain each T 1 A total of 20 plants were planted in the generation family. The plants were planted in the experimental field of Huazhong Agricu...

Embodiment 2

[0038] Example 2: RID1 gene is expressed in immature leaves and localized in the nucleus

[0039] The present invention at first utilizes semi-quantitative RT-PCR to analyze the expression pattern of RID1 gene, plant tissue total RNA sample extraction, reverse transcription and RT-PCR reaction conditions see references (Huang etc., Down-Regulation of a SILENT INFORMATION REGULATOR2-RelatedHistone Deacetylase Gene, OsSRT1, Induces DNA Fragmentation and Cell Death in Rice, Plant Physiol (2007): 1508-1519). The total RNA was obtained from the plant tissues of the wild-type rice variety "Zhonghua 11" and the rid1 mutant grown under artificially controlled diurnal neutral conditions (12 hours day / 12 hours night): leaves and leaf sheaths 35 days after germination Total RNA was extracted from , immature leaves, roots, shoot apex tissues and flower organ meristems 41 days after germination. RT-PCR results showed that the RID1 gene was only expressed at a very low level in the immatur...

Embodiment 3

[0045] Example 3: RID1 regulates known heading date-related regulatory genes

[0046] In order to analyze the molecular mechanism of RID1 gene regulating rice flowering, the present invention uses quantitative RT-PCR technology to detect the expression of related heading date regulation genes in rid1 mutants and wild-type plants. The results are shown in Figure 4, no matter in two consecutive days under long-day conditions (14 hours day / 10 hours night) or short-day conditions (10 hours day / 14 hours night), compared with the expression in wild-type rice plants, The expression of Ehd1 and Hd3a in the rid1 mutant was severely suppressed, almost undetectable; while the expression of Hd1 gene in the rid1 mutant was lower than that of the wild-type plant during the day, but the difference was not obvious at night. This shows that the RID1 gene mainly affects the flowering of rice by regulating the expression of Ehd1 and Hd3a. The study also found that the expression of the RID1 gen...

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Abstract

The invention relates to the technical field of plant gene engineering, which particularly relates to an isolation and cloning, functional verification and application of gene RIDI which regulates and controls plant(rice) flower formation conversion or / and ear sprouting period. RID1 gene which is cloned by the invention is tested to have the function of regulating and controlling plant (rice) flower formation conversion or / and ear sprouting period through a gene function-complement experiment. Gens and encoding protein which are cloned by the invention can be widely applied in regulating and controlling flowering characteristics of plants and improving child-bearing age of plant varieties and area adaptation range, which has an important application value.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering. Specifically, it relates to the cloning, functional verification and application of a gene RID1 for controlling plant (rice) flowering transition or / and heading date. Transforming the gene into the rice plant can start the flowering conversion process of the rice plant and regulate the heading date of the rice. Background technique [0002] Flowering (shown as heading in rice) is an important growth and development process for plants to transition from vegetative growth to reproductive growth. The timing of plant flowering is important, especially when the desired product is flowers or seeds derived therefrom, and in order for plants to reproduce sexually successfully, they must flower under suitable environmental conditions. For example, common wild rice is mainly distributed in tropical regions such as southern China and eastern India, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415
Inventor 吴昌银游常军李才顺张启发
Owner HUAZHONG AGRI UNIV
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