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Method for producing feeding lactobacillus acidophilus by solid-liquid two-step fermentation technique

A Lactobacillus acidophilus and two-step fermentation technology, applied in the field of producing Lactobacillus acidophilus for feed, can solve the problems of low fermentation level, inability to achieve the application effect of feed industry, high production cost, and achieve fast growth and reproduction and production cost. Low, high-yield effects

Active Publication Date: 2008-08-27
哈尔滨大蓝饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to aim at the defects existing in the current fermentation production of Lactobacillus acidophilus, or the effect is good but the production cost is too high, or the cost is not high but the fermentation level is too low, and the application effect of the feed industry cannot be reached. Provide a method for producing Lactobacillus acidophilus for feeding, which uses cheap and readily available agricultural and sideline products as raw materials, supplemented by a reasonable fermentation process, to improve the fermentation level and reduce production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: (production Lactobacillus acidophilus method 1 for feeding)

[0026] Concrete process of the present invention is as follows:

[0027] (1) Culture of Candida lipolytica (Yarrowia lipolytica):

[0028] ① Liquid test tube seed culture:

[0029] Using 10°Bx saccharification wort culture medium: take a certain amount of barley malt, crush it, add 60°C water 4 times the weight of the malt, keep warm and saccharify at 55-60°C, stir constantly, after 3-4 hours, filter with gauze , remove the residue, filter once with filter paper after boiling to obtain clarified wort, add water to dilute it into 10°Bx wort; after sterilizing the test tube liquid wort medium at 121°C for 20 minutes, pick a little preserved lipolytic fake Inoculate the original strain of Trichotilpha cerevisiae into the wort medium, culture it statically at 28-30°C for 24-30 hours, store it at 0-4°C, and keep it for later use;

[0030] ②Seed culture in triangular flask:

[0031] Fill each 500m...

Embodiment 2

[0043] Embodiment 2: (production feed Lactobacillus acidophilus method 2)

[0044] In this example, (1) the strain culture of Candida lipolytica (Yarrowia lipolytica): 2. triangular flask seed culture: in the triangular flask of each 500ml, fill into by bran, corn flour and diammonium hydrogen phosphate by weight 80:25:0.8 ratio of 25 grams of solid matter, 1.3 times the weight of the solid matter, add 32.5ml of water;

[0045] (2) Solid fermentation culture of Candida lipolytica: The solid fermentation medium is prepared from soybean meal, bran, corn flour and diammonium hydrogen phosphate in a ratio of 45:45:20:0.8 by weight, and after adding equal weight water , sterilized at 0.1Mpa for 30min, add 200ml sterile water to the seeds in step (1) ②, after fully stirring, inoculate according to the ratio of each bottle of seeds: 15 kg of solid fermentation medium based on the weight of solids, and the fermented material The thickness is 2.5cm;

[0046] (3) Bacterial culture of ...

Embodiment 3

[0050] Embodiment 3: (production feed Lactobacillus acidophilus method 3)

[0051] In this example, (1) the strain culture of Candida lipolytica (Yarrowia lipolytica): 2. triangular flask seed culture: in the triangular flask of each 500ml, fill into by bran, corn flour and diammonium hydrogen phosphate by weight 90:20:1 ratio of 30 grams of solid matter, and 30ml of water is added to double the weight of the solid matter;

[0052] (2) Solid fermentation culture of Candida lipolytica: The solid fermentation medium is prepared from soybean meal, bran, corn flour and diammonium hydrogen phosphate in a ratio of 60:30:30:1 by weight, and after adding equal weight water , 0.1Mpa sterilized for 30min, step (1) ② add 300ml sterile water to each triangular bottle of seeds, after fully stirring, inoculate according to the ratio of each bottle of seeds: 20 kg of solid fermentation medium based on the weight of solids, and the fermented material The thickness is 3cm;

[0053] (3) Bacte...

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Abstract

The invention discloses a method for adopting a solid-liquid two-step fermentation technique to produce feeding lactobacillus acidophilus, which belongs to the microorganism technical field. The method comprises: firstly, culturing a strain of yarrowia lipolytica, secondly, fermenting and culturing solid of the yarrowia lipolytica, thirdly, culturing the strain of lactobacillus acidophilus, fourthly, fermenting and culturing liquid of the lactobacillus acidophilus, fifthly, absorbing and packaging lactobacillus acidophilus fermentation broth, and the like. The method of the invention adopts cheap and easily obtained agricultural byproducts such as soybean meal, wheat bran, corn meal and the like as raw materials to produce the feeding lactobacillus acidophilus through the solid-liquid two-step fermentation, the method has the characteristics of low production cost, rapid growth and reproduction, high yield and the like, and the method overcomes the difficult problem that previous feeding lactobacillus acidophilus has over high production cost and can be extended and applied in livestock and poultry feedstuff production enterprises.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing Lactobacillus acidophilus for feed using a two-step process of solid fermentation of Candida lipolytica and liquid fermentation of Lactobacillus acidophilus. Background technique [0002] Live microbial preparations for feeding can maintain and adjust the micro-ecological balance in the intestinal tract of animals, and directly or indirectly interfere, eliminate and antagonize pathogenic bacteria, thereby improving disease resistance and improving animal production levels. Because it has no drug resistance, no residue, and has the effect of improving animal production performance and preventing diseases, it can overcome some adverse consequences caused by long-term and large-scale use of antibiotics. It is considered to be potential, natural, and harmless to the environment and health of antibiotics. substitution. Lactobacillus acidophilus h...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16A23K1/16C12R1/23C12R1/73A23K10/18
Inventor 李孝辉叶琪明陈兵许尧兴李艳丽许少春
Owner 哈尔滨大蓝饲料有限公司
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