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Method for preparing liquid chromatography of monascin and monascus anka flavine pure product

Anka monascus xanthin and liquid chromatography technology, applied in the field of preparation of pure pigment products, can solve the problems of inability to collect single-component isolates, low purity of separated components, poor repeatability, etc., and achieve mechanization The effect of high degree, large amount of preparation, simple process and operation process

Inactive Publication Date: 2010-06-16
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

Conventional chromatographic techniques, such as thin-layer chromatography and column chromatography, have defects such as complex operation, low purity of separated components, and poor repeatability. Gas chromatography can only separate and analyze gasification or have sufficient vapor pressure at the analysis temperature. Generally, analytical liquid chromatography is mainly suitable for the separation and analysis of mixtures. Due to the small injection volume, it is impossible to collect a large number of single-component isolates.
[0003] Therefore, for the preparation of single-component pure products, the above conventional chromatographic separation techniques have certain limitations.

Method used

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  • Method for preparing liquid chromatography of monascin and monascus anka flavine pure product
  • Method for preparing liquid chromatography of monascin and monascus anka flavine pure product
  • Method for preparing liquid chromatography of monascin and monascus anka flavine pure product

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Embodiment Construction

[0019] Example—Using red yeast rice powder with a color value of 800 to prepare pure products of monascus and Anka monascus xanthin

[0020] (1) Weigh 10 g of red yeast rice powder and put it into a Soxhlet extractor, add n-hexane to reflux at 80°C for extraction until the extract is colorless, collect the extract and filter it through filter paper, concentrate under reduced pressure to form a paste, and obtain a crude extract of monascus pigment.

[0021] (2) Redissolve the crude extract in methanol, bring to a 50ml volumetric flask to volume, and filter through a 0.45μm filter membrane to obtain a sample solution.

[0022] (3) Open the preparative liquid phase instrument, set the chromatographic conditions (C18 chromatographic column, column temperature is room temperature, detection wavelength 233nm), prepare methanol: water (8: 2) mobile phase, ultrasonic degassing, flush the chromatographic column, stabilize the baseline, The sample solution was manually injected to start...

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Abstract

The liquid chromatography preparation method for pure products of monascin and ankaflavine belongs to a preparing method for pure products, which uses preparative liquid chromatograph, C18 chromatographic column, wherein the column temperature is room temperature, to detect the target components of which the wave length is 233nm or 394nm, the ratio of mobile phase methanol methanol and water is 8:2, the feeding amount for sample is 1ml, the flow speed is 5ml / min, and the collecting retention times are 37. 733min (monascin) and 68. 100min (ankaflavine) respectively, while the continuous production can be realized by matching the equipment with automatic sampling feeder, automatic collector. The technology can be applied to monascus pigment products with monascin and ankaflavine such as monascus rice flour, monascus pigment powder pasted monascus pigment. The preparing process is simple, convenient in operation, rapid and highly effective, has high automaticity, and can prepare the pureproducts of monascin and ankaflavine at the same time with high product purity and big preparing amount.

Description

technical field [0001] The invention relates to a preparation method of pure pigments, in particular to a liquid chromatography preparation technology for pure pigments, which is suitable for the liquid chromatography separation and preparation of monascus and anca-monascus xanthin in monascus pigments. Background technique [0002] In recent years, with the improvement of people's food safety requirements and the increasing demand for various pure products in analysis, testing and quality control, the research on the preparation technology of various single-component pure products has become more and more extensive. Chromatography can separate compounds with little difference in properties and is an important method for separating different components from a mixture. Conventional chromatographic techniques, such as thin-layer chromatography and column chromatography, have defects such as complex operation, low purity of separated components, and poor repeatability. Gas chro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/04B01D15/30
Inventor 张德权崔莉胡晓丹李淑荣李庆鹏
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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