Micrococcus luteus with novel metabolic characteristic and application thereof in biological degradation
A technology for the metabolic characteristics of Micrococcus luteus, applied in the aerobic biodegradation of picolinic acid, in the field of Micrococcus luteus, can solve the problems of non-degradability, achieve the effect of enhancing processing capacity and broad application potential
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0029] Example 1
[0030] The screening steps of Micrococcus luteus provided by the present invention are as follows:
[0031] Samples of aerobic activated sludge were collected from the aeration tank of the Dalian Dyestuff Factory as a source of bacteria for domestication and cultivation. Add 0.2% sodium pyrophosphate as a deflocculant to 50ml of activated sludge, shake on a shaker for 5-10 minutes to break up the activated sludge flocs, take 5ml of activated sludge and add 95ml of inorganic salt medium to shake In the bottle, add nitrobenzene as the only carbon, nitrogen, and energy source. The concentration of nitrobenzene is 200mg / L. The culture is shaken at 30°C and the rotation speed is 180r / min. Inorganic salt medium composition: Na 2 HPO 4 ·12H 2 O, 7g / L; KH 2 PO 4 , 1g / L; CaCl 2 ·2H 2 O, 10mg / L; FeCl 3 , 2mg / L; MgSO 4 ·7H 2 O, 20mg / L. When the bacterial liquid becomes turbid and nitrobenzene cannot be detected, proceed to the next transfer; after continuous domestication ...
Example Embodiment
[0047] Example 2
[0048] In the present invention, the application of Micrococcus luteus to the study of aerobic degradation of nitrobenzene has the following steps:
[0049] [1] Add 100ml of inorganic salt culture medium to a 250ml Erlenmeyer flask, then add 200mg / L of nitrobenzene as the only carbon, nitrogen and energy source, sterilize at 121℃;
[0050] [2] The liquid culture of Micrococcus luteus prepared in Example 1 was added to the medium of [1], 30°C, 180r / min, aerobic culture, figure 1 It is the growth of Micrococcus luteus and the degradation of nitrobenzene.
Example Embodiment
[0051] Example 3
[0052] In the present invention, the application of Micrococcus luteus to the study of aerobic degradation of picolinic acid is as follows:
[0053] [1] Add 100ml of inorganic salt culture medium to a 250ml Erlenmeyer flask, add 2000mg / L picolinic acid as the only carbon, nitrogen and energy source, sterilize at 121℃;
[0054] [2] The liquid culture of Micrococcus luteus prepared in Example 1 was added to the medium of [1], 30°C, 180r / min, aerobic culture, figure 2 It is the growth of Micrococcus luteus and the degradation of picolinic acid.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap