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Enzyme producing method for white rot fungus

A technology of white rot fungi and cells, applied in the field of environmental biology, can solve the problems of ecological damage to human health, strong toxicity, complex components, etc., and achieve the effect of continuous high-efficiency output and reduced cost of enzyme production.

Inactive Publication Date: 2008-09-10
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
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Problems solved by technology

[0002] Refractory organic industrial wastewater, including dye chemical wastewater, has the characteristics of high chroma, high salinity, strong toxicity, high pollutant content and complex components, and is difficult to degrade. It is the focus and difficulty of industrial wastewater pollution control. These industrial wastewater If it is discharged into the environment without proper treatment, it will cause serious ecological damage and human health problems.

Method used

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Embodiment Construction

[0012] Below in conjunction with embodiment the present invention is described in detail:

[0013] (1) Pretreatment, size, feeding amount and dosing time of corncobs: Wash the corncobs with clean water first, and then process them into cubes with a size of 0.5cm×0.5cm×0.5cm. Sterilize under Mpa for 15 minutes, put it in a sterile room and let it cool down, then put it in a shaker flask filled with nitrogen-limited medium in a sterile room, so that the number of corncob carriers in each shaker bottle remains at 10 / 100ml.

[0014] (2) Preparation of white rot fungus spore liquid: inject a small amount of sterile water into the plate (laboratory sterile water: add 100mL water into a 200mL or 250mL triangular flask, add a cotton plug, bandage, sterilize at 121°C, 0.1Mpa for 30 Minutes), scrape off the spore powder with a push rod, and filter through 4 layers of lens paper to filter out the mycelium. The resulting filtrate was counted with a hemocytometer.

[0015] Bacteria cou...

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Abstract

The invention relates to a white rot fungi continuous high-efficient enzyme producing method, which belongs to the environmental biotechnical field and can be applied in the condition of difficult degradation of organic waste water treatment and environmental repair. In order to realize the aims of the invention, the white rot fungi continuous high-efficient enzyme producing method uses cobs as growth carriers and supplemental nutrition substrates of white rot fungus, utilizes an air-lift fermentation apparatus and optimizes the fermentation conditions, realizes continuous high-efficient output of lignin peroxidases and manganese peroxidases, and simultaneously reduces the enzyme production cost.

Description

technical field [0001] The patent of the present invention relates to a method for continuous and high-efficiency enzyme production by white-rot fungi, which belongs to the field of environmental biotechnology and can be applied to refractory organic wastewater treatment and environmental restoration. Background technique [0002] Refractory organic industrial wastewater, including dye chemical wastewater, has the characteristics of high chroma, high salinity, strong toxicity, high pollutant content and complex components, and is difficult to degrade. It is the focus and difficulty of industrial wastewater pollution control. These industrial wastewater If it is discharged into the environment without proper treatment, it will cause serious ecological damage and human health problems. [0003] Under the catalysis of lignin degrading enzymes, white rot fungi can degrade various organic pollutants in dye wastewater with broad spectrum and high efficiency through free radical ch...

Claims

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Application Information

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IPC IPC(8): C12N9/08C02F3/00C12R1/645
Inventor 黄民生高尚陶芳
Owner EAST CHINA NORMAL UNIV
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