Mixed fermentation enzyme production process

A technology of mixed fermentation and process, applied in the field of fermentation, can solve problems such as environmental pollution, achieve the effect of low price and reduce the cost of enzyme production

Active Publication Date: 2021-03-05
江阴唯农生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Corn stalks belong to agricultural waste, and most of them are discarded or burned at will. However, incineration will cause environmental pollution and has been banned by the state. At present, many companies are studying the utilization methods of corn stalks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of mixed fermentation enzyme production technology, it comprises the steps:

[0036] Preparation of Trichoderma reesei seed liquid: Inoculate Trichoderma reesei on PDA solid medium for cultivation to obtain a single colony; pick a single colony and inoculate it into PDA liquid medium for seed cultivation to obtain a concentration of 2 × 10 8 The Trichoderma reesei seed liquid of cfu / ml;

[0037] Preparation of Rhizopus stoloniferus seed solution: Inoculate Rhizopus stoloniferus on PDA solid medium and cultivate to obtain a single colony; pick a single colony and inoculate it on the seed medium for seed culture, and obtain a concentration of 5 × 10 8 The rhizopus stolonifera seed liquid of cfu / ml; The component of described seed culture medium is: glucose 1.5%, ammonium sulfate 0.4%, potassium dihydrogen phosphate 0.6%, corn steep liquor 2%, surplus is water, above is weight percentage;

[0038]Preparation of Bacillus pumilus seed solution: Inoculate Bacillus pu...

Embodiment 2

[0042] A kind of mixed fermentation enzyme production technology, it comprises the steps:

[0043] Preparation of Trichoderma reesei seed liquid: Inoculate Trichoderma reesei on PDA solid medium for cultivation to obtain a single colony; pick a single colony and inoculate it into PDA liquid medium for seed cultivation to obtain a concentration of 2 × 10 8 The Trichoderma reesei seed liquid of cfu / ml;

[0044] Preparation of Rhizopus stoloniferus seed solution: Inoculate Rhizopus stoloniferus on PDA solid medium and cultivate to obtain a single colony; pick a single colony and inoculate it on the seed medium for seed culture, and obtain a concentration of 5 × 10 8 The rhizopus stolonifera seed liquid of cfu / ml; The component of described seed culture medium is: glucose 1.5%, ammonium sulfate 0.4%, potassium dihydrogen phosphate 0.6%, corn steep liquor 2%, surplus is water, above is weight percentage;

[0045] Preparation of Bacillus pumilus seed solution: Inoculate Bacillus p...

Embodiment 3

[0049] Effects of compatibility of different strains on enzyme activity:

[0050] The invention adopts the conventional 3,5-dinitrosalicylic acid method to detect the activity of xylanase and cellulase.

[0051] Group settings:

[0052] Experimental group: Example 1;

[0053] Control group 1: Simultaneous vaccination, the rest are the same as in Example 1;

[0054] Matched group 2: Trichoderma reesei+rhizoopsis stolonifer, all the other are with embodiment 1;

[0055] Control group 3: Trichoderma reesei+Bacillus pumilus, all the other are the same as embodiment 1;

[0056] Control group 4: Rhizopus stolonifer+Bacillus pumilus, all the other are with embodiment 1;

[0057] like figure 1 As shown, the fermentation enzyme activity of the experimental group was the highest, the xylanase activity reached 288U / ml, and the cellulase activity reached 206U / ml, which was significantly higher than that of the control group 1 inoculated at the same time, and the control group using t...

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Abstract

The invention belongs to the technical field of fermentation, and discloses a mixed fermentation enzyme production process. The process comprises the following steps: inoculating a trichoderma reeseiseed liquid into a fermentation tank according to an inoculation amount of 5-10%, performing culturing for 12 h, then inoculating a rhizopus stolonifer seed liquid and a bacillus pumilus seed liquid according to an inoculation amount of 5-10%, continuously performing culturing for 12 h, then adding a corn straw powder, and continuously performing culturing for 48-60 h to obtain fermentation liquor. According to the process, a strain mixed fermentation enzyme production mode is adopted, the synergistic effect is good, and the enzyme production efficiency is high.

Description

[0001] cross reference [0002] This application claims the priority of the Chinese patent application No. 2018108823397 filed on August 6, 2018 with the title of "A Mixed Fermentation Enzyme Production Process", the entire disclosure content of which is cited herein. technical field [0003] The invention belongs to the technical field of fermentation and relates to a process for producing enzymes by mixed fermentation, in particular to a process for producing xylanase and cellulase by mixed fermentation. Background technique [0004] Xylanase is a general term for a group of enzymes that can degrade hemicellulose into oligosaccharides and xylose. Xylanase has a wide range of applications in the fields of feed, food, paper, textile, medicine and energy. Xylanase is used as a feed enzyme preparation, which can effectively relieve the anti-nutritional effect of xylan and promote the digestion and absorption of roughage by livestock and poultry. As a new pulp bleaching aid, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/24C12R1/885C12R1/845C12R1/07
CPCC12N9/2437C12N9/248Y02E50/10
Inventor 胡叶飞金如张宏杰金荣培
Owner 江阴唯农生物科技有限公司
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