Method for extracting hesperidin and naringin

An extraction method and naringin technology, applied in the field of analytical chemistry, can solve the problems of unfavorable content analysis and determination, large amount of organic solvent, low extraction efficiency, etc., and achieve short extraction time, less sample amount, and good reproducibility. Effect

Inactive Publication Date: 2008-09-17
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the methods for extracting hesperidin and naringin that have been reported are only organic solvent extraction or ultrasonic extraction, and the method has the following defects: the amount of organic solvent is large, ...

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  • Method for extracting hesperidin and naringin
  • Method for extracting hesperidin and naringin
  • Method for extracting hesperidin and naringin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 MAE-HPLC analysis of the content of hesperidin in different brands of orange juice

[0039] Instruments and materials:

[0040] Instrument: Shimadzu CL-10 liquid chromatograph, including SPD-10A ultraviolet detector and CTO-10A column thermostat, 20μL manual injection system, chromatography workstation, LWMC-205 adjustable power microwave chemical reactor, Millipore Direct-Q pure water generator.

[0041] Materials: Orange juice drinks of various brands. Methanol (Merck, chromatographically pure), trifluoroacetic acid and dimethyl sulfoxide were all analytically pure, deionized pure water was produced by Millipore Direct-Q pure water generator, and the standard hesperidin was obtained from China National Institute for the Control of Pharmaceutical and Biological Products .

[0042] Sample pretreatment:

[0043] Take 1ml of fruit juice each in a 50ml round bottom flask, add 5ml of 50% DMSO aqueous solution, shake, and under the condition of power of 500W, a...

Embodiment 2

[0048] Example 2 MAE-HPLC analysis of the contents of hesperidin and naringin in grapefruit juice

[0049] Instruments and materials:

[0050] Instrument: Shimadzu CL-10 liquid chromatograph, including SPD-10A ultraviolet detector and CTO-10A column thermostat, 20μL manual injection system, chromatography workstation, LWMC-205 adjustable power microwave chemical reactor, Millipore Direct-Q pure water generator.

[0051] Materials: 2 brands of grapefruit and orange juice mix drinks. Methanol (Merck, chromatographically pure), trifluoroacetic acid and dimethyl sulfoxide were all analytically pure, deionized pure water was produced by Millipore Direct-Q pure water generator, and the standard hesperidin was obtained from China National Institute for the Control of Pharmaceutical and Biological Products .

[0052] Sample pretreatment:

[0053] Take 1ml of fruit juice each in a 50ml round bottom flask, add 5ml of 50% DMSO aqueous solution, and shake. Under the condition of a po...

Embodiment 3

[0054] Example 3 MAE analysis of the content of hesperidin in the Chinese herbal medicine tangerine peel

[0055] Instruments and materials:

[0056] Instrument: Shimadzu CL-10 liquid chromatograph, including SPD-10A ultraviolet detector and CTO-10A column thermostat, 20μL manual injection system, chromatography workstation, LWMC-205 adjustable power microwave chemical reactor, Millipore Direct-Q pure water generator.

[0057] Materials: traditional Chinese medicine tangerine peel (origin: Shanghai), methanol (Merck, chromatographically pure), trifluoroacetic acid and dimethyl sulfoxide are analytically pure, deionized pure water is made by Millipore Direct-Q pure water generator, orange peel Glycoside standard products come from China National Institute for the Control of Pharmaceutical and Biological Products (purity>99%).

[0058] Sample pretreatment:

[0059] Accurately weigh about 1g of tangerine peel powder, put it in a 50ml round bottom flask, add 10ml of 50% DMSO aq...

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Abstract

The invention relates to a method for extracting hesperidin and naringin by microwave-assisted extraction (MAE), which belongs to the field of analytical chemistry. The method includes quantitatively weighing sample, extracting hesperidin and naringin from the sample in mixed solvent of water and organic solvent in a microwave-assisted extraction device by selecting microwave power and extraction time through MAE, centrifuging, collecting the supernatant, diluting, and analyzing and measuring the content of hesperidin and naringin by HPLC. The method is simple and rapid, and has less sample usage amount, good repeatability, and high recovery rate. The method in combination with the HPLC can be used for accurately measuring the content of hesperidin and naringin in fruit juice, fresh fruit and Chinese traditional medicine, and evaluating quality thereof.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for extracting hesperidin and naringin, in particular to a method for extracting hesperidin and naringin by microwave-assisted extraction technology. Background technique [0002] Hesperidin (Hesperidin) is a kind of raw material extracted from Citrus aurantium, which belongs to vitamin medicine, can reduce the fragility and permeability of capillaries, and is used for the auxiliary treatment of hypertension and capillary hemorrhagic diseases. Reduced capillary resistance has the effect of improving (enhancing the effect of vitamin C), has anti-inflammatory and anti-viral effects, and is a drug for treating hypertension and myocardial infarction. One of the components; Naringin (Naringin) is a dihydroflavone compound. Naringin shows a variety of biological activities and pharmacological effects. It has antibacterial, anti-inflammatory, anti-cancer, antispasmodic and ch...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/06
Inventor 段更利郁颖佳李晓雯干志彬
Owner FUDAN UNIV
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