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Method for extracting lentinan by using discarded mushroom culture medium as raw material

A technology of shiitake mushroom culture medium and lentinan, which is applied in the field of comprehensive utilization of waste shiitake mushroom culture medium to extract lentinan, can solve the problems of high crude fiber content in the culture medium and difficulty in extraction, and achieve reduced production costs, less equipment investment, and reduced sewage discharge Effect

Inactive Publication Date: 2008-09-17
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the shiitake mushrooms are harvested, there are still a large number of hyphae remaining in the culture medium. Due to the high content of crude fiber in the culture medium, it is difficult to extract them, so they are all discarded. According to research, there is still a high content of lentinan in the shiitake mushroom culture medium. There is currently no report on the extraction of lentinan from the waste culture medium of Lentinus edodes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add 100 grams of shiitake mushroom medium powder with a water content of 3% to 5% and a particle size of 60 mesh into 2000 grams of 80% ethanol. After degreasing for 150 minutes, the ethanol solution and the mushroom medium powder are centrifuged and dried.

[0019] Add the mushroom medium powder to 6000 g of water, boil at 100° C. for 1 hour, and filter to obtain the filtrate.

[0020] Vacuum concentration is adopted, the vacuum degree can be 0.075Mpa, the temperature is 65°C, and the extraction solution is concentrated to 1 / 2 of the original volume.

[0021] Add 95% ethanol to the leaching concentrate so that the mass percent concentration of ethanol in the mixed solution is controlled at 80%, centrifuge after 6 hours of precipitation (the speed is 7500r / min, and the centrifugation time is 5min), and then the precipitate is placed at 80°C Drying at a lower temperature to obtain lentinan powder.

Embodiment 2

[0023] Add 150 grams of shiitake mushroom medium powder with a water content of 3% to 5% and a particle size of 40 meshes into 3000 grams of 95% ethanol. After degreasing for 120 minutes, centrifuge the ethanol solution and the mushroom medium powder, and dry them.

[0024] Add the mushroom medium powder to 4500 g of water, extract at 90° C. for 4 hours, and filter to obtain the filtrate.

[0025] Vacuum concentration is adopted, the vacuum degree can be 0.095Mpa, the temperature is 65°C, and the extraction solution is concentrated to 1 / 5 of the original volume.

[0026] Add 90% ethanol to the leaching concentrate so that the mass percent concentration of ethanol in the mixed solution is controlled at 60%, centrifuge after 12 hours of precipitation (the speed is 5500r / min, and the centrifugation time is 15min), and then the precipitate is placed at 60°C Drying at a lower temperature to obtain lentinan powder.

Embodiment 3

[0028] Add 200 grams of shiitake mushroom medium powder with a water content of 3% to 5% and a particle size of 80 mesh into 2000 grams of 90% ethanol, and after defatting for 180 minutes, centrifuge the ethanol solution and the mushroom medium powder, and dry them.

[0029] Add the mushroom medium powder into 2000 g of water, extract at 60° C. for 8 hours, and filter to obtain the filtrate.

[0030] Vacuum concentration is adopted, the vacuum degree can be 0.075Mpa, the temperature is 85°C, and the extraction solution is concentrated to 1 / 5 of the original volume.

[0031] Add 95% ethanol to the leaching concentrate so that the ethanol mass percentage concentration in the mixed solution is controlled at 80%, centrifuge after 18 hours of precipitation (the speed is 4000r / min, and the centrifugation time is 20min), and then the precipitate is placed at 60°C Drying at a lower temperature to obtain lentinan powder.

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Abstract

The invention relates to a method for extracting lentinan by an abandoned mushroom culture medium, which belongs to the technology field of refined and deep processing of agricultural and sideline products and the comprehensive utilization of the by-products thereof. The preparation method comprises pretreatment of smashing and skimming of raw materials, thermal reflux extraction, concentration, ethanol precipitation separation and drying process. The skimmed mushroom culture medium powder adopts the thermal reflux extraction technology to obtain the lentinan crude extract with the yield of 4.78 percent. The process route designed by the invention is simple, the equipment investment is less, the industrial popularization is feasible, which can not only change waste into wealth, but can also greatly reduce the cost for preparing the lentinan; furthermore, the obtained lentinan can be prepared into oral liquor, capsules, chewable tablets or buccal tablets and other various edible preparations by adding or not adding other components, thus opening a novel way for comprehensively utilizing the abandoned mushroom culture medium and reducing the pollution discharge.

Description

technical field [0001] The invention relates to a method for extracting lentinan polysaccharide through comprehensive utilization of discarded mushroom medium, and belongs to the technical field of deep processing of agricultural byproducts and comprehensive utilization of byproducts. Background technique [0002] Lentinus edodes is a traditional Chinese medicinal material in my country, which contains a variety of active ingredients. Modern research results show that lentinan has various pharmacological activities such as inhibiting tumors, improving immune function, anti-virus and anti-oxidation, and has no toxic side effects. Because shiitake mushrooms contain two medicinal physiologically active substances, lentinan, which has the functions of anti-virus, anti-tumor, regulating immune function and stimulating interferon formation, and water-soluble lignin, which can enhance human immunity, it has attracted widespread attention. [0003] At present, the industrialized lar...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘静波林松毅
Owner JILIN UNIV
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