Preparation method for enriching gamma-aminobutyric acid by using fixed rice bran glutamic acid decarboxylase

A technology of glutamic acid decarboxylase and aminobutyric acid, applied in the direction of fixing on/in organic carrier, fermentation, etc., can solve problems such as waste, achieve the effect of less impurity content, wide application range, and scientific and reasonable design

Inactive Publication Date: 2008-09-17
JIANGNAN UNIV
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the rice bran resources in my country are consumed in the form of traditional feed, which is very wasteful.
[0003] Various foods with high GABA content are now sold in the market in Japan, such as germinated brown rice,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for enriching gamma-aminobutyric acid by using fixed rice bran glutamic acid decarboxylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 20g of sodium alginate and dissolve it in 1L of deionized water, stir and dissolve evenly to obtain a sodium alginate solution, and the weight of the solution is taken as a part of the total weight of the immobilized enzyme; the raw rice bran is crushed through a 60-mesh sieve, and 60g of sieved rice bran is weighed and dissolved Mix and stir the good sodium alginate solution evenly, put it into a ball maker, and drop 1.5% CaCl at a speed of 1 drop / s at a distance of 10cm from the liquid surface 2 solution (with CaCl 2 The container tool stirring device of solution, stirring speed is advisable that the spheroid in the solution does not sink to the bottom), making balls. When the ball making is over, make the pellets in CaCl 2 The solution was stirred and suspended for 1h to harden. CaCl was removed after 1h 2 solution, added with CaCl 2 Adjust the same amount of deionized water as the solution and continue to stir for 20 minutes. After 20 minutes, change to fr...

Embodiment 2

[0026] Weigh 10g of sodium alginate and dissolve it in 0.5L deionized water, stir and dissolve evenly to obtain a sodium alginate solution, the weight of the solution is taken as part of the total weight of the immobilized enzyme; the raw material rice bran is crushed through a 60-mesh sieve and weighed 30g and the dissolved seaweed Mix and stir the sodium chloride solution evenly, put it into a ball maker, and drop 1% CaCl at a speed of 1 drop / s at a distance of 10cm from the liquid surface 2 solution (contains CaCl 2 The container tool stirring device of solution, stirring speed is advisable with the spheroid in the solution not sinking to the bottom), making balls. When the ball making is over, make the pellets in CaCl 2 The solution was stirred and suspended for 1h to harden. CaCl was removed after 1h 2 solution, added with CaCl 2 Adjust the same amount of deionized water as the solution and continue to stir for 20 minutes. After 20 minutes, change to fresh deionized w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of enriching GABA by immobilized rice bran glutamate decarboxylase, and belongs to the technology field of the deep processing of the rice bran. In the method, the rice bran which is byproducts obtained after paddies are processed is used as raw material; the rice bran is mixed with sodium alginate solution with certain concentration and evenly stirred after being crushed and screened, and then is put in a balling device; CaCl2 solution is dropwise dropped into the mixed solution to act spherulization; ball bodies are sclerotized; the redundant CaCl2 and the rice bran which is not immobilized are washed, so that the immobilized rice bran glutamate decarboxylase is obtained; the immobilized rice bran glutamate decarboxylase is mixed with PBS buffer solution, sodium glutamate is added in and used as zymolyte, the concentration of the sodium glutamate is 0.01 to 0.05mol/l, the mixed solution reacts in a table concentrator under the temperature of 45 DEG C to obtain GABA enriched solution, and products are obtained after being dried. The protein content and the sugar content thereof are only a tenth part of the products which are obtained by adopting a traditional method of enriching the GABA by directly utilizing the rice bran; the products obtained by the method can be used as functional food additives; the efficacies of lowering blood pressure, calming, aiding sleeping, protecting liver, etc. can be realized; the preparation method has scientific and reasonable design, and has great significance to realize the comprehensive utilization of rice bran resources and increase the added value thereof.

Description

technical field [0001] The invention discloses a preparation method for enriching gamma-aminobutyric acid (GABA) by immobilized rice bran glutamic acid decarboxylase, which belongs to the technical field of rice bran deep processing. Background technique [0002] Rice is one of the main food crops on the earth. China has always been known as the "Kingdom of Rice". The annual rice output accounts for about 35% of the world's total rice output, ranking first in the world. As a by-product of rice, rice bran has a very rich source, and if it is well utilized, it can achieve high added value. The activity of glutamate decarboxylase in rice bran is very high, which can be used as a catalyst to catalyze the decarboxylation reaction of monosodium glutamate to generate GABA. However, the vast majority of rice bran resources in my country are consumed in the form of traditional feed, which is very wasteful. [0003] Various foods with high GABA content are now sold in the market in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P13/00C12N11/04
Inventor 张晖郭晓娜王立马晓博姚惠源
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap