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Use of modified polysaccharide rich in mannose in preparing medicine for preventing the colitis cancer

A technology of mannose and colitis, applied in the field of application of modified polysaccharides rich in mannose in the preparation of drugs to prevent the canceration of colitis, which can solve problems that have not yet been retrieved

Inactive Publication Date: 2008-11-19
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether dietary fiber can prevent the transformation of ulcerative colitis to colon cancer, there is no research report
No reports similar to this project have been retrieved so far

Method used

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  • Use of modified polysaccharide rich in mannose in preparing medicine for preventing the colitis cancer
  • Use of modified polysaccharide rich in mannose in preparing medicine for preventing the colitis cancer
  • Use of modified polysaccharide rich in mannose in preparing medicine for preventing the colitis cancer

Examples

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preparation example Construction

[0027] The preparation method of the above-mentioned modified polysaccharide rich in galactose is as follows:

[0028] The modified natural gum is hydrolyzed with konjac gum, guar gum and locust bean gum by enzyme (pH=4-5), or by alkali (pH=9-10) and acid (pH=3-5), Or after methanol saponification, alcohol precipitation, dialysis, lyophilization, and then through mannoside enzymatic hydrolysis (pH=4.5-6.5, 37 ° C), to expose the mannose residue, through gel chromatography or molecular weight cut-off, to obtain the target molecular weight (15000 -200000Da) of natural gum is used in pharmaceutical manufacturing. Its specific preparation method is described in detail through Examples 1-4.

[0029] The extraction method of the modified plant polysaccharide is as follows: aloe vera, medlar, shiitake mushroom, black fungus, jujube or rhubarb are pulverized, and after ethanol reflux to remove fat-soluble components, water reflux, ethanol precipitation, dialysis, and freeze-drying to...

Embodiment 1

[0033] Wet 100g of guar gum coarse powder with water, swell in water, dilute with water, reflux for 4 hours (pH=9-10), adjust the pH value to neutral, overnight, then reflux for 2 hours (pH=3-4), Alcohol precipitation, dialysis, concentration, freeze-drying. Take 10 g of denatured guar gum, incubate with mannosidase at 37° C. for 4 hours (pH=4-6), alcohol precipitation, dialysis, concentration, and freeze-drying. Determination of molecular weight, gel chromatography or molecular weight cut-off, obtain guar gum with molecular weight of 15000-200000Da.

Embodiment 2

[0035] Take 100g of konjac gum, after slightly moistening, incubate with mannosidase at 37°C for 4 hours (pH=4-6), adjust the pH value to neutral, overnight, alcohol precipitation, dialysis, concentration, and freeze-drying. Take 10 g of enzymatically hydrolyzed konjac gum, incubate with mannosidase at 37° C. for 4 hours (pH=4-6), alcohol precipitation, dialysis, concentration, and freeze-drying. Determination of molecular weight, gel chromatography or molecular weight cut-off, obtain the modified konjac gum of target molecular weight (15000-200000Da).

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Abstract

The invention relates to the application of a mannose-enriched polysaccharide to drug preparation, in particular to the application of a mannose-enriched modified polysaccharide to the preparation of drugs for preventing colonitis from cancerization. Aiming to make up the blank of the drugs for preventing the inflammatory bowel diseases and especially the ulcerative colitis from cancerization, the application provides the technical proposal that the mannose-enriched modified polysaccharide is applied to the drugs for preventing the colonitis from cancerization, and the modified polysaccharide contains a modified natural gum or a modified plant polysaccharide. The application has the characteristics that a mannose receptor (MR) is taken as a target; the mannose-enriched polysaccharide is separated and extracted from a medicinal plant, and mannosidase is adopted for hydrolyzation; mannose residues are exposed, and regulate gut immunity and relieve the intestinal inflammation after being combined with the MR on the surface of immunological cells, thereby preventing the inflammatory bowel diseases and especially the ulcerative colitis from cancerization. After the mannose-enriched modified polysaccharide is used on a big mouse suffering from the ulcerative colitis, the cancerization inhibitory rate of the ulcerative colitis can reach 93 percent.

Description

technical field [0001] The invention relates to the application of polysaccharide rich in mannose in the preparation of medicine, in particular to the application of the modified polysaccharide rich in mannose in the preparation of medicine for preventing canceration of colitis. Background technique [0002] Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is a type of autoimmune disease related to immune disorders, flora disorders and genetic factors; in European and American countries, the incidence of IBD is as high as 200-300 / 100,000 [1]; in Asia, including my country's Hong Kong and Shanghai, it is showing a rapid upward trend [2]. In recent years, the intake of high-calorie, high-protein, and low-fiber foods has increased significantly in the Chinese population, and the incidence of IBD has increased year by year. At present, there is no specific drug for the treatment of IBD. IBD, especially ulcerative colitis, recurrent attacks and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/736A61P35/00A61P1/00C12P19/04
Inventor 刘莉梅其炳孙阳郭振军
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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