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Process for preparing coenzyme Q10 by sphingosine unit cell strain fermentation, extraction and coupling

A technology for sphingomonas and sphingosine, which is applied in the field of coupling preparation of coenzyme Q10 by using sphingomonas fermentation and extraction, can solve the problems of easy destruction of coenzyme Q, large loss of extraction solvent, time-consuming and labor-intensive and the like. , to achieve the effect of simple and time-saving method, saving manpower and material resources, and improving extraction efficiency

Active Publication Date: 2008-11-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the direct extraction method, the amount of extract obtained by the saponification extraction method is more, but the coenzyme Q 10 Easy to be destroyed and the steps are cumbersome, time-consuming and labor-intensive, and the loss of extraction solvent is large

Method used

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  • Process for preparing coenzyme Q10 by sphingosine unit cell strain fermentation, extraction and coupling
  • Process for preparing coenzyme Q10 by sphingosine unit cell strain fermentation, extraction and coupling
  • Process for preparing coenzyme Q10 by sphingosine unit cell strain fermentation, extraction and coupling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Sphingomonas sp. (Sphingomonas sp.) ZUTE03 (CCTCC No. M207084) fermentation extraction coupled production of coenzyme Q 10 Process Validation

[0028] Production of Coenzyme Q by Fermentation-Extraction Coupled Method 10

[0029] Direct extraction of coenzyme Q produced by microbial fermentation by fermentation-extraction coupling method 10 There are two main problems to be solved: 1. Coenzyme Q 10 It is an intracellular product obtained by microbial fermentation, so it is necessary to choose a good cell permeabilizer to dissolve it from the cell, creating a prerequisite for the coupling extraction of fermentation extraction; 2. To select a suitable organic solvent to extract extracellular coenzyme Q 10 , to determine the optimal amount, optimal time and optimal extraction time of its addition, and the addition of this solvent has a significant effect on the growth of bacteria and coenzyme Q 10 output is not affected.

[0030] Therefore, the method is va...

Embodiment 2

[0032] Example 2: Sphingomonas sp. (Sphingomonas sp.) ZUTE03 CCTCC No.M207084 Fermentation Extraction Coupled Production of Coenzyme Q 10 Optimization of process conditions

[0033] On the basis of confirming the results of Example 1 and combining the mechanism of fermentation-extraction coupling, various conditions were explored and optimized.

[0034] (1), selection of fermentation extraction coupling extraction solvent

[0035] Select n-hexane, acetone, propylene glycol, and olive oil as the fermentation extraction reagent, and use soybean oil as the cell permeabilizing agent in advance (addition concentration 1%, 1.5mL), add fermentation medium (glucose 15g, (NH 4 ) 2 SO 4 10g, KH 2 PO 4 0.5g, Na 2 HPO 4 1.5g, MgSO 4 0.5g, 1000ml of water (pH 7.0) 150mL, inoculate the sphingomonas (Sphingomonas sp.) ZUTE03 (CCTCC No.M 207084) bacterial suspension (cell concentration 1.94g / L) at 4% (v / v) Amount, inoculated in the fermentation medium, culture temperature 25°C, 20...

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Abstract

The invention provides a method for making coenzyme Q10 by means of sphingosine monad fermentation, extraction and coupling. The method comprises the following steps that: sphingosine monad strain is inoculated in liquid medium suitable for sphingosine strain; soybean oil with the volume occupying 1 to 5 percent of that of the liquid medium is added in the liquid medium so as to carry out cultivation at a temperature of between 25 and 28 DEG C for 24 to 36 hours; then, normal hexane with the volume occupying 20 to 50 percent of that of the liquid medium is added in the liquid medium so as to carry out cultivation at a temperature of between 25 and 28 DEG C for 6 to 12 hours; after the reaction is completed, the reaction liquid undergoes delamination and then the organic layer is taken out so as to obtain the coenzyme Q10 after separation and purification. The method has the advantages of high recovery rate of extraction solvent, economization on manpower and material resources, simple and time-saving method and high yield of coenzyme Q10, and is propitious to industrialized production.

Description

(1) Technical field [0001] The invention relates to a method for preparing coenzyme Q by coupling fermentation and extraction of sphingomonas 10 The method, especially a method using Sphingomonas ZUTE03 (CCTCC NO: M 207084) coupled with fermentation and extraction to prepare coenzyme Q 10 Methods. (2) Background technology [0002] coenzyme Q 10 (CoenzymeQ 10 , CoQ 10 ) is widely present in animals, plants and microorganisms, and is an important hydrogen transporter in the respiratory chain of biological cells, as well as a good biochemical drug (NazzalS, Nutan M, Palamakulaa, et al.Optiimization of a self-nanoemulsified tablet dosage form of Ubiquinone using response surface methodology. International Journal of Pharmaceutics, 2002, 240: 103-114.). It has functions such as anti-oxidation, elimination of free radicals, and improvement of the body's immunity (Ernster L. Facts and ideas about the function of coenzyme Q 10 in the Mitochondria. Elservier Amsterdam. 1977, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12R1/01
Inventor 钟卫鸿方建军柳华贵钟莉李炎生李艺潇汪新张艾晓
Owner ZHEJIANG UNIV OF TECH
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