Phellinus polysaccharide II having antineoplastic activity, extraction and separation method thereof

A technology of polysaccharide and anti-tumor activity of P. acicularis, which is applied in the directions of organic active ingredients, anti-tumor drugs, and medical preparations containing active ingredients, etc., can solve problems such as large blindness, loss of active ingredients, and toxicity to normal cells , to achieve good anti-tumor activity and reduce blindness

Inactive Publication Date: 2008-12-17
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many defects in the chemical drugs currently used for cancer treatment: 1. Drugs often have toxicity to normal cells while acting on target cells, causing serious side effects such as edema, weight loss, fatigue, decreased white blood cell count, and poor liver function in patients 2. Most drugs have varying degrees of mutagenic genotoxicity, which increases the possibility of patients suffering from second tumors while treating tumors
[0004] At present, the extraction of Phellinus polysaccharides is mainly to separate and purify various monomer chemical components first, and then conduct activity tests. This extraction method makes the extraction and separation work very blind, and it is easy to cause active components during the separation process. lost

Method used

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  • Phellinus polysaccharide II having antineoplastic activity, extraction and separation method thereof
  • Phellinus polysaccharide II having antineoplastic activity, extraction and separation method thereof
  • Phellinus polysaccharide II having antineoplastic activity, extraction and separation method thereof

Examples

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Embodiment 1

[0031] Example 1: Get the fruiting body of the fungus, pulverize it, pass through a sieve with a diameter of 260 μm, get 1 kg of dry fruiting powder, add 10 kg, 5 kg and 2 kg of distilled water respectively, extract 3 times at 100° C. for 2 hours each time, combine the extracts, and use Rotary evaporator concentrated to 3kg. Slowly add 12kg of 90% ethanol to the concentrated solution, put the solution in a 4°C refrigerator, let it stand overnight (about 10 hours), then centrifuge at a speed of 4000r / min for 10min, take the precipitate, and dry it at a low temperature of 40°C. get crude polysaccharides.

[0032] Dissolve the crude polysaccharide in 0.01mol / L acetic acid-sodium acetate buffer solution with a pH value of 4.5, prepare a solution with a polysaccharide content of 10mg / ml, centrifuge at 4000r / min for 10min, discard the precipitate, and filter the centrifugate through a 0.45μm microporous membrane To remove impurities, the filtrate was loaded on a 2.6×20cm DEAE-Sepharo...

Embodiment 2

[0036] Example 2: Take the fruiting body of Phytophthora aciculatus, crush it, add 5 times the amount of water to the fruiting body powder, boil and extract 4 times, each time for 1 hour, combine the extracts, add 3 times the amount of 90% ethanol after concentration, and place at 4 °C In the refrigerator, let it stand overnight (about 7 hours), centrifuge at a speed of 3500r / min, and dry the precipitate at a low temperature of 40°C to obtain the crude polysaccharide.

[0037] Dissolve the crude polysaccharide in 0.01mol / L acetic acid-sodium acetate buffer solution with a pH value of 5.0, centrifuge at 3500r / min for 10min to discard the precipitate, filter the centrifugate through a 0.45μm microporous membrane to remove impurities, and load the filtrate on a 2.6×20cm DEAE-Sepharose Fast Flow column, 0.01mol / L acetic acid-sodium acetate buffer solution with a pH value of 5.0 elutes 2 column volumes, and the eluate is discarded or collected for use in antitumor experiments (the e...

Embodiment 3

[0039] Example 3: Take the fruiting body of Phylophora acutiformis, pulverize, add 10 times, 2 times, 5 times, and 3 times the amount of water to the fruiting body powder and boil for extraction 4 times, each time for 1.5 hours, combine the extracts, and concentrate Add 5 times the amount of 90% ethanol, put it in a refrigerator at 4°C, let it stand overnight (about 14 hours), centrifuge at a speed of 4500r / min, and dry the precipitate at a low temperature of 45°C to obtain crude polysaccharide.

[0040] Take the crude polysaccharide, dissolve it in the acetic acid-sodium acetate buffer solution with a pH value of 4.0, centrifuge at a speed of 4500r / min, discard the precipitate, filter the centrifugate through a 0.45 μm microporous membrane, put the filtrate on an ion exchange column, and use a pH value of 4.0 acetic acid-sodium acetate buffer was used to elute 2 column volumes, and the eluate was discarded or collected for anti-tumor experiments (the eluate was collected by a ...

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Abstract

The invention provides a Phellinus polysaccharide and an extraction and separation method. Firstly, crude polysaccharide is extracted by a poach and ethyl alcohol deposition method and then separated and purified with an ion-exchange method. Acetic acid-sodium acetate buffer solution with pH value of 4 to 5 is used for elution and then discarded and then acetic acid-sodium acetate buffer solution with pH value of 4 to 5 and containing the NaCl with the concentration of more than or equal to 0.2mol / L is used for elution. Eluent is dialyzed and desalted in a dialysis bag and then concentrated and dried at low temperature to obtain Phellinus polysaccharide II with high anti-tumor activity. Compared with the prior art, the invention adopts an activity tracing and separating method to separate and sublimate water-solubility active polysaccharides with the anti-tumor function from the phellinus sporocarp, which can greatly reduce the aimlessness in separation and losses of active ingredients caused in the process of separation in comparison with the conventional method that monomer chemical composition is firstly separated and sublimated and a vitality test method is then carried out.

Description

technical field [0001] The invention relates to the technical field of Acupunculatus polysaccharides, in particular to Acupunculatus polysaccharide II with anti-tumor activity and its extraction and separation method. Background technique [0002] Since entering the 21st century, cancer has become the number one killer of human health. According to statistics, nearly 2 million new cancer patients are added every year in my country, and about 1.5 million people die from cancer every year, while the incidence of cancer is still increasing at a rate of 2.5% per year. According to a research report released by the International Cancer Research Center (IARC) in October 2005: According to the current trend of cancer incidence, the incidence of cancer worldwide will increase by 50% by 2020, and the number of new cancer patients in the world will increase every year. Reaching 15 million people, it can be seen that it is urgent to develop anti-tumor drugs with high efficiency and lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 陶美华章卫民郑爱珠陈玉婵潘清灵李浩华王磊
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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