Disease-resistant microorganism additive for livestock and poultry, and preparation thereof

A microbial additive, livestock and poultry technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reducing the mortality rate, increasing the production and quality of livestock and poultry, to promote digestion and absorption, and enhance immunity The effect of enhancing the immunity of poultry

Inactive Publication Date: 2008-12-17
WUHAN NONGDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the drug treatment after the onset of livestock and poultry is passive, and it is difficult to achieve the purpose of reducing the mortality rate and increasing the output and quality of livestock and poultry.

Method used

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  • Disease-resistant microorganism additive for livestock and poultry, and preparation thereof
  • Disease-resistant microorganism additive for livestock and poultry, and preparation thereof
  • Disease-resistant microorganism additive for livestock and poultry, and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of this microbial additive (aqua), it comprises the steps:

[0025] ①. Strain cultivation: transplant Bacillus licheniformis SHK3 and Bacillus licheniformis SHK3 strains to nutrient agar slant medium, place them in a 36°C incubator, and cultivate them for 24 hours to rejuvenate Bacillus licheniformis;

[0026] ②. Shaker culture: Take the above slant culture medium and inoculate it into 5 bottles of 500 ml Erlenmeyer flasks, each bottle of Erlenmeyer flask is filled with 120 ml of nutrient broth culture medium, each bottle is inoculated with 3 rings, and after inoculation, move to the shaker for 18 Hours, the temperature is 36 ℃, then inoculated to 50 liters of seed fermentation culture, and 35 liters of culture fluid are installed in the fermenter;

[0027] ③. Cultivation in 50-liter seed fermenter: use rapeseed oil or soybean oil as defoamer, sterilize at 121°C for 30 minutes, and when it is cooled to 37°C, the air ratio is 0.4:1, the speed is 30...

Embodiment 2

[0031] The preparation method of this microbial additive (aqua), it comprises the steps:

[0032] ①. Strain cultivation: transplant Bacillus licheniformis SHK3 and Bacillus licheniformis SHK3 strains to nutrient agar slant medium, place them in a 36°C incubator, and cultivate them for 24 hours to rejuvenate Bacillus licheniformis;

[0033] ② Shaker culture: Take the above slant culture medium and inoculate it into 5 bottles of 500 ml Erlenmeyer flasks, each bottle of Erlenmeyer flask is equipped with 120 ml of nutrient broth culture medium, each bottle is inoculated with 3 loops, and after inoculation, move to the shaker for 24 Hours, the temperature is 36 ℃, then inoculated to 50 liters of seed fermentation culture, and 35 liters of culture fluid are installed in the fermenter;

[0034] ③. Cultivation in 50-liter seed fermenter: use rapeseed oil or soybean oil as defoamer, sterilize at 121°C for 30 minutes, and when it is cooled to 37°C, the air ratio is 2:1, the speed is 500...

Embodiment 3

[0038] The preparation method of this microbial additive (aqua), it comprises the steps:

[0039] ①. Strain cultivation: transplant Bacillus licheniformis SHK3 and Bacillus licheniformis SHK3 strains to nutrient agar slant medium, place them in a 36°C incubator, and cultivate them for 24 hours to rejuvenate Bacillus licheniformis;

[0040] ② Shaker culture: Inoculate 5 bottles of 500 ml Erlenmeyer flasks with the above slant medium strains, each bottle contains 120 milliliters of nutrient broth culture medium, inoculate 3 rings in each bottle, move to shaker culture after inoculation 21 Hours, the temperature is 36 ℃, then inoculated to 50 liters of seed fermentation culture, and 35 liters of culture fluid are installed in the fermenter;

[0041] ③. Cultivation in 50-liter seed fermenter: use rapeseed oil or soybean oil as defoamer, sterilize at 121°C for 30 minutes, and when it is cooled to 37°C, the air ratio is 1.2:1, the speed is 400r / min, and the temperature is 36°C Unde...

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Abstract

The invention relates to a livestock and poultry antiviral microorganism additive which is obtained by the fermentation of Bacillus licheniformis SHK3, with the strain accession number of CCTCC NO: 206115. The additive overcomes the disadvantages that in the current livestock and poultry breeding course, livestock and poultry still usually suffer from virosis, bacteriosis, and intestinal diseases, etc. caused by infection of digestive canal or the infection of foreign objects, and no good prevention and cure measures exist. The invention is a purely natural antiviral microorganism additive which integrates antibiosis and antivirus, enhances the immunity of the livestock and poultry and improves the output and the quality of the livestock and poultry. The invention also discloses a method for preparing the livestock and poultry antiviral microorganism additive (aqua and pulvis).

Description

technical field [0001] The invention relates to a livestock and poultry disease-resistant microbial additive. The invention also relates to a preparation method of the microbial additive. Background technique [0002] Livestock and poultry breeding has a history of more than 5,000 years in my country. It is the earliest traditional breeding industry in China. It has continued to this day along with the progress of human history. Under the current market economy, my country's livestock and poultry breeding has made great progress, and governments around the world have given Highly valued, scientific and technological circles have also carried out continuous research and exploration on scientific breeding, and have made great progress in seed selection, disease prevention and other aspects. However, in the breeding process, viral, bacterial diseases and enteric diseases caused by livestock and poultry infections through the digestive tract or foreign objects are still frequent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/10A23K10/18A23K40/10
CPCY02A40/70
Inventor 黄江红
Owner WUHAN NONGDA BIOTECH
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